FastQCFastQC Report
Thu 2 Feb 2017
SRR4062395_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062395_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023382
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64050.3165492230335152No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43880.21686463554583366No Hit
TATCAACGCAGAGTACTTTTTTTTT42570.21039032669065952No Hit
GTACATGGAAGCAGTGGTATCAACG35420.17505345011470894No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32740.16180829917435263No Hit
TCCATGTACTCTGCGTTGATACCAC29260.14460937183389No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27630.13655355241867329No Hit
GGTATCAACGCAGAGTACTTTTTTT21350.10551640767783839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC905.4060183E-712.6669813
CGTAACA550.003064444512.09330415
CGCGCTA951.0368585E-612.00326816
CGCATCG2550.011.92186313
TAAATCC3000.011.3977434
TAAGCTG1201.2784403E-711.0816885
GCATCGC2750.011.0556414
GCACCGT1553.1286618E-1011.0328046
CGCCAGT2800.010.86036918
ATAACGA1158.808347E-710.74019412
ATCGCCA2950.010.63001316
CGTCTTA1701.382432E-1010.61975115
GTATTAG3250.010.5155291
GCGTTAT1451.4601028E-810.4752391
AACCGCG1002.398039E-510.4505187
TAATACC1002.4045983E-510.4479314
TAGGATA1002.4045983E-510.4479314
CGAACGA1106.027336E-610.36645916
ACGCGCG1106.0451694E-610.36389313
CGAGCCG2850.010.33538115
Produced by FastQC (version 0.11.5)