##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062395_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2023382
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17071912273609	32.0	32.0	32.0	32.0	32.0
2	30.887815548423383	32.0	32.0	32.0	32.0	32.0
3	30.880547518955886	32.0	32.0	32.0	32.0	32.0
4	30.931800816652515	32.0	32.0	32.0	32.0	32.0
5	30.777457247321564	32.0	32.0	32.0	32.0	32.0
6	34.419137859287076	36.0	36.0	36.0	32.0	36.0
7	34.29844685778563	36.0	36.0	36.0	32.0	36.0
8	34.29425387791331	36.0	36.0	36.0	32.0	36.0
9	34.41902072866122	36.0	36.0	36.0	32.0	36.0
10	34.15182353109793	36.0	36.0	36.0	32.0	36.0
11	34.43558210955717	36.0	36.0	36.0	32.0	36.0
12	34.23947084633549	36.0	36.0	36.0	32.0	36.0
13	34.33755514282523	36.0	36.0	36.0	32.0	36.0
14	34.26136340048493	36.0	36.0	36.0	32.0	36.0
15	34.22129286511395	36.0	36.0	36.0	32.0	36.0
16	34.21327411235249	36.0	36.0	36.0	32.0	36.0
17	34.14789199469008	36.0	36.0	36.0	32.0	36.0
18	34.158582511853915	36.0	36.0	36.0	32.0	36.0
19	34.14728113623626	36.0	36.0	36.0	32.0	36.0
20	34.12539994919398	36.0	36.0	36.0	32.0	36.0
21	34.09948541600153	36.0	36.0	36.0	32.0	36.0
22	34.06223738275818	36.0	36.0	36.0	32.0	36.0
23	34.031449325930545	36.0	36.0	36.0	32.0	36.0
24	34.00603741656296	36.0	36.0	36.0	32.0	36.0
25	33.57100339925926	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	81.0
5	196.0
6	353.0
7	80.0
8	306.0
9	349.0
10	248.0
11	75.0
12	168.0
13	128.0
14	458.0
15	603.0
16	835.0
17	1107.0
18	1524.0
19	1960.0
20	2900.0
21	3885.0
22	5891.0
23	8531.0
24	11450.0
25	16045.0
26	22034.0
27	28691.0
28	38583.0
29	51558.0
30	66762.0
31	91892.0
32	132998.0
33	194435.0
34	448531.0
35	890711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59443690959632	17.437049968564526	11.087677294097643	25.88083582774151
2	16.77625628550501	20.196648455547507	36.92209680592061	26.10499845302688
3	19.28893818168878	24.003501238580448	28.368039286625113	28.339521293105662
4	12.885584115602697	15.376554508575346	35.4680294819697	36.269831893852256
5	14.550281684221975	36.45960383753037	33.544425519923074	15.445688958324578
6	33.989343501968925	34.970293132808685	17.01064265328605	14.029720711936347
7	29.913041586032847	30.56323908840797	21.005825417494847	18.51789390806434
8	28.04135205939152	32.70931277107671	19.870021686136702	19.379313483395066
9	27.422343809519102	14.442542581663295	18.951672347519803	39.18344126129781
10	15.924843006457875	26.969241586724614	31.858741191612033	25.247174215205476
11	36.6105146901646	21.471846820099913	22.488648869043377	19.42898962069211
12	24.4825139040519	23.94966134391483	28.985506529138878	22.582318222894397
13	29.497411769828872	19.923854916598174	25.341596404336926	25.23713690923603
14	23.236819850130367	19.813462138940423	25.644106386358427	31.305611624570783
15	24.86515799312146	27.331130208815235	23.012870911384027	24.790840886679277
16	25.198358115315877	26.026672709604508	24.446239953450906	24.328729221628702
17	23.633104863870898	25.99098691187175	25.888306787428387	24.48760143682896
18	24.326598076913562	25.012000732415835	27.00189537340836	23.65950581726225
19	24.974698923374238	25.443554473403555	26.05206701754125	23.529679585680952
20	25.19121629746264	24.580343503990097	26.050743265677955	24.17769693286931
21	25.858542569706554	24.525404546199102	25.171856975802203	24.44419590829214
22	25.204534531427957	25.002091253905945	25.347630745441684	24.445743469224414
23	24.003347704441847	24.868781765206343	25.995093267025954	25.132777263325856
24	24.207339192559154	25.420178152270257	25.862069818784327	24.51041283638626
25	24.41535509739092	25.023717369131344	25.922973111407053	24.63795442207069
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	597.0
1	597.0
2	1146.0
3	1695.0
4	1695.0
5	1695.0
6	4305.5
7	6916.0
8	6916.0
9	6916.0
10	7095.5
11	7275.0
12	7275.0
13	7275.0
14	7197.0
15	7119.0
16	7119.0
17	7119.0
18	11667.5
19	16216.0
20	16216.0
21	16216.0
22	25134.5
23	34053.0
24	34053.0
25	34053.0
26	48671.5
27	63290.0
28	63290.0
29	63290.0
30	78081.5
31	92873.0
32	92873.0
33	92873.0
34	111058.0
35	129243.0
36	129243.0
37	129243.0
38	146203.0
39	163163.0
40	163163.0
41	163163.0
42	185370.5
43	207578.0
44	207578.0
45	207578.0
46	238530.0
47	269482.0
48	269482.0
49	269482.0
