##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062398_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	836350
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13830573324565	32.0	32.0	32.0	32.0	32.0
2	30.822594607520774	32.0	32.0	32.0	32.0	32.0
3	30.81365696179829	32.0	32.0	32.0	32.0	32.0
4	30.87799007592515	32.0	32.0	32.0	32.0	32.0
5	30.686032163567884	32.0	32.0	32.0	32.0	32.0
6	34.31569558199318	36.0	36.0	36.0	32.0	36.0
7	34.17579960542835	36.0	36.0	36.0	32.0	36.0
8	34.18480779577928	36.0	36.0	36.0	32.0	36.0
9	34.330008967537516	36.0	36.0	36.0	32.0	36.0
10	34.029635917976925	36.0	36.0	36.0	32.0	36.0
11	34.33348358940635	36.0	36.0	36.0	32.0	36.0
12	34.12190111795301	36.0	36.0	36.0	32.0	36.0
13	34.22943743647994	36.0	36.0	36.0	32.0	36.0
14	34.14189633526634	36.0	36.0	36.0	32.0	36.0
15	34.083932564117895	36.0	36.0	36.0	32.0	36.0
16	34.0836396245591	36.0	36.0	36.0	32.0	36.0
17	34.009475697973336	36.0	36.0	36.0	32.0	36.0
18	34.02296287439469	36.0	36.0	36.0	32.0	36.0
19	34.00929873856639	36.0	36.0	36.0	32.0	36.0
20	33.99518024750404	36.0	36.0	36.0	32.0	36.0
21	33.95001135888085	36.0	36.0	36.0	32.0	36.0
22	33.895917976923535	36.0	36.0	36.0	32.0	36.0
23	33.83576373527829	36.0	36.0	36.0	32.0	36.0
24	33.81948107849585	36.0	36.0	36.0	32.0	36.0
25	33.322111556166675	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	30.0
5	78.0
6	149.0
7	45.0
8	130.0
9	165.0
10	91.0
11	35.0
12	50.0
13	46.0
14	163.0
15	205.0
16	301.0
17	457.0
18	617.0
19	865.0
20	1227.0
21	1778.0
22	2527.0
23	3612.0
24	5260.0
25	7131.0
26	9795.0
27	13020.0
28	17288.0
29	23436.0
30	30169.0
31	41803.0
32	60188.0
33	86277.0
34	188147.0
35	341256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.01500211651094	16.7211538691494	10.80723966317582	25.456604351163843
2	16.26149830387687	20.12533494994135	37.27381537427704	26.33935137190474
3	19.83104351257309	24.202389066255336	27.87650512369815	28.090062297473423
4	12.394435927301602	15.117636225786931	35.73177936002641	36.75614848688506
5	14.10609262920687	36.865687530426065	33.94789502453821	15.080324815828853
6	34.599455940512335	34.707956810194055	17.053657089401366	13.63893015989224
7	29.801930679327278	30.568291679030544	20.97005389055336	18.65972375108882
8	28.23290899277261	33.03489470074955	19.925416272225817	18.80678003425203
9	27.04262808352553	14.431336315077035	18.429402211054313	40.09663339034312
10	15.255284229730071	27.213438948098013	32.46121745875745	25.070059363414465
11	37.38858017080184	21.21854926175011	21.71654172961403	19.67632883783402
12	23.87665672928415	23.87426217927996	29.561198711732096	22.687882379703794
13	30.03906105039869	19.550516604238418	25.330067671504107	25.080354673858785
14	23.444802520686483	19.628936178363467	25.299163921238126	31.62709737971192
15	25.338490138268927	27.104348690030296	23.170668936235415	24.38649223546536
16	25.712127686825415	25.95009210946842	24.58790140037514	23.749878803331022
17	23.67791296103203	25.99185708111913	26.243734489607064	24.08649546824178
18	24.93843271297133	24.6690571255829	27.3233045799836	23.06920558146217
19	24.825531069178172	26.40374307541823	25.321311369423967	23.44941448597963
20	24.656756941177317	24.487125616963613	26.713521783319628	24.14259565853945
21	25.683462453413213	25.13178389237479	24.37597947487576	24.808774179336236
22	24.3890786127223	25.512613574860023	25.38556779048103	24.712740021936654
23	23.556062686856666	25.076418913336823	25.439206704031015	25.9283116957755
24	23.640453904377416	26.436649497989805	25.425804736830766	24.49709186080202
25	24.76743824093518	24.999940138543355	25.08266867162639	25.149952948895077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	126.0
2	315.0
3	504.0
4	504.0
5	504.0
6	1442.5
7	2381.0
8	2381.0
9	2381.0
10	2398.0
11	2415.0
12	2415.0
13	2415.0
14	2527.5
15	2640.0
16	2640.0
17	2640.0
18	4168.0
19	5696.0
20	5696.0
21	5696.0
22	9184.5
23	12673.0
24	12673.0
25	12673.0
26	18977.5
27	25282.0
28	25282.0
29	25282.0
30	32073.5
31	38865.0
32	38865.0
33	38865.0
34	46071.0
35	53277.0
36	53277.0
37	53277.0
38	59815.5
39	66354.0
40	66354.0
41	66354.0
42	77672.5
43	88991.0
44	88991.0
45	88991.0
46	107196.5
47	125402.0
48	125402.0
49	125402.0
50	118243.0
51	111084.0
52	111084.0
53	111084.0
54	100264.0
55	89444.0
56	89444.0
57	89444.0
58	81983.5
59	74523.0
60	74523.0
61	74523.0
62	65831.0
63	57139.0
64	57139.0
65	57139.0
66	47551.5
67	37964.0
68	37964.0
69	37964.0
70	28658.0
71	19352.0
72	19352.0
73	19352.0
74	15081.5
