##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2023382
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249786248963368	32.0	32.0	32.0	32.0	32.0
2	31.43684089311855	32.0	32.0	32.0	32.0	32.0
3	31.511193635210752	32.0	32.0	32.0	32.0	32.0
4	31.609576441818696	32.0	32.0	32.0	32.0	32.0
5	31.55493821730153	32.0	32.0	32.0	32.0	32.0
6	35.14579451630982	36.0	36.0	36.0	36.0	36.0
7	35.15940094356874	36.0	36.0	36.0	36.0	36.0
8	35.104600614219166	36.0	36.0	36.0	36.0	36.0
9	35.21444591283307	36.0	36.0	36.0	36.0	36.0
10	35.10563650363599	36.0	36.0	36.0	36.0	36.0
11	35.227983149004984	36.0	36.0	36.0	36.0	36.0
12	35.14188521989421	36.0	36.0	36.0	36.0	36.0
13	35.180791862337415	36.0	36.0	36.0	36.0	36.0
14	35.14565218035942	36.0	36.0	36.0	36.0	36.0
15	35.11691366237319	36.0	36.0	36.0	36.0	36.0
16	35.12283543097645	36.0	36.0	36.0	36.0	36.0
17	35.103214321368874	36.0	36.0	36.0	36.0	36.0
18	35.109439542310845	36.0	36.0	36.0	36.0	36.0
19	35.09705680884776	36.0	36.0	36.0	36.0	36.0
20	35.07516820847472	36.0	36.0	36.0	36.0	36.0
21	35.06802917096228	36.0	36.0	36.0	36.0	36.0
22	35.05048577085296	36.0	36.0	36.0	36.0	36.0
23	35.018434976687544	36.0	36.0	36.0	36.0	36.0
24	34.99824748861065	36.0	36.0	36.0	36.0	36.0
25	34.97652346418027	36.0	36.0	36.0	36.0	36.0
26	34.93603135740063	36.0	36.0	36.0	32.0	36.0
27	34.91871628787841	36.0	36.0	36.0	32.0	36.0
28	34.90126431884834	36.0	36.0	36.0	32.0	36.0
29	34.875714521528806	36.0	36.0	36.0	32.0	36.0
30	34.8624293386024	36.0	36.0	36.0	32.0	36.0
31	34.85536542284156	36.0	36.0	36.0	32.0	36.0
32	34.81399112970264	36.0	36.0	36.0	32.0	36.0
33	34.78582195551804	36.0	36.0	36.0	32.0	36.0
34	34.771630369351904	36.0	36.0	36.0	32.0	36.0
35	34.73200166849364	36.0	36.0	36.0	32.0	36.0
36	34.70660903378601	36.0	36.0	36.0	32.0	36.0
37	34.69458807086353	36.0	36.0	36.0	32.0	36.0
38	34.65805122315015	36.0	36.0	36.0	32.0	36.0
39	34.636764091011976	36.0	36.0	36.0	32.0	36.0
40	34.618299955223485	36.0	36.0	36.0	32.0	36.0
41	34.592329574939384	36.0	36.0	36.0	32.0	36.0
42	34.55613868266101	36.0	36.0	36.0	32.0	36.0
43	34.54490205013191	36.0	36.0	36.0	32.0	36.0
44	34.509773735261064	36.0	36.0	36.0	32.0	36.0
45	34.454394177668874	36.0	36.0	36.0	32.0	36.0
46	34.45830446252858	36.0	36.0	36.0	32.0	36.0
47	34.41455049021885	36.0	36.0	36.0	32.0	36.0
48	34.37226238050946	36.0	36.0	36.0	32.0	36.0
49	34.35177144009386	36.0	36.0	36.0	32.0	36.0
50	33.840819479465566	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	19.0
21	62.0
22	203.0
23	581.0
24	1455.0
25	3538.0
26	6938.0
27	12927.0
28	21437.0
29	32578.0
30	45911.0
31	64600.0
32	93766.0
33	151359.0
34	329996.0
35	1258003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.925295086189045	18.1761921215114	11.595642823000682	26.302869969298875
2	16.241261117440203	20.704547307966994	36.55629335461885	26.497898219973955
3	18.75351209495074	24.33792222362253	28.619008433970144	28.289557247456585
4	12.630314256653971	15.804313380890767	35.57610095592618	35.989271406529085
5	14.315537056275089	36.66969460042642	33.48665748731579	15.528110855982707
