FastQCFastQC Report
Thu 2 Feb 2017
SRR4062389_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062389_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341303
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG148744.358004471100459No Hit
TCCATGTACTCTGCGTTGATACCAC129123.7831486977846662No Hit
GAGTACATGGAAGCAGTGGTATCAA64231.8819055209007833No Hit
CATGTACTCTGCGTTGATACCACTG55821.6354969045100687No Hit
GCTTCCATGTACTCTGCGTTGATAC44431.3017758414077814No Hit
CCCATGTACTCTGCGTTGATACCAC39361.153227484083058No Hit
GCGTTGATACCACTGCTTCCATGTA35241.032513631582494No Hit
CATGGAAGCAGTGGTATCAACGCAG35161.0301696732815124No Hit
GTACATGGGAAGCAGTGGTATCAAC33410.9788955854475349No Hit
GTATCAACGCAGAGTACATGGAAGC29030.8505638684687798No Hit
ACTCTGCGTTGATACCACTGCTTCC24080.7055314485955295No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23240.6809198864352204No Hit
CAGTGGTATCAACGCAGAGTACATG22080.6469324910709838No Hit
ACGCAGAGTACATGGAAGCAGTGGT22060.6463465014957384No Hit
TATCAACGCAGAGTACATGGAAGCA20120.5895055126969292No Hit
GTCCTAAAGTGTGTATTTCTCATTT18580.544384315403029No Hit
GTCCTACAGTGGACATTTCTAAATT17980.5268046281456653No Hit
GTATCAACGCAGAGTACTTTTTTTT16460.4822694204270106No Hit
CTGTAGGACGTGGAATATGGCAAGA15940.4670336914706287No Hit
CTTTAGGACGTGAAATATGGCGAGG14920.43714822313311047No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14510.4251354368405786No Hit
ATACCACTGCTTCCATGTACTCTGC14310.4192755410881241No Hit
GTCCTACAGTGTGCATTTCTCATTT13420.39319900498970123No Hit
GGTATCAACGCAGAGTACATGGAAG13330.39056205190109666No Hit
GTATCAACGCAGAGTACATGGGAAG13260.3885110883877376No Hit
CTGTAGGACCTGGAATATGGCGAGA12450.3647785105902966No Hit
TATCAACGCAGAGTACTTTTTTTTT12290.36009059398833293No Hit
AAGCAGTGGTATCAACGCAGAGTAC12070.3536447086606329No Hit
GAGTACATGGGAAGCAGTGGTATCA11770.3448548650319511No Hit
AAAAAGTACTCTGCGTTGATACCAC11420.3346000474651556No Hit
GATATACACTGTTCTACAAATCCCG10880.31877832893352825No Hit
GCGTTGATACCACTGCTTCCCATGT10860.31819233935828284No Hit
TTGTAGAACAGTGTATATCAATGAG10710.3137974175439419No Hit
GCTTCCCATGTACTCTGCGTTGATA10470.3067655426409964No Hit
GTACTCTGCGTTGATACCACTGCTT10270.3009056468885419No Hit
CCACTGCTTCCATGTACTCTGCGTT9890.28977184495887814No Hit
CTGAAGGACCTGGAATATGGCGAGA9810.28742788665789637No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9540.27951702739208273No Hit
GTGGTATCAACGCAGAGTACATGGA8730.25578444959464175No Hit
GTCCTTCAGTGTGCATTTCTCATTT8580.25138952778030077No Hit
TATCAACGCAGAGTACATGGGAAGC8560.2508035382050553No Hit
GTGTATATCAATGAGTTACAATGAA8520.24963155905456444No Hit
GTGTATATCAATGAGTTACAATGAG8240.241427705001128No Hit
ATTTAGAAATGTCCACTGTAGGACG8160.2390837467001462No Hit
GAAGCAGTGGTATCAACGCAGAGTA8030.23527481446105075No Hit
