FastQCFastQC Report
Thu 2 Feb 2017
SRR4062395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023382
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG2050.018.24373835
TCGCCGA902.2164526E-417.11095635
GTACTAA1702.4629117E-916.8275381
CGCGAAC953.3479364E-416.2103812
TAGGACG5900.015.2883774
ACCGTCG3500.015.085588
TAACGGC2355.0931703E-1114.97858936
ATAACGC5600.014.9288063
GTCCTAA5050.014.8154091
TGTAGGA8050.014.7583612
GTATAGA1951.5126716E-814.670161
GTATAGT2102.5374902E-914.6701591
CATCGTT5550.014.66653328
GTATTAG5650.014.4105111
CGAGCCG5350.014.39239315
GTATTAT2451.0004442E-1014.3707681
TATATTG1851.2666169E-714.2704955
TAACGCC5400.014.2594834
TAATACT5250.014.2478444
TACGCTA4050.014.1233299
Produced by FastQC (version 0.11.5)