6	34.186283279874665	35.17300345463262	16.755017619120576	13.885695646372142
7	29.851125418297393	30.658832913820977	20.99243790467539	18.49760376320624
8	28.099071850072647	32.8665401456968	19.70064940841562	19.33373859581492
9	27.494264553109595	14.266312540093764	18.810486601146	39.42893630565064
10	15.87614202360207	27.029547559482094	31.931044162693944	25.163266254221895
11	36.91052900539789	21.33561532127893	22.371554160311792	19.382301513011384
12	24.514056169324427	23.969670581234784	28.957606620993957	22.558666628446826
13	29.67022539490813	19.738536766660967	25.36614440575235	25.225093432678552
14	23.31067489974706	19.699641491324922	25.58548015154825	31.404203457379776
15	25.058145224184063	27.300381242889383	22.8326633329742	24.808810199952358
16	25.3196875330511	25.97468001593372	24.356300490960184	24.349331960054997
17	23.706991561652718	26.070905049071307	25.818110470489508	24.403992918786468
18	24.4684394741082	24.87518422126914	27.02000907391684	23.63636723070582
19	25.124865200935858	25.384628310422848	26.031070751840236	23.45943573680106
20	25.37499098044759	24.468933696158217	25.985256367804006	24.170818955590196
21	26.05869776443598	24.49710435300897	25.13969186243626	24.30450602011879
22	25.277826925414974	24.83762334546813	25.467558770415078	24.416990958701817
23	24.14754109703457	24.60331267155683	26.02835253056516	25.22079370084344
24	24.376514172805727	25.34370672468175	25.78732043677368	24.492458665738848
25	24.618584132902242	24.756027285010937	25.951846957223108	24.673541624863716
26	23.929193795338698	25.283213945760117	26.41176999696548	24.37582226193571
27	24.79477429373198	25.276887903519945	25.617703429209115	24.310634373538957
28	24.036489402396583	25.043664518118675	26.226634417030496	24.69321166245425
29	24.140276032899376	25.31815544469606	26.12225472006769	24.419313802336877
30	24.28518193796327	25.247926491389165	26.28040577607194	24.186485794575617
31	24.39435558881121	25.014110039527882	25.704538243396453	24.88699612826446
32	24.149221452004614	25.248717246669187	25.66949790005051	24.932563401275686
33	23.865933372936993	25.000469510947514	26.15348955362853	24.980107562486964
34	24.298871888748643	25.13895052936124	26.416119151005596	24.14605843088453
35	24.9368137109058	24.99083218097225	26.01327875803976	24.05907535008219
36	23.96181245063957	25.40711541369845	25.98841938892409	24.642652746737888
37	24.72083867504999	25.16212954350686	25.666334878930424	24.45069690251272
38	24.099601558183277	25.13035106569101	26.034085506345317	24.735961869780397
39	24.382049459765877	24.958905436541396	25.74239565242747	24.916649451265258
40	24.69632526136933	24.995724979267386	26.186898964209426	24.121050795153856
41	23.830991874001054	25.10890182872043	26.56463287703459	24.49547342024393
42	24.67878037859386	25.253016978504306	26.103474282167184	23.964728360734654
43	23.936656548293897	24.679324022848874	26.247490587541055	25.136528841316174
44	23.570487431439048	24.9144254520402	26.467172288771966	25.04791482774879
45	23.679068015826967	25.121405646585764	26.323551361038106	24.875974976549166
