FastQCFastQC Report
Thu 2 Feb 2017
SRR4062398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836350
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21860.26137382674717524No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18670.22323190052011718No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9610.11490404734859808No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9510.11370837568003826No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8490.10151252466072816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1950.023.6922114
ATCGGGG500.002579840121.9999124
CCGTCGT1650.021.3332469
GGACGTG2000.020.8999146
CGTGATT956.8613735E-720.8420228
CAACGTA654.930552E-420.3076120
ATCGTTT2300.020.08687429
GTGTATC550.004477930720.0035061
ATACCGT1653.6379788E-1219.999926
GTCGGAA2100.019.90468239
GATATAC2600.019.464951
TACCGTC1705.456968E-1219.4116867
CATCGTT2300.019.13035828
CGTCGTA1851.8189894E-1219.02694910
TCGTTTA2450.018.85706530
GATTTCG951.596207E-518.5262441
GTCTTAG1456.2846084E-918.2100871
CGCAATA2300.018.1738436
ACCGTCG1951.8189894E-1218.0512088
GTGTTAT1355.5084456E-817.9290681
Produced by FastQC (version 0.11.5)