##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386271
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18899551386417	32.0	32.0	32.0	32.0	32.0
2	31.375692054439572	32.0	32.0	32.0	32.0	32.0
3	31.492654755094783	32.0	32.0	32.0	32.0	32.0
4	31.604636467184267	32.0	32.0	32.0	32.0	32.0
5	31.53070287122792	32.0	32.0	32.0	32.0	32.0
6	35.13508037028835	36.0	36.0	36.0	36.0	36.0
7	35.14838007864263	36.0	36.0	36.0	36.0	36.0
8	35.09185144895911	36.0	36.0	36.0	36.0	36.0
9	35.20897284874314	36.0	36.0	36.0	36.0	36.0
10	35.10443268307568	36.0	36.0	36.0	36.0	36.0
11	35.22798861117343	36.0	36.0	36.0	36.0	36.0
12	35.14289702374211	36.0	36.0	36.0	36.0	36.0
13	35.177103178238596	36.0	36.0	36.0	36.0	36.0
14	35.13708863562752	36.0	36.0	36.0	36.0	36.0
15	35.099425725561595	36.0	36.0	36.0	36.0	36.0
16	35.10767591618089	36.0	36.0	36.0	36.0	36.0
17	35.08323192218549	36.0	36.0	36.0	36.0	36.0
18	35.088497847823405	36.0	36.0	36.0	36.0	36.0
19	35.073292307204	36.0	36.0	36.0	36.0	36.0
20	35.05938304992314	36.0	36.0	36.0	36.0	36.0
21	35.03544400770124	36.0	36.0	36.0	36.0	36.0
22	35.01584105849434	36.0	36.0	36.0	36.0	36.0
23	34.97214036793672	36.0	36.0	36.0	36.0	36.0
24	34.945351233633254	36.0	36.0	36.0	32.0	36.0
25	34.91789195619038	36.0	36.0	36.0	32.0	36.0
26	34.86129046917955	36.0	36.0	36.0	32.0	36.0
27	34.840179156889235	36.0	36.0	36.0	32.0	36.0
28	34.80687903014634	36.0	36.0	36.0	32.0	36.0
29	34.76558479546929	36.0	36.0	36.0	32.0	36.0
30	34.7517036712158	36.0	36.0	36.0	32.0	36.0
31	34.73968004812912	36.0	36.0	36.0	32.0	36.0
32	34.704512321183955	36.0	36.0	36.0	32.0	36.0
33	34.66493275845776	36.0	36.0	36.0	32.0	36.0
34	34.65010881710719	36.0	36.0	36.0	32.0	36.0
35	34.59019051830414	36.0	36.0	36.0	32.0	36.0
36	34.55212436817909	36.0	36.0	36.0	32.0	36.0
37	34.51359943329984	36.0	36.0	36.0	32.0	36.0
38	34.45563818329894	36.0	36.0	36.0	32.0	36.0
39	34.38344883504019	36.0	36.0	36.0	32.0	36.0
40	34.35336957925254	36.0	36.0	36.0	32.0	36.0
41	34.32065014704917	36.0	36.0	36.0	32.0	36.0
42	34.24005767991973	36.0	36.0	36.0	32.0	36.0
43	34.24898017775745	36.0	36.0	36.0	32.0	36.0
44	34.20902767207855	36.0	36.0	36.0	32.0	36.0
45	34.098138098539174	36.0	36.0	36.0	32.0	36.0
46	34.142357446704146	36.0	36.0	36.0	32.0	36.0
47	34.071870507281766	36.0	36.0	36.0	32.0	36.0
48	34.04175301943127	36.0	36.0	36.0	32.0	36.0
49	34.036289441242005	36.0	36.0	36.0	32.0	36.0
50	33.50744623525992	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	2.0
21	27.0
22	130.0
23	451.0
24	1096.0
25	2716.0
26	5679.0
27	10642.0
28	17773.0
29	25776.0
30	35025.0
31	49627.0
32	72323.0
33	110808.0
34	238192.0
35	816001.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29160017199634	17.42148338186362	11.327228809964245	25.959687636175794
2	15.734067226223928	20.32729410661405	37.224889356990076	26.71374931017194
3	19.9750841648831	23.96998557997846	27.55637983159088	28.49855042354756
4	12.588276708799032	15.380597951619674	35.30678816331065	36.724337176270645
5	14.320288024491603	36.75176065863024	33.22892854283182	15.699022774046345
6	34.91679795479072	34.42302476296001	16.64545858508913	14.014718697160136
7	30.438442727607175	30.063191153238545	20.34279036551321	19.155575753641067
8	28.439893787001242	33.10716302945095	19.401617721210354	19.051325462337452
9	27.140003650079965	14.735791198113501	18.40830544677051	39.71589970503603
10	15.929352918729457	27.06058194970536	31.67641824722583	25.333646884339355
11	37.54287581576762	21.261932190747697	21.45374172870961	19.74145026477507
12	24.606588466468676	23.79578019016484	29.0013280231643	22.596303320202182
13	30.098804634880196	19.654454287797986	25.13996181121873	25.10677926610309
14	23.412161114241012	19.710503934656355	25.196155730012386	31.68117922109025
15	25.334801059821636	27.315438323386985	22.96325898760055	24.38650162919083
16	25.554094401455412	25.992681084722975	24.574632232802966	23.878592281018644