75	10811.0
76	10811.0
77	10811.0
78	8722.5
79	6634.0
80	6634.0
81	6634.0
82	4649.0
83	2664.0
84	2664.0
85	2664.0
86	1948.5
87	1233.0
88	1233.0
89	1233.0
90	836.0
91	439.0
92	439.0
93	439.0
94	260.5
95	82.0
96	82.0
97	82.0
98	228.5
99	375.0
100	375.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008130567346206732
2	0.0037065821725354216
3	0.004782686674239254
4	0.015304597357565614
5	0.036707120224786276
6	0.049261672744664316
7	0.06958809111018115
8	0.09314282298080948
9	0.0981646439887607
10	0.11801279368685359
11	0.11908889818855742
12	0.1339152268786991
13	0.1184910623542775
14	0.12219764452681292
15	0.1134692413463263
16	0.11131703234291863
17	0.12231721169366891
18	0.12973037603873977
19	0.13164345070843544
20	0.12064327135768518
21	0.12925210737131584
22	0.14563280923058528
23	0.13762180905123453
24	0.13248042087642733
25	0.1300890775393077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	836350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.89429770469449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09402955240343	44.767995321706344
2	12.440802746807922	13.907398648309258
3	3.4931902779495214	5.85748252004565
4	1.3868700911079712	3.100725190005028
5	0.6983470527433696	1.951680903361694
6	0.381593524428417	1.2797341233951325
7	0.27154892049276164	1.0624625342387581
8	0.16631260953814547	0.7436741207655772
9	0.13237042290724768	0.6658876642746611
>10	0.7533144441642604	8.021494632195946
>50	0.08555742844285459	3.3869404885390844
>100	0.08684260324293476	9.703098703333254
>500	0.006432785421774138	2.460082312342696
>1k	0.002573114168709655	2.484172565912845
>5k	2.1442618072580456E-4	0.6071702715740085
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5066	0.6057272672924015	No Hit
TCCATGTACTCTGCGTTGATACCAC	4280	0.5117474741436001	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2475	0.29592873796855385	No Hit
CATGTACTCTGCGTTGATACCACTG	2011	0.24044957254737848	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1837	0.21964488551443773	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1561	0.1866443474621869	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1509	0.18042685478567586	No Hit
CCCATGTACTCTGCGTTGATACCAC	1244	0.1487415555688408	No Hit
GCGTTGATACCACTGCTTCCATGTA	1239	0.1481437197345609	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1219	0.14575237639744126	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1201	0.14360016739403358	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1137	0.1359478687152508	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1014	0.12124110719196508	No Hit
GTATCAACGCAGAGTACATGGAAGC	934	0.11167573384348657	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	900	0.10761045017038322	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	865	0.10342559933042388	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	847	0.10127339032701621	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	841	0.10055598732588032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1956716685598135E-4
2	0.0	0.0	0.0	0.0	1.1956716685598135E-4
3	0.0	0.0	0.0	0.0	1.1956716685598135E-4
4	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
5	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
6	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
7	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
8	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
9	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
10	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
11	0.0	0.0	0.0	1.1956716685598135E-4	1.1956716685598135E-4
12	0.0	0.0	0.0	1.1956716685598135E-4	2.391343337119627E-4
13	0.0	0.0	0.0	1.1956716685598135E-4	2.391343337119627E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	95	3.0377123E-10	15.0002365	13
ACGCCCC	45	6.7507644E-4	14.779779	17
CCTATGC	65	3.3754422E-6	14.612117	3
CAACCCG	105	8.913048E-11	14.476418	10
CGTGCGT	40	0.0052737542	14.251078	14
ACCCGGT	100	6.566552E-10	14.250225	12
CCGATAA	60	2.565077E-5	14.250224	9
CGACCAT	120	3.6379788E-12	14.250224	10
CGTTTCC	55	1.9571897E-4	13.819226	14
ATAACGA	55	1.9581837E-4	13.818399	12
GATAACG	55	1.959178E-4	13.817573	11
CCGACCA	145	0.0	13.758838	9
CGGTGAG	105	1.364242E-9	13.5724535	15
CCGGTGA	105	1.364242E-9	13.5724535	14
GCAAGAC	135	1.8189894E-12	13.367381	3
CGGACCG	50	0.0014983574	13.30021	5
GTCTTAA	65	5.4641783E-5	13.149331	1
GCATCGC	110	2.73576E-9	12.955524	14
GACCATA	125	1.0186341E-10	12.91943	11
CAAGACG	155	0.0	12.871941	4
>>END_MODULE