##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062392_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1871140 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33134826896972 32.0 32.0 32.0 32.0 32.0 2 31.408409846403796 32.0 32.0 32.0 32.0 32.0 3 31.502422052866166 32.0 32.0 32.0 32.0 32.0 4 31.603789668330535 32.0 32.0 32.0 32.0 32.0 5 31.534846671013394 32.0 32.0 32.0 32.0 32.0 6 35.135759483523415 36.0 36.0 36.0 36.0 36.0 7 35.1558050172622 36.0 36.0 36.0 36.0 36.0 8 35.094717124319935 36.0 36.0 36.0 36.0 36.0 9 35.23440309116368 36.0 36.0 36.0 36.0 36.0 10 35.09984501426938 36.0 36.0 36.0 36.0 36.0 11 35.243858289598855 36.0 36.0 36.0 36.0 36.0 12 35.15084921491711 36.0 36.0 36.0 36.0 36.0 13 35.203799288134505 36.0 36.0 36.0 36.0 36.0 14 35.159472300308906 36.0 36.0 36.0 36.0 36.0 15 35.121404598266295 36.0 36.0 36.0 36.0 36.0 16 35.14007717220518 36.0 36.0 36.0 36.0 36.0 17 35.104226300544056 36.0 36.0 36.0 36.0 36.0 18 35.12324625629296 36.0 36.0 36.0 36.0 36.0 19 35.11194993426467 36.0 36.0 36.0 36.0 36.0 20 35.10014269375888 36.0 36.0 36.0 36.0 36.0 21 35.10701283709396 36.0 36.0 36.0 36.0 36.0 22 35.08709075750612 36.0 36.0 36.0 36.0 36.0 23 35.05310399008091 36.0 36.0 36.0 36.0 36.0 24 35.02154034438898 36.0 36.0 36.0 36.0 36.0 25 35.00143121305728 36.0 36.0 36.0 36.0 36.0 26 34.96565462766014 36.0 36.0 36.0 32.0 36.0 27 34.948245454642624 36.0 36.0 36.0 32.0 36.0 28 34.943278963626454 36.0 36.0 36.0 32.0 36.0 29 34.91763684171147 36.0 36.0 36.0 32.0 36.0 30 34.90430967217846 36.0 36.0 36.0 32.0 36.0 31 34.90471049734386 36.0 36.0 36.0 32.0 36.0 32 34.87341353399532 36.0 36.0 36.0 32.0 36.0 33 34.85951558942676 36.0 36.0 36.0 32.0 36.0 34 34.85660185769103 36.0 36.0 36.0 32.0 36.0 35 34.842179633806126 36.0 36.0 36.0 32.0 36.0 36 34.81827281764058 36.0 36.0 36.0 32.0 36.0 37 34.81495665743878 36.0 36.0 36.0 32.0 36.0 38 34.78197622839552 36.0 36.0 36.0 32.0 36.0 39 34.77571640817897 36.0 36.0 36.0 32.0 36.0 40 34.77876588603739 36.0 36.0 36.0 32.0 36.0 41 34.75648374787563 36.0 36.0 36.0 32.0 36.0 42 34.739252006798 36.0 36.0 36.0 32.0 36.0 43 34.73715916500102 36.0 36.0 36.0 32.0 36.0 44 34.705278600211635 36.0 36.0 36.0 32.0 36.0 45 34.68298310121103 36.0 36.0 36.0 32.0 36.0 46 34.682726573105164 36.0 36.0 36.0 32.0 36.0 47 34.67372831535855 36.0 36.0 36.0 32.0 36.0 48 34.63046485030516 36.0 36.0 36.0 32.0 36.0 49 34.62324785959362 36.0 36.0 36.0 32.0 36.0 50 34.113300447855316 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 5.0 21 31.0 22 111.0 23 333.0 24 857.0 25 2105.0 26 4514.0 27 8428.0 28 14802.0 29 24221.0 30 36231.0 31 55504.0 32 84989.0 33 146277.0 34 334402.0 35 1158327.