##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062390_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2793410
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325805019671296	32.0	32.0	32.0	32.0	32.0
2	31.055051710991226	32.0	32.0	32.0	32.0	32.0
3	31.039161812981266	32.0	32.0	32.0	32.0	32.0
4	31.10420167465571	32.0	32.0	32.0	32.0	32.0
5	30.983115260559675	32.0	32.0	32.0	32.0	32.0
6	34.66922363706008	36.0	36.0	36.0	32.0	36.0
7	34.556712763253515	36.0	36.0	36.0	32.0	36.0
8	34.576805051889984	36.0	36.0	36.0	32.0	36.0
9	34.68117462169893	36.0	36.0	36.0	32.0	36.0
10	34.42166921432944	36.0	36.0	36.0	32.0	36.0
11	34.670489473439275	36.0	36.0	36.0	32.0	36.0
12	34.497995997723216	36.0	36.0	36.0	32.0	36.0
13	34.57344249501505	36.0	36.0	36.0	32.0	36.0
14	34.511348853193766	36.0	36.0	36.0	32.0	36.0
15	34.47156665151195	36.0	36.0	36.0	32.0	36.0
16	34.49039560966704	36.0	36.0	36.0	32.0	36.0
17	34.445788838731154	36.0	36.0	36.0	32.0	36.0
18	34.46527219419992	36.0	36.0	36.0	32.0	36.0
19	34.40616701450915	36.0	36.0	36.0	32.0	36.0
20	34.39058462595895	36.0	36.0	36.0	32.0	36.0
21	34.35989238958835	36.0	36.0	36.0	32.0	36.0
22	34.314156174711194	36.0	36.0	36.0	32.0	36.0
23	34.305837667939905	36.0	36.0	36.0	32.0	36.0
24	34.27094876870921	36.0	36.0	36.0	32.0	36.0
25	33.864689035981115	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	104.0
5	270.0
6	497.0
7	111.0
8	429.0
9	495.0
10	325.0
11	84.0
12	222.0
13	154.0
14	380.0
15	486.0
16	761.0
17	1027.0
18	1223.0
19	1660.0
20	2480.0
21	3486.0
22	5283.0
23	8097.0
24	11594.0
25	16957.0
26	24391.0
27	32513.0
28	44912.0
29	62031.0
30	81962.0
31	113210.0
32	165529.0
33	248965.0
34	597325.0
35	1366438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41161971011587	20.58746556350546	12.542205403508897	24.45870932286977
2	12.234149952887334	21.700967742859355	46.15616488574104	19.90871741851227
3	18.49356974365969	27.613782941124033	29.881512015090507	24.011135300125765
4	10.377561155154105	17.579014227824207	40.72327100320876	31.320153613812927
5	10.721518348175389	40.93083985370306	36.362086704533695	11.985555093587852
6	28.589612542253175	39.96167039982121	19.760767709442863	11.687949348482753
7	26.369452398155975	32.892936733991306	23.140004707148382	17.597606160704338
8	23.099634275288555	41.28747854184515	20.971752092606785	14.64113509025951
9	27.002596155396652	15.212825616833264	21.60685714695243	36.177721080817655
10	14.272145694514485	30.8284741136113	35.297450912293186	19.601929279581025
11	33.15205684241992	22.607542788981764	27.17928738375121	17.06111298484711
12	23.823077320149352	25.626494812248712	33.89757417423274	16.6528536933692
13	29.09721473018449	23.9814422872553	26.928432590836053	19.992910391724156
14	18.982701159683973	25.165973809795194	28.750620990444816	27.100704040076018
15	20.30122669021583	37.03814541728505	23.636195514930698	19.024432377568417
16	18.63757239520615	29.70160272951431	32.69690062503584	18.963924250243707
17	18.505542038776348	31.90765562655648	30.287732961331177	19.29906937333599
18	18.483623657409463	28.91435666775159	35.22670082974999	17.375318845088962
19	22.684855100127795	27.207552107137545	28.811568331191516	21.29602446154315
20	23.27675596822205	29.027969684424825	30.173674768860764	17.52159957849236
21	21.660021414404575	27.94178321697122	27.527367530185025	22.87082783843918
22	22.02959407903299	31.95025418238388	28.326375989149337	17.693775749433787
23	19.2759366355829	31.412442300494863	30.09286411811352	19.21875694580871
24	21.85067314020731	28.35007162286368	31.510152666392315	18.28910257053669
25	20.11140942965398	30.185574844697122	30.98035279652724	18.722662929121665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	285.0
1	285.0
2	838.0
3	1391.0
4	1391.0
5	1391.0
6	2973.5
7	4556.0
8	4556.0
9	4556.0
10	6396.0
11	8236.0
12	8236.0
13	8236.0
14	12546.5
15	16857.0
16	16857.0
17	16857.0
18	28983.0
19	41109.0
20	41109.0
21	41109.0
22	68464.5
23	95820.0
24	95820.0
25	95820.0
26	144644.5
27	193469.0
28	193469.0
29	193469.0
30	258191.0
31	322913.0
32	322913.0
33	322913.0
34	350234.0
35	377555.0
36	377555.0
37	377555.0
38	382224.0
39	386893.0
40	386893.0
41	386893.0
42	383180.0
43	379467.0
44	379467.0
45	379467.0
46	366356.0
47	353245.0
48	353245.0
49	353245.0
50	309557.5
51	265870.0
52	265870.0
53	265870.0
54	205635.0
55	145400.0
56	145400.0
57	145400.0
58	116639.5
59	87879.0
60	87879.0
61	87879.0
62	69696.0
63	51513.0
64	51513.0
65	51513.0
