##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752409
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23760082614642	32.0	32.0	32.0	32.0	32.0
2	31.409112597005088	32.0	32.0	32.0	32.0	32.0
3	31.518107837625546	32.0	32.0	32.0	32.0	32.0
4	31.622242689813653	32.0	32.0	32.0	32.0	32.0
5	31.55722884760815	32.0	32.0	32.0	32.0	32.0
6	35.182863309715856	36.0	36.0	36.0	36.0	36.0
7	35.1855373872455	36.0	36.0	36.0	36.0	36.0
8	35.12127446641388	36.0	36.0	36.0	36.0	36.0
9	35.25144303164901	36.0	36.0	36.0	36.0	36.0
10	35.13266587720243	36.0	36.0	36.0	36.0	36.0
11	35.26937343918002	36.0	36.0	36.0	36.0	36.0
12	35.17462178150448	36.0	36.0	36.0	36.0	36.0
13	35.21600618812375	36.0	36.0	36.0	36.0	36.0
14	35.17644924502498	36.0	36.0	36.0	36.0	36.0
15	35.14110809413497	36.0	36.0	36.0	36.0	36.0
16	35.13884203936954	36.0	36.0	36.0	36.0	36.0
17	35.12161204876603	36.0	36.0	36.0	36.0	36.0
18	35.138136306184535	36.0	36.0	36.0	36.0	36.0
19	35.111484578201484	36.0	36.0	36.0	36.0	36.0
20	35.10110591446939	36.0	36.0	36.0	36.0	36.0
21	35.0843822973941	36.0	36.0	36.0	36.0	36.0
22	35.05439594688527	36.0	36.0	36.0	36.0	36.0
23	35.006370205566384	36.0	36.0	36.0	36.0	36.0
24	34.97932108733415	36.0	36.0	36.0	36.0	36.0
25	34.94402911182615	36.0	36.0	36.0	36.0	36.0
26	34.895801352721726	36.0	36.0	36.0	32.0	36.0
27	34.863251237026674	36.0	36.0	36.0	32.0	36.0
28	34.83044062471342	36.0	36.0	36.0	32.0	36.0
29	34.79922356059005	36.0	36.0	36.0	32.0	36.0
30	34.78078013420892	36.0	36.0	36.0	32.0	36.0
31	34.773456989483115	36.0	36.0	36.0	32.0	36.0
32	34.73164329506957	36.0	36.0	36.0	32.0	36.0
33	34.71205554425851	36.0	36.0	36.0	32.0	36.0
34	34.68591417699682	36.0	36.0	36.0	32.0	36.0
35	34.63687568862148	36.0	36.0	36.0	32.0	36.0
36	34.596904077436605	36.0	36.0	36.0	32.0	36.0
37	34.56262086179193	36.0	36.0	36.0	32.0	36.0
38	34.49561475208298	36.0	36.0	36.0	32.0	36.0
39	34.41677731127618	36.0	36.0	36.0	32.0	36.0
40	34.39233448829028	36.0	36.0	36.0	32.0	36.0
41	34.37610262503505	36.0	36.0	36.0	32.0	36.0
42	34.30280073736492	36.0	36.0	36.0	32.0	36.0
43	34.31881463406206	36.0	36.0	36.0	32.0	36.0
44	34.28310533233919	36.0	36.0	36.0	32.0	36.0
45	34.18451400767402	36.0	36.0	36.0	32.0	36.0
46	34.215603481617045	36.0	36.0	36.0	32.0	36.0
47	34.16039680546086	36.0	36.0	36.0	32.0	36.0
48	34.15138176178116	36.0	36.0	36.0	32.0	36.0
49	34.16198769552199	36.0	36.0	36.0	32.0	36.0
50	33.63571541541901	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	5.0
21	15.0
22	64.0
23	238.0
24	652.0
25	1396.0
26	2951.0
27	5332.0
28	8945.0
29	13235.0
30	18358.0
31	25258.0
32	37691.0
33	58071.0
34	127683.0
35	452512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.957037044914365	17.478026681696612	11.453376154496487	26.111560118892534
2	15.627251195542666	20.1178131205325	37.70126186571112	26.553673818213717
3	19.576930540160497	23.642421777604113	28.258147861659793	28.522499820575593
4	12.149009777952624	15.59528287216892	36.0148164490761	36.240890900802356
5	13.965675583359582	36.96181199321114	33.70547135932718	15.367041064102105
6	34.9608321083676	34.967743307434056	16.56454927020396	13.506875313994382
7	30.091479501175556	30.127497145834248	20.609136785976776	19.17188656701342
8	27.853600900574023	34.17622596220939	19.319944338783827	18.650228798432767
9	26.795798561686528	14.217267470218989	18.36288507979038	40.624048888304095
10	15.442133201490146	27.36038510969433	32.13239076087607	25.06509092793946
11	38.030778472878445	20.477293599624673	22.196571279716217	19.29535664778066
12	24.46581579965152	23.40096941955771	29.520912163464285	22.61230261732648
13	30.57818287660036	19.323266999730198	25.031465599162157	25.067084524507283
14	22.965169209831355	20.26078901235897	24.807119532063012	31.96692224574666
15	25.15387242842656	28.40237158247708	22.336255945901765	24.107500043194594
16	25.4977013831573	25.58595125789298	25.326916610513695	23.589430748436023
17	23.482175253087085	26.407047230960824	26.05298448051525	24.057793035436845