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74844656587496 18.11391048477835 11.53937638138712 26.598266567959573 2 16.53319465926692 20.173923360843673 36.43876659893301 26.8541153809564 3 18.78188242097168 23.585923608850894 28.471038670153327 29.161155300024106 4 12.546181862880015 15.803616521523034 35.77112287461888 35.87907874097807 5 14.621300383723293 36.49171093557938 33.37558921299315 15.511399467704182 6 34.796246118655574 35.27852153511514 16.48447729446912 13.440755051760167 7 30.580037079019785 30.121973835188086 20.601248758109367 18.696740327682765 8 28.193774917964447 33.0609681798262 19.22491101681328 19.52034588539607 9 27.329221757858846 14.129568070801756 18.55280737945851 39.98840279188089 10 16.144382568915205 26.70313284949282 31.308186453178276 25.844298128413694 11 37.983047767671046 20.9431683358808 21.95426317645927 19.119520719988884 12 24.641982962258304 23.619825347114592 28.542172151736374 23.19601953889073 13 29.577102728817728 19.32955310666225 25.23915901535962 25.8541851491604 14 23.40824310313499 19.805519629744435 24.760253107731113 32.025984159389466 15 25.216231815898325 27.600126126318713 21.764753038254753 25.4188890195282 16 25.923447737742766 25.823294889746357 23.64307320670821 24.610184165802664 17 24.258954434195196 25.942206355483822 24.876919952542302 24.92191925777868 18 25.102931902476566 24.989685432410187 25.883151447780495 24.024231217332748 19 25.797535192449523 24.94912192567098 24.979691524952703 24.27365135692679 20 25.76076616394284 24.65935205275928 24.549900060925424 25.029981722372458 21 26.98200027790545 24.33441645200252 23.98120931624571 24.70237395384632 22 26.071218615389547 24.559840525027525 24.542257661104994 24.826683198477934 23 24.65486281090672 24.458832583344915 25.20458116442383 25.68172344132454 24 25.018384514253345 25.10875722821382 24.888089613818316 24.984768643714528 25 25.02624068749532 24.701411973449343 25.021484228865827 25.25086311018951 26 24.640967538505937 25.62817319922614 25.34283912481161 24.38802013745631 27 25.47340124202358 24.88183674123796 24.63669207007493 25.00806994666353 28 24.689921652041 24.851480915377792 25.316865654093228 25.14173177848798 29 24.714559038874697 24.878897356691642 25.095396389366908 25.311147215066747 30 24.402289513344805 25.192449522750838 25.622187543422726 24.78307342048163 31 25.27475229004778 24.853030772684033 24.541295680708018 25.330921256560174 32 24.864040103893885 25.02901974197548 24.46636809645457 25.64057205767607 33 24.55321354895946 24.794296525113033 24.991983496691855 25.660506429235653 34 25.258879613497655 24.894342486398667 25.33904464657909 24.507733253524588 35 25.744145280417285 24.653847387154354 25.19838173519886 24.403625597229496 36 24.510298534583193 25.282501576578987 24.90054191562363 25.306657973214193 37 25.590121530190153 24.9779813375803 24.572827260386713 24.859069871842834 38 24.846296909905192 24.779118612182945 25.11388779033103 25.26069668758083 39 25.467789689707875 24.58244706435649 24.61707835864767 25.332684887287964 40 25.749970606154537 24.629797877229926 25.065574997060615 24.554656519554925 41 24.644601686672296 25.147824321002172 25.525508513526514 24.68206547879902 42 25.729875904528786 25.360101328601814 24.973545539083126 23.93647722778627 