FastQCFastQC Report
Thu 2 Feb 2017
SRR4062398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836350
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG50660.6057272672924015No Hit
TCCATGTACTCTGCGTTGATACCAC42800.5117474741436001No Hit
GAGTACATGGAAGCAGTGGTATCAA24750.29592873796855385No Hit
CATGTACTCTGCGTTGATACCACTG20110.24044957254737848No Hit
GTATCAACGCAGAGTACTTTTTTTT18370.21964488551443773No Hit
GCTTCCATGTACTCTGCGTTGATAC15610.1866443474621869No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15090.18042685478567586No Hit
CCCATGTACTCTGCGTTGATACCAC12440.1487415555688408No Hit
GCGTTGATACCACTGCTTCCATGTA12390.1481437197345609No Hit
TATCAACGCAGAGTACTTTTTTTTT12190.14575237639744126No Hit
CATGGAAGCAGTGGTATCAACGCAG12010.14360016739403358No Hit
GTACATGGGAAGCAGTGGTATCAAC11370.1359478687152508No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10140.12124110719196508No Hit
GTATCAACGCAGAGTACATGGAAGC9340.11167573384348657No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9000.10761045017038322No Hit
ACGCAGAGTACATGGAAGCAGTGGT8650.10342559933042388No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8470.10127339032701621No Hit
GCGCAAGACGGACCAGAGCGAAAGC8410.10055598732588032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG953.0377123E-1015.000236513
ACGCCCC456.7507644E-414.77977917
CCTATGC653.3754422E-614.6121173
CAACCCG1058.913048E-1114.47641810
CGTGCGT400.005273754214.25107814
ACCCGGT1006.566552E-1014.25022512
CCGATAA602.565077E-514.2502249
CGACCAT1203.6379788E-1214.25022410
CGTTTCC551.9571897E-413.81922614
ATAACGA551.9581837E-413.81839912
GATAACG551.959178E-413.81757311
CCGACCA1450.013.7588389
CGGTGAG1051.364242E-913.572453515
CCGGTGA1051.364242E-913.572453514
GCAAGAC1351.8189894E-1213.3673813
CGGACCG500.001498357413.300215
GTCTTAA655.4641783E-513.1493311
GCATCGC1102.73576E-912.95552414
GACCATA1251.0186341E-1012.9194311
CAAGACG1550.012.8719414
Produced by FastQC (version 0.11.5)