##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062396_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551788
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308361064784624	32.0	32.0	32.0	32.0	32.0
2	31.06483295398598	32.0	32.0	32.0	32.0	32.0
3	31.04583486919605	32.0	32.0	32.0	32.0	32.0
4	31.09193717183017	32.0	32.0	32.0	32.0	32.0
5	30.96855498302603	32.0	32.0	32.0	32.0	32.0
6	34.57985884669813	36.0	36.0	36.0	32.0	36.0
7	34.508468295927024	36.0	36.0	36.0	32.0	36.0
8	34.52905680415108	36.0	36.0	36.0	32.0	36.0
9	34.63428380680866	36.0	36.0	36.0	32.0	36.0
10	34.40560566262917	36.0	36.0	36.0	32.0	36.0
11	34.59484156340943	36.0	36.0	36.0	32.0	36.0
12	34.440830190721925	36.0	36.0	36.0	32.0	36.0
13	34.544197403253534	36.0	36.0	36.0	32.0	36.0
14	34.46496235310494	36.0	36.0	36.0	32.0	36.0
15	34.41376270469935	36.0	36.0	36.0	32.0	36.0
16	34.40403392731481	36.0	36.0	36.0	32.0	36.0
17	34.34524174693966	36.0	36.0	36.0	32.0	36.0
18	34.35078309666011	36.0	36.0	36.0	32.0	36.0
19	34.34702936225825	36.0	36.0	36.0	32.0	36.0
20	34.337228410066324	36.0	36.0	36.0	32.0	36.0
21	34.31704846280549	36.0	36.0	36.0	32.0	36.0
22	34.27314362528902	36.0	36.0	36.0	32.0	36.0
23	34.251738639556436	36.0	36.0	36.0	32.0	36.0
24	34.22208510440859	36.0	36.0	36.0	32.0	36.0
25	33.77272733131072	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	60.0
5	139.0
6	228.0
7	62.0
8	233.0
9	293.0
10	176.0
11	61.0
12	121.0
13	96.0
14	184.0
15	259.0
16	480.0
17	613.0
18	843.0
19	1040.0
20	1595.0
21	2135.0
22	3138.0
23	4521.0
24	6542.0
25	9172.0
26	12865.0
27	17897.0
28	24810.0
29	34033.0
30	46326.0
31	66190.0
32	99086.0
33	151211.0
34	364662.0
35	702709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.889588929504995	17.7384678047196	11.119410487891646	26.252532777883758
2	17.8135971582795	19.904738950440024	35.71857584022025	26.56308805106023
3	19.120831389249062	23.11131676157235	28.34623088245529	29.421620966723296
4	13.232480063626245	15.138689358652352	35.24398976256815	36.38484081515326
5	15.327992388250781	35.60607134983491	33.06604971617097	15.999886545743339
6	34.57935550088453	34.85464471573649	16.490339733968757	14.075660049410224
7	30.72649294322402	30.263306376988595	20.40084370500245	18.60935697478493
8	28.47964841039083	31.728072732713986	19.519892366315712	20.27238649057947
9	27.500332195054618	14.1903406708663	18.905381817900345	39.40394531617874
10	16.75983925067952	25.56434198650186	30.64430696694368	27.031511795874945
11	37.79891093963715	20.994412759038944	21.98340602338125	19.223270277942657
12	24.912744610297974	23.742951332495785	27.683623474845476	23.66068058236076
13	29.1160915735814	18.97732984154697	25.319644911392373	26.586933673479262
14	23.726128827872056	19.822541328952784	24.228812257965444	32.222517585209715
15	25.567371722830735	26.669857978041883	21.803215654801612	25.959554644325767
16	26.4035136387037	25.40414199580149	23.134185851772237	25.058158513722574
17	24.595192179274846	25.399743085668863	24.730875463649074	25.274189271407216
18	25.49840591408777	24.223137184304207	25.768893999255383	24.509562902352638
19	25.958059664221793	24.707618470576012	24.99617685944974	24.338145005752455
20	25.92589964030518	23.927673919705793	24.474990725357678	25.67143571463135
21	27.356873190488816	23.65012966214285	23.977979051449964	25.015018095918368
22	26.409709655184876	23.914466126438235	24.378883591013174	25.29694062736371
23	24.73718208082228	23.73185635180141	25.282338493863442	26.24862307351287
24	24.97333356563621	24.788846364556775	24.709669800819643	25.52815026898737
25	24.993563615035136	24.195000203254263	24.94329851340478	25.868137668305817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	107.0
2	339.0
3	571.0
4	571.0
5	571.0
6	1606.0
7	2641.0
8	2641.0
9	2641.0
10	2907.5
11	3174.0
12	3174.0
13	3174.0
14	3994.5
15	4815.0
16	4815.0
17	4815.0
18	7630.0
19	10445.0
20	10445.0
21	10445.0
22	16689.5
23	22934.0
24	22934.0
25	22934.0
26	33910.0
27	44886.0
28	44886.0
29	44886.0
30	53919.5
31	62953.0
32	62953.0
33	62953.0
34	78874.0
35	94795.0
36	94795.0
37	94795.0
38	106319.5
39	117844.0
40	117844.0
41	117844.0
42	133738.5
43	149633.0
44	149633.0
45	149633.0
46	170784.0
47	191935.0
48	191935.0
49	191935.0
50	196098.0
51	200261.0
52	200261.0
53	200261.0
54	187811.5
55	175362.0
56	175362.0
57	175362.0
58	163483.5
59	151605.0
60	151605.0
61	151605.0
62	137609.5
63	123614.0
64	123614.0
65	123614.0
66	104911.0
67	86208.0
