FastQCFastQC Report
Thu 2 Feb 2017
SRR4062396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551788
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26340.16973968093579794No Hit
GCGCAAGACGGACCAGAGCGAAAGC22730.14647619391308608No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20920.1348122295055768No Hit
GAATAACGCCGCCGCATCGCCAGTC19770.12740142339030847No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19750.1272725398056951No Hit
GATTAAGAGGGACGGCCGGGGGCAT19140.12334159047498755No Hit
GAACTACGACGGTATCTGATCGTCT19090.12301938151345415No Hit
GTATCTGATCGTCTTCGAACCTCCG18810.1212150113288671No Hit
GAATAGGACCGCGGTTCTATTTTGT18750.120828360575027No Hit
GGGTAGGCACACGCTGAGCCAGTCA17320.11161318427517161No Hit
ATCAGATACCGTCGTAGTTCCGACC17250.11116209172902483No Hit
GTCCTATTCCATTATTCCTAGCTGC16830.10845553645214424No Hit
TCGTAGTTCCGACCATAAACGATGC16530.10652228268294381No Hit
TATCAACGCAGAGTACTTTTTTTTT16280.10491123787527677No Hit
CTATTGGAGCTGGAATTACCGCGGC15900.1024624497676229No Hit
CTCTTAATCATGGCCTCAGTTCCGA15750.10149582288302267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG804.311005E-1016.6264413
CGTTATT1750.014.1073132
ACGCTTA1001.0031727E-813.30372817
TAGGTTG500.001500388313.2990075
CGGTCCA3750.013.17362510
TTAATAC604.1068712E-412.6612253
GCGTTAT1950.012.6575531
CGCATCG2750.012.43744113
GTATCAA20550.012.4265741
CGACGCT1001.4401667E-712.35146815
TCGACGC1001.4401667E-712.35146814
AATCGTA1001.440767E-712.35106913
AAGACGG4200.012.2133735
CAAGACG4200.012.2121924
GCATCGC2850.012.00142714
GCAAGAC4600.011.9731163
GCTCGTA1052.717734E-711.7625459
ATAACGA1950.011.69256612
CGACCAT3200.011.57838110
ATCGCCA3050.011.52782116
Produced by FastQC (version 0.11.5)