##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062390_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2793410
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33630723739086	32.0	32.0	32.0	32.0	32.0
2	31.528840377889388	32.0	32.0	32.0	32.0	32.0
3	31.59526743299408	32.0	32.0	32.0	32.0	32.0
4	31.673953340182788	32.0	32.0	32.0	32.0	32.0
5	31.652407988802217	32.0	32.0	32.0	32.0	32.0
6	35.27031513454882	36.0	36.0	36.0	36.0	36.0
7	35.28140946012222	36.0	36.0	36.0	36.0	36.0
8	35.23494796682191	36.0	36.0	36.0	36.0	36.0
9	35.34393017852732	36.0	36.0	36.0	36.0	36.0
10	35.2668516257907	36.0	36.0	36.0	36.0	36.0
11	35.352912390232724	36.0	36.0	36.0	36.0	36.0
12	35.2949545537533	36.0	36.0	36.0	36.0	36.0
13	35.31863528805295	36.0	36.0	36.0	36.0	36.0
14	35.29812881030711	36.0	36.0	36.0	36.0	36.0
15	35.2629417092371	36.0	36.0	36.0	36.0	36.0
16	35.291342481053626	36.0	36.0	36.0	36.0	36.0
17	35.28275512724591	36.0	36.0	36.0	36.0	36.0
18	35.288679785638344	36.0	36.0	36.0	36.0	36.0
19	35.257321338435816	36.0	36.0	36.0	36.0	36.0
20	35.250885118904854	36.0	36.0	36.0	36.0	36.0
21	35.22540550796339	36.0	36.0	36.0	36.0	36.0
22	35.20905631468349	36.0	36.0	36.0	36.0	36.0
23	35.19201406166656	36.0	36.0	36.0	36.0	36.0
24	35.17919030861921	36.0	36.0	36.0	36.0	36.0
25	35.14634192617625	36.0	36.0	36.0	36.0	36.0
26	35.119171550184184	36.0	36.0	36.0	36.0	36.0
27	35.08560182715749	36.0	36.0	36.0	36.0	36.0
28	35.05083106310925	36.0	36.0	36.0	36.0	36.0
29	35.02354290992013	36.0	36.0	36.0	36.0	36.0
30	34.990421026630536	36.0	36.0	36.0	36.0	36.0
31	34.97901847562657	36.0	36.0	36.0	36.0	36.0
32	34.957830035691146	36.0	36.0	36.0	36.0	36.0
33	34.9715541220229	36.0	36.0	36.0	36.0	36.0
34	34.94942095861331	36.0	36.0	36.0	36.0	36.0
35	34.93904152988641	36.0	36.0	36.0	36.0	36.0
36	34.88582664198954	36.0	36.0	36.0	36.0	36.0
37	34.84692508439506	36.0	36.0	36.0	36.0	36.0
38	34.813530058244226	36.0	36.0	36.0	36.0	36.0
39	34.70932551970531	36.0	36.0	36.0	36.0	36.0
40	34.758770463340504	36.0	36.0	36.0	36.0	36.0
41	34.72688398767098	36.0	36.0	36.0	36.0	36.0
42	34.72981266623947	36.0	36.0	36.0	36.0	36.0
43	34.70959579868333	36.0	36.0	36.0	36.0	36.0
44	34.655072116159104	36.0	36.0	36.0	32.0	36.0
45	34.63163839178638	36.0	36.0	36.0	32.0	36.0
46	34.54609455826392	36.0	36.0	36.0	32.0	36.0
47	34.54397134684847	36.0	36.0	36.0	32.0	36.0
48	34.49724458636577	36.0	36.0	36.0	32.0	36.0
49	34.446115321417196	36.0	36.0	36.0	32.0	36.0
50	34.018046760053124	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	23.0
21	56.0
22	201.0
23	635.0
24	1609.0
25	3660.0
26	7413.0
27	14133.0
28	24124.0
29	37200.0
30	53769.0
31	77382.0
32	113582.0
33	186640.0
34	412267.0
35	1860710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88176101574699	21.27323549514694	12.915886585613427	24.929116903492652
2	11.916433636907476	22.238252776219472	45.6255502050101	20.21976338186295
3	18.079716759922533	27.942750564726015	30.242107260353908	23.73542541499755
4	10.272571060356555	18.12121429082838	40.84134030214076	30.764874346674304
5	10.576682469341224	41.14163017302515	36.18732523593931	12.094362121694317
6	28.788051272247962	40.09344161706765	19.45788683476277	11.660620275921618
7	26.298189774572933	33.04396169698028	23.178668071879198	17.479180456567583
8	23.09890889590461	41.501298234522764	20.760976999787715	14.63881586978491
9	26.79463451480449	15.153092456889608	21.58397800537694	36.46829502292896
10	14.207975198771393	31.076211512094538	35.16182730068268	19.55398598845139
11	33.13545093631082	22.6058115350056	27.24966975846725	17.00906777021633
12	23.884070007625088	25.72218184942418	33.733823534676254	16.659924608274473
13	29.079082555013407	23.879129809086386	27.01615588116317	20.02563175473704
