FastQCFastQC Report
Thu 2 Feb 2017
SRR4062397_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062397_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178580
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG88380.4056770924179971No Hit
TCCATGTACTCTGCGTTGATACCAC78010.3580772796959487No Hit
GTATCAACGCAGAGTACTTTTTTTT61780.28357921214736204No Hit
TATCAACGCAGAGTACTTTTTTTTT44830.20577623956889352No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44800.2056385351926484No Hit
GAGTACATGGAAGCAGTGGTATCAA41300.18957302463072276No Hit
CATGTACTCTGCGTTGATACCACTG37670.17291079510506843No Hit
GAGTACTTTTTTTTTTTTTTTTTTT33590.15418299993573797No Hit
GCGCAAGACGGACCAGAGCGAAAGC30440.13972404043000486No Hit
CCCATGTACTCTGCGTTGATACCAC28470.13068145305657813No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27830.12774375969668317No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27800.1276060553204381No Hit
GTACATGGGAAGCAGTGGTATCAAC26370.12104214671942275No Hit
GAATAACGCCGCCGCATCGCCAGTC25930.11902248253449495No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25910.11893067961699824No Hit
GAACTACGACGGTATCTGATCGTCT25100.11521266145838115No Hit
GAATAGGACCGCGGTTCTATTTTGT24830.11397332207217545No Hit
GCTTCCATGTACTCTGCGTTGATAC24700.1133766031084468No Hit
GTATCTGATCGTCTTCGAACCTCCG24400.11199955934599601No Hit
GATTAAGAGGGACGGCCGGGGGCAT22430.10295697197256928No Hit
GTCCTATTCCATTATTCCTAGCTGC21960.10079960341139642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG6000.013.1400785
GCGTTAT2700.013.01036551
CGTTATT2800.012.8862632
CGGTCCA5450.012.72557210
CAAGACG6350.012.4143934
CGAACGA1850.012.32708416
ACGGTAT5250.012.3052549
CGCATCG4600.012.1861113
GCGCGCT1800.012.1404915
ATCGCCA4700.012.13037516
AGGCCCG5350.011.89794410
ATATACG4600.011.7719386
TACGCTA4550.011.6927759
GCGCAAG6600.011.6517891
ACTCTAA4750.011.6007310
ACGCTAT4700.011.52200110
AACCGCG2150.011.48883157
CGCAAGA6200.011.4860562
ATACGCT4850.011.3612798
GTTATTC3350.011.3385233
Produced by FastQC (version 0.11.5)