FastQCFastQC Report
Thu 2 Feb 2017
SRR4062394_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062394_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386271
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG70300.5071158525281132No Hit
TCCATGTACTCTGCGTTGATACCAC56100.4046827784754929No Hit
GTATCAACGCAGAGTACTTTTTTTT42890.30939116521949894No Hit
GAGTACATGGAAGCAGTGGTATCAA35200.2539186061022701No Hit
CATGTACTCTGCGTTGATACCACTG29090.20984352987258623No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28320.20428906036409908No Hit
TATCAACGCAGAGTACTTTTTTTTT26510.19123245022077212No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20580.14845582140865674No Hit
GCGTTGATACCACTGCTTCCATGTA19240.13878960174453625No Hit
GCTTCCATGTACTCTGCGTTGATAC18950.1366976586828982No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18060.1302775575626988No Hit
CCCATGTACTCTGCGTTGATACCAC17890.12905124611277302No Hit
GTACATGGGAAGCAGTGGTATCAAC17140.12364104853957127No Hit
CATGGAAGCAGTGGTATCAACGCAG15710.11332560516666655No Hit
GTATCAACGCAGAGTACATGGAAGC14600.10531851275832792No Hit
GGTATCAACGCAGAGTACTTTTTTT14220.10257734598790569No Hit
GCGCAAGACGGACCAGAGCGAAAGC13880.10012472308805422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCAA400.00527432614.25178810
GGGCCGT400.0052755914.2512746
TAATCGT400.0052755914.25127412
CGGACAT1650.013.2407385
AAGACGG1950.012.6650545
CTAGGCT905.416623E-712.6641394
GTCCTAA2800.012.5478951
CGCGTAA853.9369115E-612.29565910
TCGCGTA853.9385013E-612.2952169
CGAGATT853.9512415E-612.2916654
TTACCCT853.9512415E-612.2916654
TAACGAA701.0901932E-412.21493613
ATCGCCA1650.012.09242616
CGTTGAA550.003065273912.09242516
GAACCGC802.8632121E-511.8760616
TTATACT802.8716895E-511.8726314
CGTTATT802.8738123E-511.8717742
TTGCGCG1459.640644E-1111.79458218
ATTTGCG1459.640644E-1111.79458216
CGCAAGA2100.011.7587092
Produced by FastQC (version 0.11.5)