17	23.639100868444913	26.069145210424228	26.05421306512219	24.237540856008675
18	24.781301780099273	24.739246510963586	27.399043909884863	23.080407799052278
19	25.002903472697618	25.940815323987877	25.52927962858633	23.527001574728175
20	24.946276738098106	24.454309438774956	26.657918978323863	23.94149484480307
21	25.504104175879032	24.99460783641871	24.701086584080603	24.800201403621656
22	24.58602971569051	25.485709504130146	25.52689914165412	24.401361638525223
23	23.531690412624947	25.090620809351126	25.812124757713317	25.565564020310603
24	23.935579695456372	25.993041764561188	25.806209608366622	24.265168931615825
25	24.748912730627705	24.923193228452444	25.56873800288688	24.759156038032966
26	23.447868418224143	25.71077372317534	26.22892637875278	24.612431479847736
27	24.78786615315476	25.57436460836301	25.879716159394516	23.75805307908771
28	23.98809467990025	25.033849802816334	26.042743446266996	24.935312071016416
29	23.58932705077146	25.63192911054188	26.373775401779305	24.404968436907357
30	24.338098394902584	25.440408116450534	26.438409228787158	23.783084259859724
31	24.081510757997535	25.40946178633182	25.58965743350326	24.919370022167385
32	23.83422866091839	25.723181109609882	25.790411831452868	24.652178398018858
33	23.497570099929955	25.282502483280687	26.298104771722127	24.921822645067234
34	23.871522956189665	25.824964959953718	26.691750747148284	23.611761336708334
35	24.74393534886036	25.494293684279622	26.252082024366086	23.509688942493927
36	23.74160607846518	26.062436565433455	26.00754109405737	24.188416262044
37	24.66133966590948	25.64397581713821	25.804334073207908	23.890350443744403
38	24.07869745525947	25.650684462128975	25.545005269532435	24.725612813079117
39	24.316457604609777	25.266921114269863	25.968659807497957	24.4479614736224
40	24.960487523723714	25.471570854472176	25.625869689259893	23.942071932544216
41	23.607288906714487	25.547385756464646	26.61463739773825	24.230687939082618
42	25.112405871579224	25.967649903950957	25.883827909550156	23.036116314919667
43	24.238406487620388	24.800850627330444	25.9810671939325	24.97967569111667
44	23.535369346974726	25.586844130765197	26.49301615629267	24.384770365967405
45	24.03101558064765	25.825397775759573	25.762567347942788	24.381019295649985
46	23.713197899970133	25.16825775139836	26.26191510143075	24.856629247200754
47	24.29510535818754	25.031757859754695	26.661814320576564	24.011322461481196
48	24.18221256882673	26.53774045623114	25.117238981411283	24.162807993530848
49	23.46200706788211	25.59744811800867	26.51725384142062	24.4232909726886
50	23.344947232501607	26.93227149112366	24.883319988169692	24.83946128820504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	30.0
2	29.0
3	152.0
4	275.0
5	294.0
6	313.0
7	298.0
8	283.0
9	378.0
10	473.0
11	731.0
12	989.0
13	1712.5
14	2436.0
15	3248.0
16	4060.0
17	4789.5
18	5519.0
19	5892.0
20	6265.0
21	7081.0
22	7897.0
23	8402.5
24	8908.0
25	9388.0
26	9868.0
27	12265.5
28	14663.0
29	17061.5
30	19460.0
31	22282.5
32	25105.0
33	29072.5
34	33040.0
35	38398.5
36	43757.0
37	49359.0
38	54961.0
39	58490.5
40	62020.0
41	66424.0
42	70828.0
43	74436.0
44	78044.0
45	86848.0
46	95652.0
47	103681.0
48	111710.0
49	112037.5
50	112365.0
51	104995.5
52	97626.0
53	94819.5
54	92013.0
55	92002.0
56	91991.0
57	88412.5
58	84834.0
59	77272.5
60	69711.0
61	61635.5
62	53560.0
63	46308.0
64	39056.0
65	33180.5
66	27305.0
67	23618.0
68	19931.0
69	17947.0
70	15963.0
71	12429.5
72	8896.0
73	7850.5
74	6805.0
75	4953.5
76	3102.0
77	2747.0
78	2392.0
79	2103.5
80	1815.0
81	1389.5
82	964.0
83	814.5
84	665.0
85	543.0
86	421.0
87	307.0
88	193.0
89	122.0
90	51.0
91	32.0
92	13.0
93	8.5
94	4.0
95	4.5
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01406651369032462
2	0.00403961418799066
3	2.885438705707614E-4
4	3.6067983821345175E-4
5	0.0
6	5.049517734988325E-4
