##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341303
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129365988579064	32.0	32.0	32.0	32.0	32.0
2	31.534448862154743	32.0	32.0	32.0	32.0	32.0
3	31.591949089225704	32.0	32.0	32.0	32.0	32.0
4	31.6803924958175	32.0	32.0	32.0	32.0	32.0
5	31.650961169400798	32.0	32.0	32.0	32.0	32.0
6	35.28251436406946	36.0	36.0	36.0	36.0	36.0
7	35.28270774062929	36.0	36.0	36.0	36.0	36.0
8	35.258500511275905	36.0	36.0	36.0	36.0	36.0
9	35.290668409009	36.0	36.0	36.0	36.0	36.0
10	35.25481756679549	36.0	36.0	36.0	36.0	36.0
11	35.29930003545237	36.0	36.0	36.0	36.0	36.0
12	35.232259898096416	36.0	36.0	36.0	36.0	36.0
13	35.24738135908562	36.0	36.0	36.0	36.0	36.0
14	35.22389782685766	36.0	36.0	36.0	36.0	36.0
15	35.210200320536295	36.0	36.0	36.0	36.0	36.0
16	35.20983114710389	36.0	36.0	36.0	36.0	36.0
17	35.22267017869752	36.0	36.0	36.0	36.0	36.0
18	35.22396814560669	36.0	36.0	36.0	36.0	36.0
19	35.15216391300399	36.0	36.0	36.0	36.0	36.0
20	35.11054400342218	36.0	36.0	36.0	36.0	36.0
21	35.003715173907054	36.0	36.0	36.0	36.0	36.0
22	34.98818352021517	36.0	36.0	36.0	36.0	36.0
23	34.86236276856635	36.0	36.0	36.0	32.0	36.0
24	34.884565913572395	36.0	36.0	36.0	32.0	36.0
25	34.86235104877484	36.0	36.0	36.0	32.0	36.0
26	34.79357638227616	36.0	36.0	36.0	32.0	36.0
27	34.765410793341985	36.0	36.0	36.0	32.0	36.0
28	34.638576279727985	36.0	36.0	36.0	32.0	36.0
29	34.61963709665605	36.0	36.0	36.0	32.0	36.0
30	34.54154812585884	36.0	36.0	36.0	32.0	36.0
31	34.44310187721761	36.0	36.0	36.0	32.0	36.0
32	34.38519145744397	36.0	36.0	36.0	32.0	36.0
33	34.33008499778789	36.0	36.0	36.0	32.0	36.0
34	34.23208410122384	36.0	36.0	36.0	32.0	36.0
35	34.06101030462668	36.0	36.0	36.0	32.0	36.0
36	33.96343132055681	36.0	36.0	36.0	32.0	36.0
37	33.77099820394195	36.0	36.0	36.0	32.0	36.0
38	33.53341752050231	36.0	36.0	36.0	21.0	36.0
39	33.09733872834402	36.0	36.0	36.0	14.0	36.0
40	32.83622470356252	36.0	36.0	36.0	14.0	36.0
41	32.959194615927785	36.0	36.0	36.0	14.0	36.0
42	32.53964951963505	36.0	36.0	36.0	14.0	36.0
43	32.52950311013967	36.0	36.0	36.0	14.0	36.0
44	32.63179345039452	36.0	36.0	36.0	14.0	36.0
45	32.033275418030314	36.0	36.0	36.0	14.0	36.0
46	32.24797027860874	36.0	36.0	36.0	14.0	36.0
47	32.084209631910646	36.0	36.0	36.0	14.0	36.0
48	32.03101642821774	36.0	36.0	36.0	14.0	36.0
49	32.23650246262119	36.0	36.0	36.0	14.0	36.0
50	31.320908401039546	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	14.0
22	38.0
23	121.0
24	364.0
25	884.0
26	1835.0
27	3398.0
28	5882.0
29	9291.0
30	13770.0
31	20575.0
32	31086.0
33	40052.0
34	58181.0
35	155810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.234139194139196	18.09992673992674	14.378901098901098	23.287032967032967
2	8.465920078760922	23.947363794588703	43.37300680367785	24.21370932297252
3	21.439140231057046	29.713068523092517	28.544305466435787	20.30348577941465
4	11.321904583317464	19.604867229411987	35.879555702704046	33.1936724845665
5	9.25306838791338	41.609654764241746	36.0143333050105	13.122943542834372
6	30.110460005859945	33.478757691180775	21.406680339876942	15.004101963082334
7	23.824285165966895	32.66833283035895	24.044910241046814	19.46247176262734
8	26.049580577961517	38.38231717857739	23.364283349399216	12.203818894061873
9	26.584882054948245	17.604005824736376	18.971119503784028	36.839992616531354
10	15.31249359073902	31.180505298810736	35.62142729480843	17.88557381564182
11	33.038385247126456	21.66081165416067	23.728182875626644	21.57262022308623
12	21.96757719680167	25.246481865087624	34.24142184510537	18.544519093005334
13	34.871653633281866	22.807593252916032	24.802594761839185	17.518158351962917
14	23.42522626522474	22.844510596156496	25.00593314444936	28.724329994169405
15	24.423459506655377	32.01261049565928	28.908037726008857	14.655892271676487
