##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062397_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2178580
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179723030597913	32.0	32.0	32.0	32.0	32.0
2	30.882187479918112	32.0	32.0	32.0	32.0	32.0
3	30.86802917496718	32.0	32.0	32.0	32.0	32.0
4	30.925299048003744	32.0	32.0	32.0	32.0	32.0
5	30.754021885815533	32.0	32.0	32.0	32.0	32.0
6	34.39235603007464	36.0	36.0	36.0	32.0	36.0
7	34.26693488419062	36.0	36.0	36.0	32.0	36.0
8	34.26977756153091	36.0	36.0	36.0	32.0	36.0
9	34.40961773265154	36.0	36.0	36.0	32.0	36.0
10	34.11155615125449	36.0	36.0	36.0	32.0	36.0
11	34.412735818744316	36.0	36.0	36.0	32.0	36.0
12	34.19611398250236	36.0	36.0	36.0	32.0	36.0
13	34.32261151759403	36.0	36.0	36.0	32.0	36.0
14	34.22668756713088	36.0	36.0	36.0	32.0	36.0
15	34.17972027651039	36.0	36.0	36.0	32.0	36.0
16	34.17913778699887	36.0	36.0	36.0	32.0	36.0
17	34.09448218564386	36.0	36.0	36.0	32.0	36.0
18	34.11015018957303	36.0	36.0	36.0	32.0	36.0
19	34.11350466817836	36.0	36.0	36.0	32.0	36.0
20	34.09789817220391	36.0	36.0	36.0	32.0	36.0
21	34.06343030781518	36.0	36.0	36.0	32.0	36.0
22	34.022958073607576	36.0	36.0	36.0	32.0	36.0
23	33.983276262519624	36.0	36.0	36.0	32.0	36.0
24	33.960717990617745	36.0	36.0	36.0	32.0	36.0
25	33.51220657492495	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	79.0
5	202.0
6	394.0
7	98.0
8	299.0
9	397.0
10	251.0
11	73.0
12	157.0
13	132.0
14	360.0
15	417.0
16	732.0
17	917.0
18	1239.0
19	1772.0
20	2738.0
21	3997.0
22	5992.0
23	8778.0
24	12284.0
25	17083.0
26	24021.0
27	31589.0
28	43172.0
29	57690.0
30	75294.0
31	104226.0
32	151018.0
33	219671.0
34	493611.0
35	919884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63732171145982	17.51034350109552	11.209908461220305	25.64242632622436
2	16.90958432253883	20.20283532459711	36.23103984547279	26.65654050739127
3	19.12257967378127	23.873375757467258	28.468145406171374	28.535899162580098
4	12.836213375128025	15.042554471054284	35.3542517974151	36.76698035640259
5	14.743614762992143	36.17637456567348	33.33400679677674	15.746003874557637
6	34.5076408670778	34.7134209877354	16.774409237465484	14.004528907721323
7	30.09795563656006	30.577444940719523	20.87393000255385	18.45066942016657
8	28.523997140594194	31.8776427911182	19.948398383590394	19.649961684697217
9	27.16282570171372	14.434689199551757	18.998177343092763	39.40430775564176
10	16.079480594918817	26.341251123606867	31.361728801283807	26.21753948019051
11	37.13497766770818	21.39037875511777	21.90205225466763	19.57259132250642
12	24.335715375663458	24.031081148160336	28.48633374453745	23.146869731638755
13	29.139144369642317	19.316045355550624	25.665555894102976	25.87925438070408
14	23.67380591174163	19.810589331253585	24.97128783537164	31.544316921633143
15	25.39277276965403	26.73143298817948	23.00656532166492	24.86922892050157
16	25.91450931844845	25.795584621952266	23.926104935880378	24.363801123718908
17	24.01391226339915	25.821743610294856	25.67858590519329	24.485758221112704
18	25.113431628488435	24.299325293230872	26.840873993455162	23.746369084825535
19	24.98127068842055	25.7940079072166	25.766476968232332	23.45824443613052
20	25.039259273897603	24.10250565393289	26.043846301263972	24.814388770905534
21	26.364396384007748	24.57253241252903	24.555067403444927	24.50800380001829
22	25.12507142505805	24.706151679931157	25.224687258229338	24.94408963678146
23	23.731910371417698	24.36696642145732	25.81836551208059	26.082757695044396
24	24.018175558874074	25.819598477525762	25.41916991968907	24.74305604391109
25	24.694246174917385	24.598463992057983	25.23947843751867	25.467811395505958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	299.0
1	299.0
2	876.0
3	1453.0
4	1453.0
5	1453.0
6	4205.0
7	6957.0
8	6957.0
9	6957.0
10	7112.0
11	7267.0
12	7267.0
13	7267.0
14	7169.5
15	7072.0
16	7072.0
17	7072.0
18	11362.0
19	15652.0
20	15652.0
21	15652.0
22	24780.5
23	33909.0
24	33909.0
25	33909.0
26	49521.5
27	65134.0
28	65134.0
29	65134.0
30	78195.5
31	91257.0
32	91257.0
33	91257.0
34	112309.5
35	133362.0
36	133362.0
37	133362.0
38	150087.0
39	166812.0
40	166812.0
41	166812.0
42	193705.0
43	220598.0
44	220598.0
45	220598.0
46	266910.5
47	313223.0
48	313223.0
49	313223.0
50	303018.5
51	292814.0
52	292814.0
53	292814.0
54	265610.0
55	238406.0
56	238406.0
57	238406.0
58	219774.5
59	201143.0
60	201143.0
61	201143.0
62	178908.0
63	156673.0
64	156673.0
65	156673.0
66	130288.5
67	103904.0
68	103904.0
69	103904.0
70	79446.5
71	54989.0
72	54989.0
73	54989.0
74	43391.0
75	31793.0
76	31793.0