14	19.005086972553258	25.193473210162487	28.665716812068403	27.135723005215844
15	20.351541664130938	37.060546070931224	23.56027221209919	19.027640052838645
16	18.76086933174865	29.86657884091487	32.380101739451064	18.992450087885416
17	18.60099305150336	32.12732824755407	30.03629971969743	19.235378981245145
18	18.62590883543769	29.007915057223965	35.028585134298226	17.337590973040122
19	22.91729463272488	27.22242706942411	28.81553370253561	21.044744595315404
20	23.46411733329515	29.11727959733802	29.985251001464157	17.43335206790267
21	21.730680422852355	27.946703133446217	27.537991200718835	22.784625242982592
22	22.19205200811911	31.781371155684273	28.32638245012368	17.700194386072937
23	19.307334046917568	31.441177628776302	29.932161766443166	19.319326557862972
24	22.03088698042894	28.34714560340229	31.23963184781325	18.382335568355522
25	20.21994623059272	30.217690922564177	30.71894208154192	18.843420765301193
26	17.511070698536912	31.702399576145286	31.779402235976818	19.007127489340984
27	18.96599496672526	30.952670750086813	30.46459345387895	19.61674082930898
28	18.044254155315546	31.77553599364218	28.897977740467745	21.28223211057453
29	20.036013331376346	29.296057506774876	28.48271467489556	22.18521448695322
30	18.54657927049735	29.851794043838893	30.52190691663594	21.079719769027818
31	21.793148875388862	28.31890055523536	29.61409173733895	20.27385883203683
32	19.376353632298876	30.875703888795414	29.9403596321342	19.80758284677151
33	18.446737142059348	31.040806755900498	31.757278738173056	18.7551773638671
34	19.602135024933684	30.90402053404262	31.584335990778296	17.909508450245397
35	18.83765720033937	31.444506893008906	30.939532685857067	18.778303220794655
36	17.92440064294178	30.50858985970552	32.30914903290244	19.25786046445026
37	18.681396572647767	29.930729824837744	29.169652861556305	22.218220740958184
38	19.399909071708056	31.851214107488694	29.010671544814404	19.738205275988847
39	21.41053407842028	28.386273407770428	30.0878496174926	20.115342896316687
40	22.696274445928093	28.21683891730895	30.666855205644715	18.42003143111824
41	21.720263047672915	27.306124056260984	32.091350714717855	18.882262181348246
42	20.062468452536507	30.95327932526912	30.939174700455712	18.045077521738666
43	18.077761588882403	30.98642877343462	31.74482084620589	19.190988791477086
44	17.724000415263067	30.889378931127187	30.796302726774805	20.590317926834945
45	18.048693174292353	31.207592154391943	30.288607830572666	20.455106840743035
46	18.6968391298371	28.280258379728274	30.982835303686397	22.04006718674823
47	21.24690655754313	28.39365807155343	30.323056881394738	20.036378489508696
48	18.32663303990463	29.47250851110292	30.187405357609514	22.013453091382935
49	20.630949269888774	28.604107524495152	29.891458826309066	20.873484379307012
50	18.376090558948267	31.78237184914336	29.04335100470501	20.798186587203368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	29.5
2	37.0
3	124.5
4	212.0
5	264.0
6	316.0
7	334.5
8	353.0
9	545.5
10	738.0
11	1180.0
12	1622.0
13	2712.0
14	3802.0
15	5403.0
16	7004.0
17	8210.5
18	9417.0
19	11058.5
20	12700.0
21	15734.0
22	18768.0
23	23618.0
24	28468.0
25	36768.0
26	45068.0
27	61630.0
28	78192.0
29	98276.0
30	118360.0
31	143255.0
32	168150.0
33	203876.5
34	239603.0
35	275246.0
36	310889.0
37	311300.0
38	311711.0
39	288615.0
40	265519.0
41	241160.0
42	216801.0
43	198028.5
44	179256.0
45	166815.5
46	154375.0
47	144414.0
48	134453.0
49	124731.5
50	115010.0
51	101668.0
52	88326.0
53	78670.0
54	69014.0
55	63673.5
56	58333.0
57	51903.0
58	45473.0
59	39283.5
60	33094.0
61	27966.0
62	22838.0
63	19388.0
64	15938.0
65	13500.5
66	11063.0
67	9784.5
68	8506.0
69	8102.5
70	7699.0
71	5816.5
72	3934.0
73	3527.5
74	3121.0
75	2225.5
76	1330.0
77	1314.5
78	1299.0