16	22.134291230959	25.576101001163188	35.111323369557255	17.178284398320553
17	20.44488328552635	28.288939739762032	34.15674635148241	17.109430623229212
18	23.428742202676215	22.37601193074775	39.58095885474198	14.61428701183406
19	22.12755235084368	33.179022745185364	25.90484115287589	18.788583751095068
20	20.714731484926883	25.76391066002936	38.01460872011087	15.50674913493289
21	19.46217876783972	30.33169939906769	23.43782504109252	26.76829679200007
22	18.8715012759923	34.30822465668336	26.40205330747166	20.41822075985268
23	17.23278142881838	32.15940088425827	26.208090758065417	24.399726928857934
24	19.622446916669354	33.69850250364046	27.591026155644695	19.088024424045496
25	24.562046041200926	27.6607589150989	25.712343577407758	22.064851466292414
26	14.721523104103978	29.036955432562856	31.27690058393861	24.964620879394555
27	22.228342557785897	32.17844554545375	29.895430160297447	15.69778173646291
28	19.708880379018055	27.232986525169718	27.49111493306534	25.567018162746884
29	18.024746339762615	30.549980515846624	30.462375074347424	20.962898070043334
30	23.512538711936315	27.021444288506107	30.339317263545883	19.126699736011695
31	18.70888916886169	27.399114569751802	29.57430787306294	24.31768838832357
32	17.70948394828056	32.08527320298972	30.367737757945285	19.837505090784436
33	16.784499403755607	30.569025177042104	31.710239874832624	20.936235544369666
34	15.434379422390077	33.85613369938149	34.077051769249024	16.63243510897941
35	20.457189066606503	31.95547651207285	30.38443846083978	17.202895960480863
36	18.202887170637233	32.55787379542518	31.273970636062387	17.965268397875203
37	20.915725909236073	31.20365188703293	29.19487962309151	18.68574258063949
38	20.68103708435027	33.22765987993074	24.16826104663598	21.923041989083014
39	20.04523839520895	28.765349264436583	31.19134610595278	19.99806623440169
40	25.677184202893034	29.050433192793502	25.509298189585206	19.76308441472826
41	18.31949909611108	27.220680744089563	32.96015563883118	21.499664520968174
42	25.33613827010017	32.15734992074491	28.453016820830758	14.053494988324159
43	20.766591562336107	28.02084950908723	28.021728493450098	23.190830435126557
44	19.257375411291434	29.984500575734756	33.557572010793926	17.20055200217988
45	22.5274902359487	29.658397377110663	29.129248790664015	18.68486359627662
46	18.988992185829016	26.603633721356097	34.01083494724629	20.3965391455686
47	25.928866725460953	25.304787827824544	32.022279323650835	16.744066123063668
48	17.348807364716983	36.46349431443615	25.844777221413228	20.342921099433642
49	18.695411408631042	26.600996768267493	37.1693187578193	17.534273065282168
50	16.995798442435145	37.62943082665792	22.827876777751083	22.546893953155855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	20.0
2	21.0
3	105.5
4	190.0
5	193.0
6	196.0
7	175.0
8	154.0
9	206.0
10	258.0
11	346.0
12	434.0
13	734.0
14	1034.0
15	1375.0
16	1716.0
17	1955.0
18	2194.0
19	2411.5
20	2629.0
21	2937.5
22	3246.0
23	3600.5
24	3955.0
25	4038.0
26	4121.0
27	6027.0
28	7933.0
29	9964.5
30	11996.0
31	14181.5
32	16367.0
33	20447.0
34	24527.0
35	28289.5
36	32052.0
37	31082.5
38	30113.0
39	25660.5
40	21208.0
41	18328.5
42	15449.0
43	14640.0
44	13831.0
45	23521.0
46	33211.0
47	41401.5
48	49592.0
49	41524.0
50	33456.0
51	22745.0
52	12034.0
53	8332.5
54	4631.0
55	4179.5
56	3728.0
57	3318.0
58	2908.0
59	2612.0
60	2316.0
61	2048.0
62	1780.0
63	1481.0
64	1182.0
65	1057.0
66	932.0
67	795.0
68	658.0
69	568.5
70	479.0
71	382.5
72	286.0
73	244.0
74	202.0
75	169.5
76	137.0
77	97.5
78	58.0
79	50.5
80	43.0
81	27.0
82	11.0
83	10.0
84	9.0
85	7.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015528723744004594
2	0.004980911389586379
3	5.859895752454564E-4
4	0.0
5	0.0