68	86208.0
69	86208.0
70	66776.5
71	47345.0
72	47345.0
73	47345.0
74	38105.5
75	28866.0
76	28866.0
77	28866.0
78	23666.0
79	18466.0
80	18466.0
81	18466.0
82	13132.0
83	7798.0
84	7798.0
85	7798.0
86	5712.5
87	3627.0
88	3627.0
89	3627.0
90	2389.5
91	1152.0
92	1152.0
93	1152.0
94	655.0
95	158.0
96	158.0
97	158.0
98	375.5
99	593.0
100	593.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0085063165844819
2	0.003350973199947416
3	0.004639809046081037
4	0.014628286853616602
5	0.0323497797379539
6	0.0445937202762233
7	0.0650217684374412
8	0.08660976886017936
9	0.09627603770618151
10	0.11580190077510588
11	0.117799596336613
12	0.13062351300564254
13	0.12018394265196018
14	0.1228260561365341
15	0.11251536936746515
16	0.11045323201365136
17	0.11953952472889338
18	0.1286902592364421
19	0.12985021149796236
20	0.12005505906734684
21	0.128303608482602
22	0.14950495815150006
23	0.13796987732860416
24	0.1344900205440434
25	0.12920579357489553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1551788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.19783076882027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.80930946683175	40.24840562463234
2	10.900371446069357	10.725492594420018
3	2.919612535959698	4.309158103640142
4	1.2128828690780706	2.3868482454121644
5	0.7086400296748288	1.7431776127976997
6	0.4586044398010998	1.3537406171498476
7	0.3332419669274525	1.1476347325775926
8	0.22122342528511624	0.8706970111420723
9	0.17235959622035374	0.7631746421608041
>10	0.991101601595341	8.99717384307237
>50	0.12209771296663761	4.2548736146070905
>100	0.12714113079156347	13.347664157229556
>500	0.01792004299120929	5.930519838146185
>1k	0.005493735807524015	3.9214393630121864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2634	0.16973968093579794	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2273	0.14647619391308608	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2092	0.1348122295055768	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1977	0.12740142339030847	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1975	0.1272725398056951	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1914	0.12334159047498755	No Hit
GAACTACGACGGTATCTGATCGTCT	1909	0.12301938151345415	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1881	0.1212150113288671	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1875	0.120828360575027	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1732	0.11161318427517161	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1725	0.11116209172902483	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1683	0.10845553645214424	No Hit
TCGTAGTTCCGACCATAAACGATGC	1653	0.10652228268294381	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1628	0.10491123787527677	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1590	0.1024624497676229	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1575	0.10149582288302267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.444179230668107E-5
2	0.0	0.0	0.0	6.444179230668107E-5	6.444179230668107E-5
3	0.0	0.0	0.0	6.444179230668107E-5	6.444179230668107E-5
4	0.0	0.0	0.0	6.444179230668107E-5	6.444179230668107E-5
5	0.0	0.0	0.0	6.444179230668107E-5	6.444179230668107E-5
6	0.0	0.0	0.0	6.444179230668107E-5	6.444179230668107E-5
7	0.0	0.0	0.0	1.2888358461336213E-4	6.444179230668107E-5
8	0.0	0.0	0.0	1.2888358461336213E-4	6.444179230668107E-5
9	0.0	0.0	0.0	1.2888358461336213E-4	6.444179230668107E-5
10	6.444179230668107E-5	0.0	0.0	1.9332537692004321E-4	6.444179230668107E-5
11	6.444179230668107E-5	0.0	0.0	1.9332537692004321E-4	6.444179230668107E-5
12	6.444179230668107E-5	0.0	0.0	1.9332537692004321E-4	6.444179230668107E-5
13	6.444179230668107E-5	0.0	0.0	1.9332537692004321E-4	6.444179230668107E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	80	4.311005E-10	16.62644	13
CGTTATT	175	0.0	14.107313	2
ACGCTTA	100	1.0031727E-8	13.303728	17
TAGGTTG	50	0.0015003883	13.299007	5
CGGTCCA	375	0.0	13.173625	10
TTAATAC	60	4.1068712E-4	12.661225	3
GCGTTAT	195	0.0	12.657553	1
CGCATCG	275	0.0	12.437441	13
GTATCAA	2055	0.0	12.426574	1
CGACGCT	100	1.4401667E-7	12.351468	15
TCGACGC	100	1.4401667E-7	12.351468	14
AATCGTA	100	1.440767E-7	12.351069	13
AAGACGG	420	0.0	12.213373	5
CAAGACG	420	0.0	12.212192	4
GCATCGC	285	0.0	12.001427	14
GCAAGAC	460	0.0	11.973116	3
GCTCGTA	105	2.717734E-7	11.762545	9
ATAACGA	195	0.0	11.692566	12
CGACCAT	320	0.0	11.578381	10
ATCGCCA	305	0.0	11.527821	16
>>END_MODULE