FastQCFastQC Report
Thu 2 Feb 2017
SRR4062390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793410
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA77180.2762931327660458No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC60210.21554301015604582No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA51500.18436248169799635No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA51270.1835391152748791No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA30550.10936454011405414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59400.025.9303871
GGTATCA24400.025.3404251
TAGGACC34900.023.828344
TGTAGGA93250.022.4846972
CTGTAGG90800.022.2945861
GTAGGAC91350.022.1327023
CTCGGAA1700.021.99984737
AGGACCT60350.021.5081065
GTCCTAC81200.021.163741
GGACCTG60100.020.9386656
GACGTTA755.476219E-520.5331927
TCCTACA87150.020.3222372
TAGGACG93700.020.1218874
AGGACGT92500.020.0261675
CCTACAG87500.019.9636483
GGACGTG90850.019.7844776
GACGTGG46650.019.665467
GATATAC23700.019.589871
TCGGAAA1800.019.5554238
TAGGACA24150.019.4950374
Produced by FastQC (version 0.11.5)