##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062389_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341303
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.988874987913967	32.0	32.0	32.0	32.0	32.0
2	30.983337386427895	32.0	32.0	32.0	32.0	32.0
3	30.94151238049475	32.0	32.0	32.0	32.0	32.0
4	30.903727772682924	32.0	32.0	32.0	32.0	32.0
5	30.958749263850596	32.0	32.0	32.0	32.0	32.0
6	34.49659686554176	36.0	36.0	36.0	32.0	36.0
7	34.34629932933493	36.0	36.0	36.0	32.0	36.0
8	34.36839992616532	36.0	36.0	36.0	32.0	36.0
9	34.336727189623296	36.0	36.0	36.0	32.0	36.0
10	34.27254668139454	36.0	36.0	36.0	32.0	36.0
11	34.45013375212055	36.0	36.0	36.0	32.0	36.0
12	34.334957501106054	36.0	36.0	36.0	32.0	36.0
13	34.34564302101066	36.0	36.0	36.0	32.0	36.0
14	34.28229461797875	36.0	36.0	36.0	32.0	36.0
15	34.324427268438896	36.0	36.0	36.0	32.0	36.0
16	34.29512778967662	36.0	36.0	36.0	32.0	36.0
17	34.26074778129697	36.0	36.0	36.0	32.0	36.0
18	34.319543045329226	36.0	36.0	36.0	32.0	36.0
19	34.26582245101859	36.0	36.0	36.0	32.0	36.0
20	34.21615690456867	36.0	36.0	36.0	32.0	36.0
21	34.094857062492856	36.0	36.0	36.0	32.0	36.0
22	34.06539057670164	36.0	36.0	36.0	32.0	36.0
23	33.93099093767122	36.0	36.0	36.0	32.0	36.0
24	33.98230311482759	36.0	36.0	36.0	32.0	36.0
25	33.37481651201425	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	8.0
5	28.0
6	60.0
7	13.0
8	43.0
9	58.0
10	40.0
11	15.0
12	24.0
13	13.0
14	89.0
15	121.0
16	148.0
17	215.0
18	307.0
19	442.0
20	703.0
21	954.0
22	1267.0
23	1719.0
24	2175.0
25	2737.0
26	3551.0
27	4363.0
28	5926.0
29	7993.0
30	10400.0
31	14369.0
32	20704.0
33	30558.0
34	72095.0
35	160163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28235542324011	17.457629105229845	14.137989638763216	23.122025832766827
2	8.655764796197921	23.47115477589243	43.80067216155318	24.07240826635647
3	21.63200365675709	29.40554256547295	28.328147067269093	20.634306710500873
4	11.298848957967039	19.423248235928455	35.73211899570997	33.54578381039454
5	9.201297806800296	41.005477870166565	36.67505883801155	13.118165485021585
6	29.97822142624743	33.38511729066335	21.50099220016356	15.135669082925657
7	23.28210480641246	32.668887025559535	24.56359979594589	19.485408372082112
8	25.540818331373476	38.34744333760495	23.861400365384107	12.250337965637472
9	26.17244777064834	18.064434773428427	19.198465671151567	36.56465178477167
10	15.0062478367232	30.80388832505177	36.26326565332426	17.92659818490077
11	32.88990448924037	21.5846689977237	23.90966371764485	21.615762795391078
12	21.71415495106438	24.833947473419858	34.74663787640529	18.705259699110474
13	34.49818422889862	22.820341329766325	25.012173586543934	17.66930085479111
14	23.005415109504895	22.785701294815457	25.415520184923352	28.793363410756296
15	24.227705231541773	31.64179367146528	29.306430607863156	14.824070489129799
16	21.85467952121029	25.383128558146083	35.57427487879205	17.18791704185157
17	20.180237794199183	27.6602235957792	34.9395540405359	17.219984569485717
18	23.03700248482555	22.101159096309726	40.37533480603986	14.486503612824869
19	21.66934613218175	33.053945268494175	26.34687147953436	18.92983711978971
20	20.06723300401286	25.353178607467203	38.785112524347035	15.794475864172904
21	19.17547004309523	30.496988614527993	23.773076776394728	26.55446456598205
22	18.395779355107525	34.39221129045983	26.903090073092507	20.30891928134014
23	17.030221771058567	31.91813335602191	26.945814397662293	24.105830475257225
24	19.161764490149192	33.39152375783003	28.43692695879238	19.009784793228395
25	24.006594831506163	27.600601983730012	26.56737259917917	21.82543058558466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	116.0
2	343.5
3	571.0
4	571.0
5	571.0
6	1704.5
7	2838.0
8	2838.0
9	2838.0
10	2514.5
11	2191.0
12	2191.0
13	2191.0
14	1600.5
15	1010.0
16	1010.0
17	1010.0
18	1832.0
19	2654.0
20	2654.0
21	2654.0
22	5201.5
23	7749.0
24	7749.0
25	7749.0
26	13304.5
27	18860.0
28	18860.0
29	18860.0
30	26611.0
31	34362.0
32	34362.0
33	34362.0
34	33408.5
35	32455.0
36	32455.0
37	32455.0
38	31903.0
39	31351.0
40	31351.0
41	31351.0
42	37031.0
43	42711.0
44	42711.0
