FastQCFastQC Report
Thu 2 Feb 2017
SRR4062397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178580
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA46080.2115139219124384No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40120.1841566524984164No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29350.13472078142643373No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26650.1223273875643768No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26320.12081263942568093No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA24110.1106684170422936No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24090.11057661412479688No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23420.10750121638865684No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC22750.1044258186525168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA956.871833E-720.84200744
ATACCGT8350.018.969976
TACCGTC8600.018.4185187
CGTCGTA8400.018.33324610
ACCGTCG8750.017.8513458
TAATACG750.001291361817.5999184
CCGTCGT9050.017.2595889
TAGGACG2600.016.9229974
GTAAGAC1602.1318556E-816.4999243
GTCGTAG9300.016.32250411
TAGACCG953.3479763E-416.210455
CGAGCCG9000.015.6443715
CAGATAC10350.015.5168363
GATTAAT9200.015.304275538
TCAGATA10500.015.2958692
CGCAATA8800.015.24992936
ATAATAC1756.416849E-815.0856433
AGATACC10400.015.019164
TTGATTA9400.014.97865236
CGACCAT10000.014.95992921
Produced by FastQC (version 0.11.5)