7	7.213596764269035E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.6067983821345175E-4
47	0.0
48	0.0
49	0.0
50	7.213596764269035E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1386271.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.067900124868444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8588088638505	43.861708279585734
2	14.10253964548223	16.09604647990755
3	4.364923073503681	7.472909820343254
4	1.7430619148820339	3.978915330797994
5	0.8853904309084566	2.5263686341299016
6	0.47410635837525916	1.6233752584994623
7	0.2931906195090516	1.1712241094183602
8	0.21494791860569487	0.9813301080830515
9	0.14355164098051673	0.737297163921339
>10	0.7741320591339709	8.13263251567249
>50	0.07257385516666497	2.893071070685376
>100	0.06616942479537015	7.496612493283515
>500	0.005461161090066073	2.067662970234649
>1k	0.0011430337165254571	0.9608457654374909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2565	0.18502875700350077	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2495	0.17997923926851245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.442719352853807E-4
2	0.0	0.0	0.0	0.0	1.442719352853807E-4
3	0.0	0.0	0.0	0.0	1.442719352853807E-4
4	0.0	0.0	0.0	0.0	1.442719352853807E-4
5	0.0	0.0	0.0	0.0	1.442719352853807E-4
6	0.0	0.0	0.0	0.0	1.442719352853807E-4
7	0.0	0.0	0.0	0.0	1.442719352853807E-4
8	0.0	0.0	0.0	0.0	1.442719352853807E-4
9	0.0	0.0	0.0	0.0	1.442719352853807E-4
10	0.0	0.0	0.0	0.0	1.442719352853807E-4
11	0.0	0.0	0.0	0.0	1.442719352853807E-4
12	0.0	0.0	0.0	7.213596764269035E-5	2.885438705707614E-4
13	0.0	0.0	0.0	7.213596764269035E-5	3.6067983821345175E-4
14	0.0	0.0	0.0	7.213596764269035E-5	3.6067983821345175E-4
15	0.0	0.0	0.0	2.1640790292807107E-4	3.6067983821345175E-4
16	0.0	0.0	0.0	3.6067983821345175E-4	3.6067983821345175E-4
17	0.0	0.0	0.0	3.6067983821345175E-4	4.3281580585614215E-4
18	0.0	0.0	0.0	7.213596764269035E-4	4.3281580585614215E-4
19	0.0	0.0	0.0	0.0011541754822830457	5.049517734988325E-4
20	0.0	0.0	0.0	0.0015869912881391877	5.770877411415228E-4
21	0.0	0.0	0.0	0.0023804869322087818	5.770877411415228E-4
22	0.0	0.0	0.0	0.004256022090918731	5.770877411415228E-4
23	0.0	0.0	0.0	0.006997188861340964	5.770877411415228E-4
24	0.0	0.0	0.0	0.011469618855187767	5.770877411415228E-4
25	0.0	0.0	0.0	0.015004281269679594	5.770877411415228E-4
26	0.0	0.0	0.0	0.020198070939953298	5.770877411415228E-4
27	7.213596764269035E-5	0.0	0.0	0.025680404480797767	5.770877411415228E-4
28	7.213596764269035E-5	0.0	0.0	0.036284391724273245	5.770877411415228E-4
29	7.213596764269035E-5	0.0	0.0	0.05208216863802244	5.770877411415228E-4
30	7.213596764269035E-5	0.0	0.0	0.08093655569509858	5.770877411415228E-4
31	7.213596764269035E-5	0.0	0.0	0.1301332856274134	6.492237087842132E-4
32	7.213596764269035E-5	0.0	0.0	0.18827487554742184	6.492237087842132E-4
33	7.213596764269035E-5	0.0	0.0	0.24158335563537	7.213596764269035E-4
34	7.213596764269035E-5	0.0	0.0	0.31011252489592583	7.213596764269035E-4
35	7.213596764269035E-5	0.0	0.0	0.3919147122027367	7.213596764269035E-4
36	7.213596764269035E-5	0.0	0.0	0.49910876011977456	7.213596764269035E-4
37	7.213596764269035E-5	0.0	0.0	0.6490073008812851	7.213596764269035E-4
38	1.442719352853807E-4	0.0	0.0	0.8294193559556537	7.213596764269035E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	625	0.0	21.120537	4
GTAGGAC	795	0.0	21.03198	3
CGTCTTA	105	7.749077E-8	20.952158	15
AACGAAT	285	0.0	20.841883	31
GTATCGT	65	4.9330207E-4	20.307476	10
CGCGTAA	225	0.0	19.55535	10
TCGCGTA	220	0.0	18.999796	9
TGTAGGA	875	0.0	18.60753	2
GGACGTG	650	0.0	18.277388	6
AGGACGT	690	0.0	18.174374	5
GCGTAAC	230	0.0	18.17372	11
GATATAC	505	0.0	17.865063	1
GTCCTAC	745	0.0	17.721767	1
GTATTAG	300	0.0	17.603622	1
GTCGCGT	250	0.0	17.599812	8
GACGTGA	355	0.0	17.351929	7
ACTGTTC	615	0.0	17.17055	8
CCAACGA	360	0.0	17.11093	29
CGCAATA	245	0.0	17.06104	36
CAACGAA	375	0.0	17.013151	30
>>END_MODULE