45	42711.0
46	71219.5
47	99728.0
48	99728.0
49	99728.0
50	70674.0
51	41620.0
52	41620.0
53	41620.0
54	26130.0
55	10640.0
56	10640.0
57	10640.0
58	7996.5
59	5353.0
60	5353.0
61	5353.0
62	4265.5
63	3178.0
64	3178.0
65	3178.0
66	2524.5
67	1871.0
68	1871.0
69	1871.0
70	1438.5
71	1006.0
72	1006.0
73	1006.0
74	775.0
75	544.0
76	544.0
77	544.0
78	375.0
79	206.0
80	206.0
81	206.0
82	136.5
83	67.0
84	67.0
85	67.0
86	47.0
87	27.0
88	27.0
89	27.0
90	21.0
91	15.0
92	15.0
93	15.0
94	15.5
95	16.0
96	16.0
97	16.0
98	90.0
99	164.0
100	164.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009082838416304573
2	0.004687916601963651
3	0.004980911389586379
4	0.013770755018268225
5	0.032815416213745556
6	0.041605259842427404
7	0.06357986891413202
8	0.08555447798583664
9	0.08994939980017755
10	0.11280299323475035
11	0.11690492026146856
12	0.13096867006735952
13	0.117490909836714
14	0.11866288898720492
15	0.10870106620803216
16	0.10547812354418215
17	0.12305781080154583
18	0.12715973782826404
19	0.1286247117663777
20	0.11690492026146856
21	0.12598775867777312
22	0.14708338338660953
23	0.13360562315596405
24	0.13712156060743677
25	0.12598775867777312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	341303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.378303149986962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34071198480218	21.380415642405723
2	12.946022961922854	7.347723284002777
3	4.277484100107376	3.641632215362889
4	1.9864541174527133	2.254887885544516
5	1.1243495498471958	1.595356618605755
6	0.7557611299248369	1.2868331072390222
7	0.5306847278433964	1.054195245866576
8	0.37994548608243167	0.8625766547613118
9	0.2581151400016519	0.6592382721511384
>10	1.9451556950524491	10.761405554595184
>50	0.2240439415214339	4.429788194068028
>100	0.16622615016106385	9.663261090585198
>500	0.028908895680185016	5.997017313062001
>1k	0.03200627736020484	17.407113327453906
>5k	0.0020649211200132155	3.5174024254108516
>10k+	0.0020649211200132155	8.141153168885126
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14874	4.358004471100459	No Hit
TCCATGTACTCTGCGTTGATACCAC	12912	3.7831486977846662	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6423	1.8819055209007833	No Hit
CATGTACTCTGCGTTGATACCACTG	5582	1.6354969045100687	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4443	1.3017758414077814	No Hit
CCCATGTACTCTGCGTTGATACCAC	3936	1.153227484083058	No Hit
GCGTTGATACCACTGCTTCCATGTA	3524	1.032513631582494	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3516	1.0301696732815124	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3341	0.9788955854475349	No Hit
GTATCAACGCAGAGTACATGGAAGC	2903	0.8505638684687798	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2408	0.7055314485955295	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2324	0.6809198864352204	No Hit
CAGTGGTATCAACGCAGAGTACATG	2208	0.6469324910709838	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2206	0.6463465014957384	No Hit
TATCAACGCAGAGTACATGGAAGCA	2012	0.5895055126969292	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1858	0.544384315403029	No Hit
GTCCTACAGTGGACATTTCTAAATT	1798	0.5268046281456653	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1646	0.4822694204270106	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1594	0.4670336914706287	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1492	0.43714822313311047	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1451	0.4251354368405786	No Hit
ATACCACTGCTTCCATGTACTCTGC	1431	0.4192755410881241	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1342	0.39319900498970123	No Hit
GGTATCAACGCAGAGTACATGGAAG	1333	0.39056205190109666	No Hit
GTATCAACGCAGAGTACATGGGAAG	1326	0.3885110883877376	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1245	0.3647785105902966	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1229	0.36009059398833293	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1207	0.3536447086606329	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1177	0.3448548650319511	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1142	0.3346000474651556	No Hit