46	23.56239383487341	24.790981036196346	26.567512495175155	25.079112633755084
47	23.839393648851278	24.805054112372257	26.69644189777313	24.65911034100333
48	23.963789338839625	25.698508734386287	25.86639596477581	24.471305961998276
49	23.301383525206806	25.352256766146976	26.31939989581799	25.026959812828224
50	23.039940060749775	25.968996462358568	25.97962223643385	25.01144124045781
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	171.5
2	146.0
3	469.5
4	793.0
5	864.5
6	936.0
7	779.0
8	622.0
9	843.5
10	1065.0
11	1571.5
12	2078.0
13	3355.5
14	4633.0
15	5998.0
16	7363.0
17	7845.0
18	8327.0
19	8833.5
20	9340.0
21	10634.0
22	11928.0
23	12566.5
24	13205.0
25	14931.0
26	16657.0
27	19851.0
28	23045.0
29	26567.0
30	30089.0
31	33465.5
32	36842.0
33	42536.5
34	48231.0
35	54805.0
36	61379.0
37	70372.5
38	79366.0
39	84693.0
40	90020.0
41	97350.5
42	104681.0
43	108438.0
44	112195.0
45	122378.0
46	132561.0
47	140224.0
48	147887.0
49	149412.5
50	150938.0
51	146057.5
52	141177.0
53	138397.0
54	135617.0
55	135211.5
56	134806.0
57	130771.0
58	126736.0
59	116483.5
60	106231.0
61	94213.5
62	82196.0
63	71539.0
64	60882.0
65	52037.5
66	43193.0
67	37548.0
68	31903.0
69	28272.5
70	24642.0
71	19593.5
72	14545.0
73	12738.0
74	10931.0
75	8148.5
76	5366.0
77	4643.0
78	3920.0
79	3397.0
80	2874.0
81	2250.5
82	1627.0
83	1374.0
84	1121.0
85	893.5
86	666.0
87	484.0
88	302.0
89	187.5
90	73.0
91	49.5
92	26.0
93	18.0
94	10.0
95	8.5
96	7.0
97	5.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01625990544543739
2	0.004793953885128957
3	8.401774850226008E-4
4	1.9768882000531783E-4
5	0.0
6	5.930664600159534E-4
7	4.942220500132946E-5
8	9.884441000265892E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	4.942220500132946E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2023382.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.27940896883591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02935714548573	47.4409299174028
2	12.428334132899744	14.734886037310133
3	3.393338011595818	6.034652152766549
4	1.387403286595227	3.2897778732314213
5	0.7215309950007939	2.138596546817158
6	0.4414248334692378	1.570044193933394
7	0.3006252265008729	1.2474620023665923
8	0.2035113021768475	0.9651223769217352
9	0.16164497172129294	0.8623996547780196
>10	0.8000903189957415	8.637589620192504
>50	0.06357416665485063	2.6125422381723316
>100	0.06320747609248276	7.679789515461803
>500	0.004699372358209527	1.8316373046683796
>1k	0.001258760453091833	0.9545705659772254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	4.942220500132946E-5
4	0.0	0.0	0.0	0.0	4.942220500132946E-5
5	0.0	0.0	0.0	0.0	9.884441000265892E-5
6	0.0	0.0	0.0	0.0	9.884441000265892E-5
7	0.0	0.0	0.0	0.0	9.884441000265892E-5
8	0.0	0.0	0.0	0.0	9.884441000265892E-5
9	0.0	0.0	0.0	4.942220500132946E-5	9.884441000265892E-5
10	4.942220500132946E-5	0.0	0.0	4.942220500132946E-5	9.884441000265892E-5
11	4.942220500132946E-5	0.0	0.0	4.942220500132946E-5	9.884441000265892E-5
12	4.942220500132946E-5	4.942220500132946E-5	0.0	4.942220500132946E-5	1.9768882000531783E-4
