FastQCFastQC Report
Thu 2 Feb 2017
SRR4062396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551788
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21910.1411919669439382No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20130.129721327913349No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19840.12785251593645525No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19340.12463042632112119No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17860.11509304105973238No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17670.11386864700590545No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17100.11019546484442462No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC16940.10916439616751772No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16500.10632895730602375No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16330.1052334468368102No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15740.10143138109071599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22250.022.4491841
TAGTACG1553.45608E-1119.870882
GGTATCA11550.018.8607061
GTATTAT1601.0986696E-917.8783781
TACCGTC5900.017.5253477
ATACCGT5850.017.2990686
ACCGTCG5900.017.1524688
CGGCTAG902.215955E-417.11103629
TTAGATC1702.4683686E-916.8234564
GTCGTAG6000.016.49992811
CGTCGTA6000.016.49992810
GTCCTAT5800.016.3134271
AACGCAG30500.016.3015676
TAGATCG1351.0859803E-616.2962255
GTACTAC1653.105015E-816.0030251
CCGTCGT6100.015.8687839
ATCGTTT6150.015.73976829
CATCGTT6350.015.59048328
GTATTAG5400.015.4844081
TGCGTAG1857.570634E-915.45939113
Produced by FastQC (version 0.11.5)