FastQCFastQC Report
Thu 2 Feb 2017
SRR4062392_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062392_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871140
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24690.13195164445204527No Hit
GCGCAAGACGGACCAGAGCGAAAGC21440.11458255395106727No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19610.10480241991513196No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19190.10255779898885173No Hit
GAACTACGACGGTATCTGATCGTCT19170.10245091227807648No Hit
GGGTAGGCACACGCTGAGCCAGTCA19000.10154237523648685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19650.014.2041561
CGCATCG2850.014.0014213
ATCGCCA3050.013.70704216
TGGACGG655.4566684E-513.1530685
CTTACAC1251.03682396E-1012.9154343
ATTAGAC751.484347E-512.66218953
CGAACGA1750.012.48764916
TAACGAA1750.012.4869813
ACCGTCG2900.012.4492058
GCGTTAT1302.0008883E-1012.41471
TCGCCAG3300.012.381700517
ACGGTAT2850.012.3352449
CCGTCGT2950.012.2391849
ATAACGA1800.012.14044612
ACGAACG1800.012.13979515
CGCCAGT3400.012.01753318
CCGCATC3350.011.91197512
ACGCGCG1052.7172246E-711.76309813
AAGACGG3400.011.73465
GACGAGT658.019731E-411.693186
Produced by FastQC (version 0.11.5)