79	1113.0
80	927.0
81	759.5
82	592.0
83	543.5
84	495.0
85	418.0
86	341.0
87	252.0
88	163.0
89	107.5
90	52.0
91	33.5
92	15.0
93	10.0
94	5.0
95	2.5
96	0.0
97	1.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015429170798414842
2	0.004761205838025926
3	7.159708027106655E-4
4	3.5798540135533277E-4
5	3.579854013553328E-5
6	2.863883210842662E-4
7	3.579854013553328E-5
8	3.579854013553328E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.159708027106655E-5
47	3.579854013553328E-5
48	0.0
49	0.0
50	1.0739562040659982E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2793410.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.63501403473121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.2693850955277	57.83830428729713
2	11.1651931120989	15.326463718987846
3	2.377235699254089	4.894848167465054
4	0.8383600218943854	2.3016340747551483
5	0.3380211672023314	1.160004377748412
6	0.19601884905404895	0.8072253873537905
7	0.12772222006058853	0.6136351456483865
8	0.09459256863675101	0.5193889820771745
9	0.06883531003509748	0.425206122330959
>10	0.440340231465581	5.764140687444529
>50	0.04666315882043008	2.2235922539600383
>100	0.03369638572545754	4.59424421377429
>500	0.0023617081348393815	1.1567881779147
>1k	0.0013645424778494322	1.509436750507818
>5k	2.0992961197683572E-4	0.8650876527347213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	7718	0.2762931327660458	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	6021	0.21554301015604582	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	5150	0.18436248169799635	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	5127	0.1835391152748791	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3055	0.10936454011405414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.579854013553327E-5	0.0
6	0.0	0.0	0.0	3.579854013553327E-5	0.0
7	0.0	0.0	0.0	3.579854013553327E-5	0.0
8	0.0	0.0	0.0	3.579854013553327E-5	0.0
9	0.0	0.0	0.0	7.159708027106654E-5	0.0
10	0.0	0.0	0.0	7.159708027106654E-5	0.0
11	0.0	0.0	0.0	1.4319416054213308E-4	0.0
12	0.0	0.0	0.0	1.4319416054213308E-4	0.0
13	0.0	0.0	0.0	1.7899270067766636E-4	0.0
14	0.0	0.0	0.0	2.1479124081319963E-4	0.0
15	0.0	0.0	0.0	2.1479124081319963E-4	0.0
16	0.0	0.0	0.0	3.2218686121979944E-4	0.0
17	0.0	0.0	0.0	3.2218686121979944E-4	0.0
18	0.0	0.0	0.0	4.6538102176193254E-4	0.0
19	0.0	0.0	0.0	5.36978102032999E-4	0.0
20	0.0	0.0	0.0	0.001181351824472598	0.0
21	0.0	0.0	0.0	0.0015035386856923974	0.0
22	0.0	0.0	0.0	0.002398502189080729	0.0
23	0.0	0.0	0.0	0.003293465692469061	0.0
24	0.0	0.0	0.0	0.004295824816263992	0.0
25	0.0	0.0	0.0	0.004797004378161458	0.0
26	0.0	0.0	0.0	0.005835162042091923	0.0
27	0.0	0.0	0.0	0.007732484669275187	0.0
28	0.0	0.0	0.0	0.013388654010689444	0.0
29	0.0	0.0	0.0	0.02283946860647023	0.0
30	0.0	0.0	0.0	0.04460498100887446	0.0
31	0.0	0.0	0.0	0.10227642916721856	0.0
32	0.0	0.0	0.0	0.17841992403549783	0.0
33	0.0	0.0	0.0	0.2576778918955685	0.0
34	0.0	0.0	0.0	0.353975964860153	0.0
35	0.0	0.0	0.0	0.46215915314973455	0.0
36	0.0	0.0	0.0	0.6063556728156626	0.0
37	0.0	0.0	0.0	0.8293805778600348	0.0
38	0.0	0.0	0.0	1.1128692171933228	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5940	0.0	25.930387	1
GGTATCA	2440	0.0	25.340425	1
TAGGACC	3490	0.0	23.82834	4
TGTAGGA	9325	0.0	22.484697	2
CTGTAGG	9080	0.0	22.294586	1
GTAGGAC	9135	0.0	22.132702	3
CTCGGAA	170	0.0	21.999847	37
AGGACCT	6035	0.0	21.508106	5
GTCCTAC	8120	0.0	21.16374	1
GGACCTG	6010	0.0	20.938665	6
GACGTTA	75	5.476219E-5	20.533192	7
TCCTACA	8715	0.0	20.322237	2
TAGGACG	9370	0.0	20.121887	4
AGGACGT	9250	0.0	20.026167	5
CCTACAG	8750	0.0	19.963648	3
GGACGTG	9085	0.0	19.784477	6
GACGTGG	4665	0.0	19.66546	7
GATATAC	2370	0.0	19.58987	1
TCGGAAA	180	0.0	19.55542	38
TAGGACA	2415	0.0	19.495037	4
>>END_MODULE