66	40136.0
67	28759.0
68	28759.0
69	28759.0
70	21153.0
71	13547.0
72	13547.0
73	13547.0
74	10796.0
75	8045.0
76	8045.0
77	8045.0
78	6769.0
79	5493.0
80	5493.0
81	5493.0
82	3902.5
83	2312.0
84	2312.0
85	2312.0
86	1742.5
87	1173.0
88	1173.0
89	1173.0
90	810.5
91	448.0
92	448.0
93	448.0
94	297.5
95	147.0
96	147.0
97	147.0
98	587.5
99	1028.0
100	1028.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008054671530494986
2	0.0037946452543665267
3	0.0046896087577548585
4	0.015464969338550376
5	0.036049129916482
6	0.046967684657819656
7	0.06841101019900409
8	0.08996173136059511
9	0.09826699267203884
10	0.12003250507444307
11	0.121034864198238
12	0.13467410798987617
13	0.12210882040230399
14	0.12572447295599284
15	0.1152355006962816
16	0.11348137222964047
17	0.1239345459492162
18	0.13080786565523858
19	0.13334956200486142
20	0.12260999996420147
21	0.13241879996133757
22	0.1528955649188626
23	0.14169062185644068
24	0.13596285543475536
25	0.13245459850147312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2793410.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.33845283026595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.94793938589382	47.56106509845833
2	11.783305169349129	13.512729752747079
3	2.5840262617927023	4.4449220377190795
4	0.8688564865975742	1.9927554669218246
5	0.4153681224218642	1.19082827473411
6	0.2379571616799814	0.8186457294366949
7	0.17279617429065203	0.6935505702170477
8	0.11745904337166845	0.538793585428366
9	0.09396228853220366	0.4848887023944839
>10	0.6244977895001512	7.050804906359201
>50	0.07749879096385921	3.108125464248101
>100	0.0633872718931553	7.2219303022127574
>500	0.007567499996582985	2.981598538848682
>1k	0.004878223138280493	5.444120297966475
>5k	2.5016528914258943E-4	1.0157767780535412
>10k+	2.5016528914258943E-4	1.939464494254315
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	15340	0.5491496056790804	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	13757	0.49248051664453124	No Hit
CTGTAGGACGTGGAATATGGCAAGA	13548	0.4849986217562048	No Hit
CTTTAGGACGTGAAATATGGCGAGG	11439	0.4094995006103651	No Hit
GTCCTACAGTGTGCATTTCTCATTT	9627	0.3446325458847788	No Hit
CTGTAGGACCTGGAATATGGCGAGA	7335	0.26258229189413657	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5745	0.20566261307863867	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5619	0.20115199702156147	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	4752	0.17011466272405412	No Hit
ATTTAGAAATGTCCACTGTAGGACG	4621	0.16542505396629925	No Hit
GATATACACTGTTCTACAAATCCCG	4122	0.14756158243866815	No Hit
TTGTAGAACAGTGTATATCAATGAG	4000	0.14319416054213308	No Hit
GAATATGGCAAGAAAACTGAAAATC	3916	0.1401870831707483	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3841	0.1375021926605833	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3576	0.12801557952466697	No Hit
GGAATATGGCGAGAAAACTGAAAAT	3293	0.11788459266631106	No Hit
GTGTATATCAATGAGTTACAATGAA	3205	0.11473432113438414	No Hit
CTGTAGGACATGGAATATGGCAAGA	3142	0.11247901310584554	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2996	0.10725242624605767	No Hit
GTGTATATCAATGAGTTACAATGAG	2908	0.10410215471413076	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2884	0.10324298975087796	No Hit
GACCTGGAATATGGCGAGAAAACTG	2833	0.10141726420396575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.159708027106654E-5	0.0
6	0.0	0.0	0.0	1.4319416054213308E-4	0.0
7	0.0	0.0	0.0	1.4319416054213308E-4	0.0
8	0.0	0.0	0.0	1.4319416054213308E-4	0.0
9	0.0	0.0	0.0	1.7899270067766636E-4	0.0
10	0.0	0.0	0.0	2.1479124081319963E-4	0.0
11	0.0	0.0	0.0	2.5058978094873294E-4	0.0
12	0.0	0.0	0.0	2.5058978094873294E-4	0.0
13	0.0	0.0	0.0	2.8638832108426616E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	45	6.754073E-4	14.780847	16
ACGAACG	45	6.7568285E-4	14.780051	15
AACCGCG	45	6.762342E-4	14.7784605	7
GCGGTCG	105	8.913048E-11	14.477638	9
TCGGCGT	105	8.913048E-11	14.477638	13
CGGTCGG	105	8.913048E-11	14.477638	10
GCATCGC	100	6.5847416E-10	14.251936	14
CGCATCG	95	4.9003575E-9	14.0014	13
TCGCGTA	95	4.9003575E-9	14.0014	9
TAGGACC	2615	0.0	13.947931	4
TCGCGAG	55	1.9566872E-4	13.8217945	18
CCAACGA	435	0.0	13.324844	19
TATACCG	50	0.0015009646	13.298946	5
GGTATCA	2120	0.0	13.031353	1
TCCAACG	645	0.0	12.669978	18
GTCGGCG	120	7.403287E-10	12.668161	12
CGCGTAA	105	1.9930667E-8	12.667933	10
GGTCGGC	120	7.403287E-10	12.66748	11
GTCGCGT	105	1.9947038E-8	12.667252	8
GGTCGCG	105	1.9947038E-8	12.667252	7
>>END_MODULE