ACATGGAAGCAGTGGTATCAACGCA8020.234981819673428No Hit
GTTCTACAGTGTGGTTTTTATCATT7980.23380984052293713No Hit
CTGCTTCCATGTACTCTGCGTTGAT7480.21916010114180068No Hit
GTTGATACCACTGCTTCCATGTACT7260.21271421581410066No Hit
CCCCATGTACTCTGCGTTGATACCA7070.20714731484926885No Hit
TGGAAGCAGTGGTATCAACGCAGAG7010.20538934612353243No Hit
GATACCACTGCTTCCATGTACTCTG7000.20509635133590973No Hit
ACGCAGAGTACATGGGAAGCAGTGG6980.20451036176066428No Hit
GGTATCAACGCAGAGTACATGGGAA6770.198357471220587No Hit
GTACATGGGGTGGTATCAACGCAAA6440.18868864322903695No Hit
TTTCTAAATTTTCCACCTTTTTCAG6070.17784783608699603No Hit
GGTATCAACGCAGAGTACTTTTTTT5910.17315991948503237No Hit
GCAGAGTACATGGAAGCAGTGGTAT5810.1702299716088051No Hit
CTTCCATGTACTCTGCGTTGATACC5550.16261210713061414No Hit
GCAGTGGTATCAACGCAGAGTACAT5500.1611471331925005No Hit
GATATACACTGTTCTACAATGCCGG5430.15909616967914142No Hit
CCATGTACTCTGCGTTGATACCACT5330.15616622180291412No Hit
AAAGTACTCTGCGTTGATACCACTG5010.14679038859898683No Hit
GAATATGGCAAGAAAACTGAAAATC4850.14210247199702317No Hit
GGAATATGGCGAGAAAACTGAAAAT4700.13770755018268224No Hit
GTTCTACAAATCCCGTTTCCAACGA4560.13360562315596405No Hit
CTGTAGAACATATTAGATGAGTGAG4510.1321406492178504No Hit
CTGCTTCCCATGTACTCTGCGTTGA4330.1268667430406413No Hit
ATGTTCTACAGTGTGGTTTTTATCA4300.12598775867777312No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4280.12540176910252765No Hit
CTGTAGGACATGGAATATGGCAAGA4150.12159283686343221No Hit
ATTCCAGGTCCTTCAGTGTGCATTT4060.11895588377482764No Hit
GTAGAACAGTGTATATCAATGAGTT3930.11514695153573218No Hit
CCATATTCCAGGTCCTTCAGTGTGC3910.11456096196048673No Hit
GTACATGGGTGGTATCAACGCAAAA3890.11397497238524126No Hit
TGATACCACTGCTTCCATGTACTCT3860.11309598802237308No Hit
ACCTGGAATATGGCGAGAAAACTGA3820.11192400887188217No Hit
GTGGTATCAACGCAGAGTACATGGG3790.11104502450901399No Hit
CACTGCTTCCATGTACTCTGCGTTG3760.1101660401461458No Hit
ATCAACGCAGAGTACATGGAAGCAG3710.10870106620803216No Hit
GACCTGGAATATGGCGAGAAAACTG3660.10723609226991851No Hit
ACAGTGGACATTTCTAAATTTTCCA3610.10577111833180487No Hit
GTCCACTGTAGGACGTGGAATATGG3600.10547812354418215No Hit
ATACACACTTTAGGACGTGAAATAT3600.10547812354418215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG307.7026454E-419.0011735
TAGGACC4050.014.5419764
GGAGTGG851.6994818E-814.5303098
ACAGGTC400.005265770514.2508798
TTGTAGA1950.014.1249351
TAGAAAT2800.013.90953354
AAAAAGT2000.013.77181151
GCCGGTT655.4250355E-513.15465811
CCGGTTT655.4325086E-513.1527312
TTAGAAA2900.013.1004153
ACTGTTC3850.013.0787318
TTTAGAA3300.012.9515462
ATTTAGA2900.012.7729051
AATGTCC3400.012.5743068
AAATGTC3100.012.5652927
AAAAGTA2200.012.5198292
GAAATGT3050.012.4597856
GAGTGGT1001.4284706E-712.3507619
ATGTCCA3350.012.1947839
TGTCCAC3350.012.19478310
Produced by FastQC (version 0.11.5)