FastQCFastQC Report
Thu 2 Feb 2017
SRR4062394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386271
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25650.18502875700350077No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24950.17997923926851245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG6250.021.1205374
GTAGGAC7950.021.031983
CGTCTTA1057.749077E-820.95215815
AACGAAT2850.020.84188331
GTATCGT654.9330207E-420.30747610
CGCGTAA2250.019.5553510
TCGCGTA2200.018.9997969
TGTAGGA8750.018.607532
GGACGTG6500.018.2773886
AGGACGT6900.018.1743745
GCGTAAC2300.018.1737211
GATATAC5050.017.8650631
GTCCTAC7450.017.7217671
GTATTAG3000.017.6036221
GTCGCGT2500.017.5998128
GACGTGA3550.017.3519297
ACTGTTC6150.017.170558
CCAACGA3600.017.1109329
CGCAATA2450.017.0610436
CAACGAA3750.017.01315130
Produced by FastQC (version 0.11.5)