43 24.902946866616073 24.44445632074564 25.20206932672061 25.450527485917675 44 24.448678345821264 25.02816464828928 25.02249965261819 25.500657353271265 45 24.730752375557145 25.143655739281932 25.077065318468954 25.048526566691965 46 24.508494558661866 24.71665653914541 25.259961980376662 25.51488692181607 47 24.93421122951783 24.537982192673987 25.47420289235439 25.05360368545379 48 25.516423143110618 25.168453456181794 24.594364932607927 24.72075846809966 49 24.589929134110754 25.269194181087467 24.910963369924215 25.22991331487756 50 24.55429553870664 25.383790300987798 24.862663864095612 25.199250296209957 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10.0 1 10.0 2 10.0 3 28.5 4 47.0 5 61.5 6 76.0 7 89.0 8 102.0 9 147.0 10 192.0 11 305.0 12 418.0 13 644.0 14 870.0 15 1194.0 16 1518.0 17 1937.5 18 2357.0 19 2815.0 20 3273.0 21 4329.0 22 5385.0 23 6249.5 24 7114.0 25 9402.5 26 11691.0 27 14991.0 28 18291.0 29 21416.5 30 24542.0 31 27311.0 32 30080.0 33 35130.5 34 40181.0 35 47583.5 36 54986.0 37 64392.5 38 73799.0 39 78961.0 40 84123.0 41 90575.5 42 97028.0 43 99117.5 44 101207.0 45 111052.0 46 120897.0 47 129076.0 48 137255.0 49 142160.5 50 147066.0 51 141151.5 52 135237.0 53 132847.5 54 130458.0 55 132842.0 56 135226.0 57 131951.5 58 128677.0 59 117244.5 60 105812.0 61 92374.0 62 78936.0 63 68602.5 64 58269.0 65 50315.0 66 42361.0 67 36313.5 68 30266.0 69 27375.5 70 24485.0 71 18870.5 72 13256.0 73 11724.0 74 10192.0 75 7504.0 76 4816.0 77 4314.5 78 3813.0 79 3221.5 80 2630.0 81 2138.5 82 1647.0 83 1442.5 84 1238.0 85 1007.0 86 776.0 87 574.0 88 372.0 89 235.0 90 98.0 91 65.5 92 33.0 93 22.0 94 11.0 95 8.0 96 5.0 97 3.5 98 2.0 99 4.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015231356285473027 2 0.004596128563335721 3 3.741034877133726E-4 4 2.672167769381233E-4 5 0.0 6 5.344335538762466E-4 7 5.344335538762465E-5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 5.344335538762465E-5 47 0.0 48 0.0 49 0.0 50 5.344335538762465E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1871140.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.511026056450596 #Duplication Level Percentage of deduplicated Percentage of total 1 80.5105721677177 45.497350415896406 2 12.40304427698797 14.018175166323555 3 3.332184345414476 5.649154691058427 4 1.2850025632625601 2.904672533405454 5 0.6330485099653805 1.788711042082542 6 0.3996345796491023 1.355025608616546 7 0.2548252925820297 1.008030712426201 8 0.18487379680989804 0.8357926358943282 9 0.13426608973229132 0.6828763045823323 >10 0.6912087606630363 7.251083030945223 >50 0.07955646131824023 3.2150699678265093 >100 0.07848211556645623 9.372826564056844 >500 0.00997578024822324 3.7464117656397433 >1k 0.0033252600827410805 2.674819561245819 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2105 0.1124982630909499 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 2002 0.10699359748602456 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 1982 