FastQCFastQC Report
Thu 2 Feb 2017
SRR4062392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871140
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21050.1124982630909499No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20020.10699359748602456No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19820.10592473037827208No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19340.10335944931966609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.022.6801071
TATACCG803.617566E-621.9998115
TAGGACG5650.020.2476164
AGGACGT5750.019.1302745
TTAGGAC4400.019.0003453
ATACCGT5300.018.6790876
GGACGTG6050.018.5452986
TACCGTC5600.017.6784217
ACCGTCG5150.017.5144148
GGCGTTA6500.016.92293442
TATGGCG7150.016.92293416
AACGAAT2251.8189894E-1216.62208231
AACGCAG32700.016.550326
GCGAGAA4450.016.31446820
CGCAATA5950.016.2687736
GACGTGA3400.016.1763347
GATATAC3950.016.1556471
GGTATCA13900.016.1476331
CCGTCGT5450.016.1466529
TATAACA4500.016.1331964
Produced by FastQC (version 0.11.5)