77	31793.0
78	26351.0
79	20909.0
80	20909.0
81	20909.0
82	14668.0
83	8427.0
84	8427.0
85	8427.0
86	6210.0
87	3993.0
88	3993.0
89	3993.0
90	2690.5
91	1388.0
92	1388.0
93	1388.0
94	812.0
95	236.0
96	236.0
97	236.0
98	573.0
99	910.0
100	910.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007298331940989084
2	0.0029376933598949774
3	0.004727850251080979
4	0.014367156588236375
5	0.035940842199965115
6	0.046498177712087696
7	0.06756694727758447
8	0.08739637745687558
9	0.09579634440782528
10	0.11714052272581221
11	0.11856346794701135
12	0.13109456618531337
13	0.12012411754445557
14	0.12269459923436367
15	0.11241267247473126
16	0.11076021995979031
17	0.12200607735313829
18	0.12944211367037245
19	0.13063555159782977
20	0.12214378172938337
21	0.12856998595415361
22	0.14794040154596114
23	0.13880601125503766
24	0.13361914641647313
25	0.12898309908288885
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2178580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.844618075468134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94403704328636	37.77047301671644
2	12.66594060669767	12.119941817880262
3	3.660778384051596	5.25445630931634
4	1.5206795777017128	2.910253344412104
5	0.804532449800447	1.9246273895011563
6	0.4890085985309671	1.4037857779400427
7	0.31623667286727697	1.0591155984354135
8	0.23488872772630146	0.8990529174638011
9	0.17630157444405462	0.7591573345841556
>10	0.9701844875067376	8.466636341831794
>50	0.08877138087063681	2.9593066982850065
>100	0.10330003577974181	10.89790302060703
>500	0.016765171742058466	5.607256331719743
>1k	0.008286234309293265	6.916192380413379
>5k	2.890546852079046E-4	1.051841720893241
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8838	0.4056770924179971	No Hit
TCCATGTACTCTGCGTTGATACCAC	7801	0.3580772796959487	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6178	0.28357921214736204	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4483	0.20577623956889352	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4480	0.2056385351926484	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4130	0.18957302463072276	No Hit
CATGTACTCTGCGTTGATACCACTG	3767	0.17291079510506843	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3359	0.15418299993573797	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3044	0.13972404043000486	No Hit
CCCATGTACTCTGCGTTGATACCAC	2847	0.13068145305657813	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2783	0.12774375969668317	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2780	0.1276060553204381	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2637	0.12104214671942275	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2593	0.11902248253449495	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2591	0.11893067961699824	No Hit
GAACTACGACGGTATCTGATCGTCT	2510	0.11521266145838115	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2483	0.11397332207217545	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2470	0.1133766031084468	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2440	0.11199955934599601	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2243	0.10295697197256928	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2196	0.10079960341139642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.180291749671804E-5
2	0.0	0.0	0.0	0.0	9.180291749671804E-5
3	0.0	0.0	0.0	0.0	9.180291749671804E-5
4	0.0	0.0	0.0	0.0	9.180291749671804E-5
5	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
6	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
7	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
8	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
9	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
10	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
11	0.0	0.0	0.0	4.590145874835902E-5	1.3770437624507708E-4
12	0.0	0.0	0.0	4.590145874835902E-5	2.2950729374179513E-4
13	0.0	0.0	0.0	4.590145874835902E-5	2.7540875249015416E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	600	0.0	13.140078	5
GCGTTAT	270	0.0	13.0103655	1
CGTTATT	280	0.0	12.886263	2
CGGTCCA	545	0.0	12.725572	10
CAAGACG	635	0.0	12.414393	4
CGAACGA	185	0.0	12.327084	16
ACGGTAT	525	0.0	12.305254	9
CGCATCG	460	0.0	12.18611	13
GCGCGCT	180	0.0	12.14049	15
ATCGCCA	470	0.0	12.130375	16
AGGCCCG	535	0.0	11.897944	10
ATATACG	460	0.0	11.771938	6
TACGCTA	455	0.0	11.692775	9
GCGCAAG	660	0.0	11.651789	1
ACTCTAA	475	0.0	11.60073	10
ACGCTAT	470	0.0	11.522001	10
AACCGCG	215	0.0	11.4888315	7
CGCAAGA	620	0.0	11.486056	2
ATACGCT	485	0.0	11.361279	8
GTTATTC	335	0.0	11.338523	3
>>END_MODULE