13	9.884441000265892E-5	4.942220500132946E-5	0.0	4.942220500132946E-5	2.965332300079767E-4
14	9.884441000265892E-5	4.942220500132946E-5	0.0	9.884441000265892E-5	2.965332300079767E-4
15	9.884441000265892E-5	4.942220500132946E-5	0.0	1.9768882000531783E-4	6.42488665017283E-4
16	9.884441000265892E-5	4.942220500132946E-5	0.0	3.459554350093062E-4	7.907552800212713E-4
17	9.884441000265892E-5	4.942220500132946E-5	0.0	3.9537764001063567E-4	7.907552800212713E-4
18	9.884441000265892E-5	4.942220500132946E-5	0.0	3.9537764001063567E-4	7.907552800212713E-4
19	9.884441000265892E-5	4.942220500132946E-5	0.0	5.43644255014624E-4	7.907552800212713E-4
20	9.884441000265892E-5	4.942220500132946E-5	0.0	7.907552800212713E-4	8.895996900239303E-4
21	9.884441000265892E-5	4.942220500132946E-5	0.0	0.001284977330034566	9.884441000265892E-4
22	9.884441000265892E-5	4.942220500132946E-5	0.0	0.0020757326100558373	9.884441000265892E-4
23	9.884441000265892E-5	4.942220500132946E-5	0.0	0.004398576245118322	0.0010378663050279186
24	9.884441000265892E-5	4.942220500132946E-5	0.0	0.008253508235222019	0.0010378663050279186
25	9.884441000265892E-5	4.942220500132946E-5	0.0	0.010329240845277856	0.0010378663050279186
26	9.884441000265892E-5	4.942220500132946E-5	0.0	0.013096884325352305	0.0010378663050279186
27	9.884441000265892E-5	4.942220500132946E-5	0.0	0.018039104825485253	0.0010378663050279186
28	9.884441000265892E-5	4.942220500132946E-5	0.0	0.03118541135583889	0.0010378663050279186
29	9.884441000265892E-5	4.942220500132946E-5	0.0	0.05530344739648766	0.0010378663050279186
30	9.884441000265892E-5	4.942220500132946E-5	0.0	0.1041820081428025	0.0010378663050279186
31	9.884441000265892E-5	4.942220500132946E-5	0.0	0.19778766441532047	0.0010378663050279186
32	9.884441000265892E-5	4.942220500132946E-5	0.0	0.31150815812337956	0.001087288510029248
33	9.884441000265892E-5	4.942220500132946E-5	0.0	0.4263159403414679	0.001087288510029248
34	9.884441000265892E-5	4.942220500132946E-5	0.0	0.5552584731899365	0.0011367107150305775
35	9.884441000265892E-5	4.942220500132946E-5	0.0	0.7030308661439115	0.0011367107150305775
36	9.884441000265892E-5	4.942220500132946E-5	0.0	0.9141131037045896	0.001186132920031907
37	9.884441000265892E-5	4.942220500132946E-5	0.0	1.2172194869777433	0.001186132920031907
38	9.884441000265892E-5	4.942220500132946E-5	0.0	1.6037505522931408	0.001186132920031907
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	205	0.0	18.243738	35
TCGCCGA	90	2.2164526E-4	17.110956	35
GTACTAA	170	2.4629117E-9	16.827538	1
CGCGAAC	95	3.3479364E-4	16.21038	12
TAGGACG	590	0.0	15.288377	4
ACCGTCG	350	0.0	15.08558	8
TAACGGC	235	5.0931703E-11	14.978589	36
ATAACGC	560	0.0	14.928806	3
GTCCTAA	505	0.0	14.815409	1
TGTAGGA	805	0.0	14.758361	2
GTATAGA	195	1.5126716E-8	14.67016	1
GTATAGT	210	2.5374902E-9	14.670159	1
CATCGTT	555	0.0	14.666533	28
GTATTAG	565	0.0	14.410511	1
CGAGCCG	535	0.0	14.392393	15
GTATTAT	245	1.0004442E-10	14.370768	1
TATATTG	185	1.2666169E-7	14.270495	5
TAACGCC	540	0.0	14.259483	4
TAATACT	525	0.0	14.247844	4
TACGCTA	405	0.0	14.123329	9
>>END_MODULE