GATATACACTGTTCTACAAATCCCG	1088	0.31877832893352825	No Hit
GCGTTGATACCACTGCTTCCCATGT	1086	0.31819233935828284	No Hit
TTGTAGAACAGTGTATATCAATGAG	1071	0.3137974175439419	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1047	0.3067655426409964	No Hit
GTACTCTGCGTTGATACCACTGCTT	1027	0.3009056468885419	No Hit
CCACTGCTTCCATGTACTCTGCGTT	989	0.28977184495887814	No Hit
CTGAAGGACCTGGAATATGGCGAGA	981	0.28742788665789637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	954	0.27951702739208273	No Hit
GTGGTATCAACGCAGAGTACATGGA	873	0.25578444959464175	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	858	0.25138952778030077	No Hit
TATCAACGCAGAGTACATGGGAAGC	856	0.2508035382050553	No Hit
GTGTATATCAATGAGTTACAATGAA	852	0.24963155905456444	No Hit
GTGTATATCAATGAGTTACAATGAG	824	0.241427705001128	No Hit
ATTTAGAAATGTCCACTGTAGGACG	816	0.2390837467001462	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	803	0.23527481446105075	No Hit
ACATGGAAGCAGTGGTATCAACGCA	802	0.234981819673428	No Hit
GTTCTACAGTGTGGTTTTTATCATT	798	0.23380984052293713	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	748	0.21916010114180068	No Hit
GTTGATACCACTGCTTCCATGTACT	726	0.21271421581410066	No Hit
CCCCATGTACTCTGCGTTGATACCA	707	0.20714731484926885	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	701	0.20538934612353243	No Hit
GATACCACTGCTTCCATGTACTCTG	700	0.20509635133590973	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	698	0.20451036176066428	No Hit
GGTATCAACGCAGAGTACATGGGAA	677	0.198357471220587	No Hit
GTACATGGGGTGGTATCAACGCAAA	644	0.18868864322903695	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	607	0.17784783608699603	No Hit
GGTATCAACGCAGAGTACTTTTTTT	591	0.17315991948503237	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	581	0.1702299716088051	No Hit
CTTCCATGTACTCTGCGTTGATACC	555	0.16261210713061414	No Hit
GCAGTGGTATCAACGCAGAGTACAT	550	0.1611471331925005	No Hit
GATATACACTGTTCTACAATGCCGG	543	0.15909616967914142	No Hit
CCATGTACTCTGCGTTGATACCACT	533	0.15616622180291412	No Hit
AAAGTACTCTGCGTTGATACCACTG	501	0.14679038859898683	No Hit
GAATATGGCAAGAAAACTGAAAATC	485	0.14210247199702317	No Hit
GGAATATGGCGAGAAAACTGAAAAT	470	0.13770755018268224	No Hit
GTTCTACAAATCCCGTTTCCAACGA	456	0.13360562315596405	No Hit
CTGTAGAACATATTAGATGAGTGAG	451	0.1321406492178504	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	433	0.1268667430406413	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	430	0.12598775867777312	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	428	0.12540176910252765	No Hit
CTGTAGGACATGGAATATGGCAAGA	415	0.12159283686343221	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	406	0.11895588377482764	No Hit
GTAGAACAGTGTATATCAATGAGTT	393	0.11514695153573218	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	391	0.11456096196048673	No Hit
GTACATGGGTGGTATCAACGCAAAA	389	0.11397497238524126	No Hit
TGATACCACTGCTTCCATGTACTCT	386	0.11309598802237308	No Hit
ACCTGGAATATGGCGAGAAAACTGA	382	0.11192400887188217	No Hit
GTGGTATCAACGCAGAGTACATGGG	379	0.11104502450901399	No Hit
CACTGCTTCCATGTACTCTGCGTTG	376	0.1101660401461458	No Hit
ATCAACGCAGAGTACATGGAAGCAG	371	0.10870106620803216	No Hit
GACCTGGAATATGGCGAGAAAACTG	366	0.10723609226991851	No Hit
ACAGTGGACATTTCTAAATTTTCCA	361	0.10577111833180487	No Hit
GTCCACTGTAGGACGTGGAATATGG	360	0.10547812354418215	No Hit
ATACACACTTTAGGACGTGAAATAT	360	0.10547812354418215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.929947876227282E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	30	7.7026454E-4	19.001173	5
TAGGACC	405	0.0	14.541976	4
GGAGTGG	85	1.6994818E-8	14.530309	8
ACAGGTC	40	0.0052657705	14.250879	8
TTGTAGA	195	0.0	14.124935	1
TAGAAAT	280	0.0	13.9095335	4
AAAAAGT	200	0.0	13.7718115	1
GCCGGTT	65	5.4250355E-5	13.154658	11
CCGGTTT	65	5.4325086E-5	13.15273	12
TTAGAAA	290	0.0	13.100415	3
ACTGTTC	385	0.0	13.078731	8
TTTAGAA	330	0.0	12.951546	2
ATTTAGA	290	0.0	12.772905	1
AATGTCC	340	0.0	12.574306	8
AAATGTC	310	0.0	12.565292	7
AAAAGTA	220	0.0	12.519829	2
GAAATGT	305	0.0	12.459785	6
GAGTGGT	100	1.4284706E-7	12.350761	9
ATGTCCA	335	0.0	12.194783	9
TGTCCAC	335	0.0	12.194783	10
>>END_MODULE