FastQCFastQC Report
Thu 2 Feb 2017
SRR4062390_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062390_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793410
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT153400.5491496056790804No Hit
GTCCTAAAGTGTGTATTTCTCATTT137570.49248051664453124No Hit
CTGTAGGACGTGGAATATGGCAAGA135480.4849986217562048No Hit
CTTTAGGACGTGAAATATGGCGAGG114390.4094995006103651No Hit
GTCCTACAGTGTGCATTTCTCATTT96270.3446325458847788No Hit
CTGTAGGACCTGGAATATGGCGAGA73350.26258229189413657No Hit
CTGAAGGACCTGGAATATGGCGAGA57450.20566261307863867No Hit
GTATCAACGCAGAGTACTTTTTTTT56190.20115199702156147No Hit
GTCCTTCAGTGTGCATTTCTCATTT47520.17011466272405412No Hit
ATTTAGAAATGTCCACTGTAGGACG46210.16542505396629925No Hit
GATATACACTGTTCTACAAATCCCG41220.14756158243866815No Hit
TTGTAGAACAGTGTATATCAATGAG40000.14319416054213308No Hit
GAATATGGCAAGAAAACTGAAAATC39160.1401870831707483No Hit
TTTCTAAATTTTCCACCTTTTTCAG38410.1375021926605833No Hit
TATCAACGCAGAGTACTTTTTTTTT35760.12801557952466697No Hit
GGAATATGGCGAGAAAACTGAAAAT32930.11788459266631106No Hit
GTGTATATCAATGAGTTACAATGAA32050.11473432113438414No Hit
CTGTAGGACATGGAATATGGCAAGA31420.11247901310584554No Hit
ACCTGGAATATGGCGAGAAAACTGA29960.10725242624605767No Hit
GTGTATATCAATGAGTTACAATGAG29080.10410215471413076No Hit
ATTCCAGGTCCTTCAGTGTGCATTT28840.10324298975087796No Hit
GACCTGGAATATGGCGAGAAAACTG28330.10141726420396575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA456.754073E-414.78084716
ACGAACG456.7568285E-414.78005115
AACCGCG456.762342E-414.77846057
GCGGTCG1058.913048E-1114.4776389
TCGGCGT1058.913048E-1114.47763813
CGGTCGG1058.913048E-1114.47763810
GCATCGC1006.5847416E-1014.25193614
CGCATCG954.9003575E-914.001413
TCGCGTA954.9003575E-914.00149
TAGGACC26150.013.9479314
TCGCGAG551.9566872E-413.821794518
CCAACGA4350.013.32484419
TATACCG500.001500964613.2989465
GGTATCA21200.013.0313531
TCCAACG6450.012.66997818
GTCGGCG1207.403287E-1012.66816112
CGCGTAA1051.9930667E-812.66793310
GGTCGGC1207.403287E-1012.6674811
GTCGCGT1051.9947038E-812.6672528
GGTCGCG1051.9947038E-812.6672527
Produced by FastQC (version 0.11.5)