18	24.61772785811972	24.557122522457867	27.99567788264096	22.82947173678146
19	25.431779789981245	25.571996081918215	24.93909562485297	24.05712850324757
20	25.035319885859952	24.686440486490724	26.40784466958795	23.87039495806137
21	25.844720092396557	24.815758450523585	24.08596920026209	25.25355225681777
22	24.74385606764406	25.824651220280458	25.073729846400028	24.35776286567545
23	23.42316479467949	25.142309568333182	25.60708338151192	25.827442255475415
24	24.409862189314588	25.999954811811126	25.54501607503366	24.045166923840625
25	24.83715638701823	24.928728922700287	25.386857413986274	24.847257276295206
26	23.290524169700255	26.13232962391465	26.31786701116015	24.259279195224938
27	24.725116259906514	25.62861422444442	25.60734919438763	24.038920321261443
28	23.60737311754644	25.364529132426643	25.67247334893655	25.355624401090367
29	23.701470875547738	25.340340160737046	25.88605399456944	25.072134969145772
30	24.155479267260226	25.28930408860075	26.458216209534974	24.097000434604052
31	24.533996802271105	25.03611732448708	25.252356098877073	25.177529774364743
32	23.940170837935216	25.731616713781996	25.419153678385026	24.909058769897754
33	23.434594748335012	25.088083741688365	26.530783124603772	24.94653838537285
34	23.992535974450067	25.788101949870352	26.674587890362822	23.544774185316765
35	24.75076720241252	25.5528575548671	26.237990242009335	23.45838500071105
36	23.440442631600632	25.97948722038147	26.163562636810568	24.416507511207335
37	24.770703168090762	25.428855848348437	25.541427601211574	24.259013382349227
38	24.15534636082237	25.745306076881057	25.20942731944993	24.889920242846646
39	24.69441487276202	24.762330062505896	25.681245173834977	24.862009890897106
40	25.535180998632395	25.079311916789937	25.703706361832463	23.68180072274521
41	23.985226120368043	24.864668019654204	26.992765902587557	24.157339957390196
42	25.46155083206075	26.11917188656701	25.633265949769342	22.786011331602893
43	23.966486312630497	24.96089228066118	26.066142217862893	25.006479188845425
44	23.535736547542626	25.729357304338464	26.179511409353157	24.555394738765752
45	23.782277989763546	26.01484033285088	25.789563920686753	24.413317756698817
46	23.63478325589308	24.755053109483153	26.496794292458347	25.11336934216542
47	24.854700036815082	24.614671010049054	26.46393118636274	24.066697766773125
48	24.4410952022105	26.12913986940613	24.966607257488945	24.463157670894418
49	23.822017014682174	25.42752678396989	26.2451671896535	24.505289011694437
50	23.374653944862438	26.89694036089414	24.853105159560823	24.875300534682598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	9.5
2	9.0
3	53.5
4	98.0
5	112.5
6	127.0
7	116.5
8	106.0
9	129.0
10	152.0
11	249.0
12	346.0
13	602.0
14	858.0
15	1166.5
16	1475.0
17	1689.0
18	1903.0
19	2098.5
20	2294.0
21	2754.0
22	3214.0
23	3462.5
24	3711.0
25	4508.5
26	5306.0
27	7278.5
28	9251.0
29	10953.0
30	12655.0
31	14262.5
32	15870.0
33	18802.0
34	21734.0
35	25174.0
36	28614.0
37	31415.5
38	34217.0
39	34829.0
40	35441.0
41	36574.5
42	37708.0
43	38602.5
44	39497.0
45	44495.0
46	49493.0
47	53045.5
48	56598.0
49	56648.0
50	56698.0
51	53201.5
52	49705.0
53	48807.5
54	47910.0
55	48415.5
56	48921.0
57	47108.5
58	45296.0
59	41532.0
60	37768.0
61	33541.5
62	29315.0
63	25737.0
64	22159.0
65	19093.5
66	16028.0
67	13942.0
68	11856.0
69	10989.0
70	10122.0
71	7804.0
72	5486.0
73	4954.0
74	4422.0
75	3149.5
76	1877.0
77	1680.5
78	1484.0
79	1273.0
80	1062.0
81	870.5
82	679.0
83	562.0
84	445.0
85	362.0
86	279.0
87	208.5
88	138.0
89	92.5
90	47.0
91	32.0
92	17.0
93	9.0
94	1.0
95	2.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017676556234707454
2	0.004120099573503241
3	3.9871931356482977E-4
4	2.6581287570988653E-4
5	0.0
6	9.303450649846028E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.3290643785494327E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	752409.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.09668298436755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.49496559772183	51.01265443914686
