FastQCFastQC Report
Thu 2 Feb 2017
SRR4062391_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062391_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752409
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG38550.5123543179308063No Hit
TCCATGTACTCTGCGTTGATACCAC29960.39818768781340996No Hit
GAGTACATGGAAGCAGTGGTATCAA17600.23391533062470013No Hit
CATGTACTCTGCGTTGATACCACTG15340.20387847566948297No Hit
GTATCAACGCAGAGTACTTTTTTTT12730.16918989538934276No Hit
GCTTCCATGTACTCTGCGTTGATAC10640.14141244987765964No Hit
GTCCTACAGTGGACATTTCTAAATT10180.13529875373633224No Hit
CCCATGTACTCTGCGTTGATACCAC9230.12267264214011263No Hit
TATCAACGCAGAGTACTTTTTTTTT8890.11815382325304456No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8880.1180209168151896No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8770.11655894599878523No Hit
GTACATGGGAAGCAGTGGTATCAAC8620.11456534943096108No Hit
GTCCTAAAGTGTGTATTTCTCATTT8460.112438846425282No Hit
GTCCTACAGTGTGCATTTCTCATTT8450.11230593998742705No Hit
CATGGAAGCAGTGGTATCAACGCAG8070.10725549534893922No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7960.10579352453253482No Hit
GCGCAAGACGGACCAGAGCGAAAGC7950.10566061809467989No Hit
GAATAGGACCGCGGTTCTATTTTGT7940.10552771165682495No Hit
GCGTTGATACCACTGCTTCCATGTA7910.10512899234326012No Hit
CTGTAGGACGTGGAATATGGCAAGA7900.10499608590540518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT250.006044996418.9914174
CGGTTCT1200.016.62744712
GCTCGTT350.002166665616.2881112
TCGGCGT902.2755557E-914.77995213
AACTGCC456.749042E-414.7799527
CCAACGA602.5596137E-514.25304619
ACGGTAT1203.6379788E-1214.2530469
CGGTCCA1550.014.09978710
CCGACCA759.63033E-713.9363129
CGGCGTC1001.0018994E-813.30195614
CGACCAT655.43427E-513.15665710
CGTCTTA655.43427E-513.15665715
AACCGCG655.4376662E-513.1557827
GGGTCTT655.4376662E-513.1557826
GGTCCAA1900.013.00277811
AGAATTT1850.012.84058117
GCGGTCG1051.9812433E-812.6693749
ATCGCCA1352.7284841E-1112.669373516
TACCGTC1051.9830622E-812.66852957
GTCGGCG1051.9830622E-812.668529512
Produced by FastQC (version 0.11.5)