FastQCFastQC Report
Thu 2 Feb 2017
SRR4062391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752409
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA13910.18487285505622608No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11190.1487223039596815No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8650.11496406874452592No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8000.10632515028395462No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7710.10247086358616125No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7700.10233795714830632No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG7540.10021145414262722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA353.2168883E-431.42828644
GTACCGT454.0677776E-529.3330696
TACAATA701.1307548E-625.1442992
GATATAC3150.023.055071
TAGCGGC2650.022.41489230
TGTAGGA6700.022.329642
TAGGACG6200.021.6449664
CTAGCGG2750.021.59980429
GTAGGAC7250.021.5446343
CGCAATA2900.021.24118636
CAATACG2900.021.24118638
GCAATAC2950.020.88116637
CCGGTCC3100.020.580469
CTGTAGG6750.020.5399721
TCTAGCG3000.020.53314828
TAGTCAG755.469496E-520.5331485
TAGGACA2800.020.4283874
ACCGGTC654.930085E-420.3075078
CGCCGGT2500.020.2398157
ATACGAA3050.020.19653740
Produced by FastQC (version 0.11.5)