2	10.332014799114585	12.625036655425955
3	2.6735234267430186	4.900302397649946
4	1.06917037391824	2.612910535662417
5	0.5976226912380573	1.8256382055418074
6	0.3593366536815977	1.3172566568788826
7	0.25454844987873304	1.0886446162482184
8	0.17336286407647672	0.8473516758193997
9	0.12226949898863455	0.6723234736529448
>10	0.7596619871573858	8.737682745461495
>50	0.08438555663662121	3.672544784482411
>100	0.07520305715705129	8.914303071980058
>500	0.0034978166113294623	1.4381010348457968
>1k	4.372270764161828E-4	0.3352497072037661
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1391	0.18487285505622608	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1119	0.1487223039596815	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	865	0.11496406874452592	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	800	0.10632515028395462	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	771	0.10247086358616125	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	770	0.10233795714830632	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	754	0.10021145414262722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.3290643785494327E-4
7	0.0	0.0	0.0	0.0	1.3290643785494327E-4
8	0.0	0.0	0.0	0.0	1.3290643785494327E-4
9	0.0	0.0	0.0	0.0	1.3290643785494327E-4
10	0.0	0.0	0.0	0.0	1.3290643785494327E-4
11	0.0	0.0	0.0	0.0	1.3290643785494327E-4
12	0.0	0.0	0.0	0.0	5.316257514197731E-4
13	0.0	0.0	0.0	0.0	5.316257514197731E-4
14	0.0	0.0	0.0	0.0	5.316257514197731E-4
15	0.0	0.0	0.0	0.0	5.316257514197731E-4
16	0.0	0.0	0.0	0.0	5.316257514197731E-4
17	0.0	0.0	0.0	0.0	5.316257514197731E-4
18	0.0	0.0	0.0	2.6581287570988653E-4	5.316257514197731E-4
19	1.3290643785494327E-4	0.0	0.0	3.9871931356482977E-4	5.316257514197731E-4
20	1.3290643785494327E-4	0.0	0.0	0.0010632515028395461	5.316257514197731E-4
21	1.3290643785494327E-4	0.0	0.0	0.0011961579406944893	7.974386271296595E-4
22	1.3290643785494327E-4	0.0	0.0	0.0017277836921142623	7.974386271296595E-4
23	1.3290643785494327E-4	0.0	0.0	0.002923941632808752	7.974386271296595E-4
24	1.3290643785494327E-4	0.0	0.0	0.004651725324923014	7.974386271296595E-4
25	1.3290643785494327E-4	0.0	0.0	0.00611369614132739	7.974386271296595E-4
26	1.3290643785494327E-4	0.0	0.0	0.008904731336281198	9.303450649846028E-4
27	1.3290643785494327E-4	0.0	0.0	0.011297047217670176	0.0010632515028395461
28	1.3290643785494327E-4	0.0	0.0	0.016613304731867905	0.0010632515028395461
29	1.3290643785494327E-4	0.0	0.0	0.02445478456530956	0.0010632515028395461
30	1.3290643785494327E-4	0.0	0.0	0.043194592302856556	0.0010632515028395461
31	1.3290643785494327E-4	0.0	0.0	0.07615538889088248	0.0010632515028395461
32	1.3290643785494327E-4	0.0	0.0	0.1192170747558841	0.0010632515028395461
33	1.3290643785494327E-4	0.0	0.0	0.16108260268019123	0.0010632515028395461
34	1.3290643785494327E-4	0.0	0.0	0.20879601387011584	0.0010632515028395461
35	1.3290643785494327E-4	0.0	0.0	0.26594578214774145	0.0010632515028395461
36	1.3290643785494327E-4	0.0	0.0	0.3494110251206458	0.0010632515028395461
37	1.3290643785494327E-4	0.0	0.0	0.46809647412511013	0.0010632515028395461
38	1.3290643785494327E-4	0.0	0.0	0.6170845909605015	0.0011961579406944893
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	35	3.2168883E-4	31.428286	44
GTACCGT	45	4.0677776E-5	29.333069	6
TACAATA	70	1.1307548E-6	25.144299	2
GATATAC	315	0.0	23.05507	1
TAGCGGC	265	0.0	22.414892	30
TGTAGGA	670	0.0	22.32964	2
TAGGACG	620	0.0	21.644966	4
CTAGCGG	275	0.0	21.599804	29
GTAGGAC	725	0.0	21.544634	3
CGCAATA	290	0.0	21.241186	36
CAATACG	290	0.0	21.241186	38
GCAATAC	295	0.0	20.881166	37
CCGGTCC	310	0.0	20.58046	9
CTGTAGG	675	0.0	20.539972	1
TCTAGCG	300	0.0	20.533148	28
TAGTCAG	75	5.469496E-5	20.533148	5
TAGGACA	280	0.0	20.428387	4
ACCGGTC	65	4.930085E-4	20.307507	8
CGCCGGT	250	0.0	20.239815	7
ATACGAA	305	0.0	20.196537	40
>>END_MODULE