50	268554.5
51	267627.0
52	267627.0
53	267627.0
54	248833.5
55	230040.0
56	230040.0
57	230040.0
58	210012.5
59	189985.0
60	189985.0
61	189985.0
62	166436.5
63	142888.0
64	142888.0
65	142888.0
66	117623.5
67	92359.0
68	92359.0
69	92359.0
70	70627.5
71	48896.0
72	48896.0
73	48896.0
74	37483.0
75	26070.0
76	26070.0
77	26070.0
78	20578.5
79	15087.0
80	15087.0
81	15087.0
82	10613.5
83	6140.0
84	6140.0
85	6140.0
86	4482.5
87	2825.0
88	2825.0
89	2825.0
90	1882.5
91	940.0
92	940.0
93	940.0
94	569.5
95	199.0
96	199.0
97	199.0
98	512.5
99	826.0
100	826.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009390218950252598
2	0.003756087580101039
3	0.004843376090130287
4	0.015567994575418779
5	0.03592994303596651
6	0.0471487835712683
7	0.06825206510683597
8	0.08965187987241163
9	0.09746058826262169
10	0.12054075799824256
11	0.12049133579324121
12	0.13581221934365334
13	0.12370377911832763
14	0.12647142259840208
15	0.11431356016807503
16	0.11317684945304446
17	0.1256806673183808
18	0.13205613176355233
19	0.13428013098861213
20	0.12370377911832763
21	0.13151248750853767
22	0.15138021391907214
23	0.1429784390688461
24	0.13596048595865734
25	0.13398359775860416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2023382.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.39823128754389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3996974502807	42.64804874773835
2	13.115803030467593	14.269529735464904
3	3.777632538115568	6.164895855832863
4	1.5310248232757537	3.3314016977410184
5	0.8017827971522241	2.1807783020930285
6	0.4920107825708226	1.605870980775184
7	0.3260901069271597	1.2417107540042485
8	0.24549770671847285	1.0683712824506482
9	0.1834058485122986	0.897925839017423
>10	0.9595701410315454	9.454894309477796
>50	0.07989238111428995	3.046613112081855
>100	0.077942322056411	8.854308015482843
>500	0.007283073039378238	2.6464749051370933
>1k	0.0022759603248056984	2.2719798129264093
>5k	9.103841299222795E-5	0.3171966497764381
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6405	0.3165492230335152	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4388	0.21686463554583366	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4257	0.21039032669065952	No Hit
GTACATGGAAGCAGTGGTATCAACG	3542	0.17505345011470894	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3274	0.16180829917435263	No Hit
TCCATGTACTCTGCGTTGATACCAC	2926	0.14460937183389	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2763	0.13655355241867329	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2135	0.10551640767783839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.942220500132946E-5
2	0.0	0.0	0.0	0.0	4.942220500132946E-5
3	0.0	0.0	0.0	0.0	4.942220500132946E-5
4	0.0	0.0	0.0	0.0	9.884441000265892E-5
5	4.942220500132946E-5	4.942220500132946E-5	0.0	0.0	9.884441000265892E-5
6	4.942220500132946E-5	4.942220500132946E-5	0.0	0.0	9.884441000265892E-5
7	4.942220500132946E-5	4.942220500132946E-5	0.0	0.0	9.884441000265892E-5
8	4.942220500132946E-5	4.942220500132946E-5	0.0	4.942220500132946E-5	9.884441000265892E-5
9	4.942220500132946E-5	4.942220500132946E-5	0.0	9.884441000265892E-5	9.884441000265892E-5
10	4.942220500132946E-5	4.942220500132946E-5	0.0	9.884441000265892E-5	1.4826661500398836E-4
11	4.942220500132946E-5	4.942220500132946E-5	0.0	9.884441000265892E-5	1.4826661500398836E-4
12	4.942220500132946E-5	4.942220500132946E-5	0.0	9.884441000265892E-5	4.942220500132946E-4
13	4.942220500132946E-5	9.884441000265892E-5	0.0	9.884441000265892E-5	5.930664600159534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCGC	90	5.4060183E-7	12.66698	13
CGTAACA	55	0.0030644445	12.093304	15
CGCGCTA	95	1.0368585E-6	12.003268	16
CGCATCG	255	0.0	11.921863	13
TAAATCC	300	0.0	11.397743	4
TAAGCTG	120	1.2784403E-7	11.081688	5
GCATCGC	275	0.0	11.05564	14
GCACCGT	155	3.1286618E-10	11.032804	6
CGCCAGT	280	0.0	10.860369	18
ATAACGA	115	8.808347E-7	10.740194	12
ATCGCCA	295	0.0	10.630013	16
CGTCTTA	170	1.382432E-10	10.619751	15
GTATTAG	325	0.0	10.515529	1
GCGTTAT	145	1.4601028E-8	10.475239	1
AACCGCG	100	2.398039E-5	10.450518	7
TAATACC	100	2.4045983E-5	10.447931	4
TAGGATA	100	2.4045983E-5	10.447931	4
CGAACGA	110	6.027336E-6	10.366459	16
ACGCGCG	110	6.0451694E-6	10.363893	13
CGAGCCG	285	0.0	10.335381	15
>>END_MODULE