0.10592473037827208 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 1934 0.10335944931966609 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0688671077524932E-4 2 0.0 0.0 0.0 0.0 1.0688671077524932E-4 3 0.0 0.0 0.0 0.0 1.0688671077524932E-4 4 0.0 0.0 0.0 0.0 1.0688671077524932E-4 5 0.0 0.0 0.0 0.0 1.0688671077524932E-4 6 0.0 0.0 0.0 0.0 1.0688671077524932E-4 7 0.0 0.0 0.0 0.0 1.0688671077524932E-4 8 0.0 0.0 0.0 0.0 1.6033006616287396E-4 9 0.0 0.0 0.0 0.0 1.6033006616287396E-4 10 0.0 0.0 0.0 0.0 2.1377342155049864E-4 11 0.0 0.0 0.0 0.0 2.1377342155049864E-4 12 0.0 0.0 0.0 0.0 2.672167769381233E-4 13 0.0 0.0 0.0 0.0 3.741034877133726E-4 14 0.0 0.0 0.0 0.0 3.741034877133726E-4 15 0.0 0.0 0.0 0.0 5.344335538762466E-4 16 0.0 0.0 0.0 0.0 5.344335538762466E-4 17 0.0 0.0 0.0 0.0 5.344335538762466E-4 18 0.0 0.0 0.0 5.344335538762466E-5 5.878769092638712E-4 19 0.0 0.0 0.0 1.0688671077524932E-4 5.878769092638712E-4 20 0.0 5.344335538762466E-5 0.0 2.1377342155049864E-4 5.878769092638712E-4 21 0.0 5.344335538762466E-5 0.0 2.1377342155049864E-4 6.947636200391206E-4 22 0.0 5.344335538762466E-5 0.0 2.672167769381233E-4 7.482069754267452E-4 23 0.0 5.344335538762466E-5 0.0 3.206601323257479E-4 8.016503308143698E-4 24 0.0 5.344335538762466E-5 0.0 5.878769092638712E-4 8.016503308143698E-4 25 0.0 5.344335538762466E-5 0.0 6.947636200391206E-4 8.016503308143698E-4 26 0.0 5.344335538762466E-5 0.0 0.0010688671077524932 8.016503308143698E-4 27 0.0 5.344335538762466E-5 0.0 0.0012291971739153671 8.016503308143698E-4 28 0.0 5.344335538762466E-5 0.0 0.0024583943478307343 8.016503308143698E-4 29 0.0 5.344335538762466E-5 0.0 0.005611552315700589 8.016503308143698E-4 30 0.0 5.344335538762466E-5 0.0 0.012078198317603173 8.016503308143698E-4 31 0.0 5.344335538762466E-5 0.0 0.02939384546319356 9.085370415896192E-4 32 0.0 5.344335538762466E-5 0.0 0.051679724659833046 9.085370415896192E-4 33 0.0 5.344335538762466E-5 0.0 0.07733253524589288 9.085370415896192E-4 34 0.0 5.344335538762466E-5 0.0 0.11308614000021378 9.619803969772438E-4 35 0.0 5.344335538762466E-5 0.0 0.15119125239159015 9.619803969772438E-4 36 0.0 5.344335538762466E-5 0.0 0.2046346077792148 9.619803969772438E-4 37 0.0 5.344335538762466E-5 0.0 0.29425911476426136 9.619803969772438E-4 38 0.0 5.344335538762466E-5 0.0 0.4128499203694005 9.619803969772438E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2445 0.0 22.680107 1 TATACCG 80 3.617566E-6 21.999811 5 TAGGACG 565 0.0 20.247616 4 AGGACGT 575 0.0 19.130274 5 TTAGGAC 440 0.0 19.000345 3 ATACCGT 530 0.0 18.679087 6 GGACGTG 605 0.0 18.545298 6 TACCGTC 560 0.0 17.678421 7 ACCGTCG 515 0.0 17.514414 8 GGCGTTA 650 0.0 16.922934 42 TATGGCG 715 0.0 16.922934 16 AACGAAT 225 1.8189894E-12 16.622082 31 AACGCAG 3270 0.0 16.55032 6 GCGAGAA 445 0.0 16.314468 20 CGCAATA 595 0.0 16.26877 36 GACGTGA 340 0.0 16.176334 7 GATATAC 395 0.0 16.155647 1 GGTATCA 1390 0.0 16.147633 1 CCGTCGT 545 0.0 16.146652 9 TATAACA 450 0.0 16.133196 4 >>END_MODULE