6	8.789843628681846E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	2.929947876227282E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	341303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.45442797337037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1673148104968	34.401703792778704
2	11.634959925907795	10.111810561468218
3	3.648048264396787	4.7557155164582685
4	1.627698926555265	2.829229030653121
5	0.8889254748511828	1.9313874010307388
6	0.5610638446645052	1.46284250558616
7	0.3766736336521203	1.1457696098102632
8	0.3000252236882229	1.0429939578363372
9	0.22495209195339022	0.8797648029522827
>10	1.3332702136300878	11.061868809986034
>50	0.12495015438094428	3.821290812928834
>100	0.08510118622702151	7.197057858267349
>500	0.014858937277733917	4.168494117974519
>1k	0.010806499838351939	9.529160531042503
>5k	6.754062398969962E-4	2.4404019285332765
>10k+	6.754062398969962E-4	3.220508762693402
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	10973	3.215031804584197	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	8315	2.4362516590829846	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	3966	1.16201732771174	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3964	1.1614313381364947	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	3237	0.9484241275347711	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	2570	0.7529966041904115	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	2321	0.6800409020723521	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	2261	0.6624612148149884	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2057	0.6026902781399519	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1871	0.5481932476421245	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1545	0.452676946877115	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1443	0.4227914785395968	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1424	0.4172245775747649	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	1404	0.4113646818223104	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	1242	0.36389952622742844	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1136	0.33284207873941923	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1026	0.3006126521009191	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	1001	0.2932877824103509	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	890	0.2607653609842281	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	885	0.25930038704611447	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	830	0.24318567372686442	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	710	0.208026299212137	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	673	0.19718549207009606	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	672	0.19689249728247335	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	671	0.19659950249485061	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	665	0.19484153376911423	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	656	0.1922045806805097	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	651	0.19073960674239604	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	637	0.18663767971567785	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	624	0.1828287474765824	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	616	0.18048478917560057	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	591	0.17315991948503237	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	583	0.17081596118405054	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	577	0.16905799245831415	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	573	0.16788601330782327	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	569	0.16671403415733232	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	561	0.1643700758563505	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	547	0.1602681488296323	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	521	0.15265028435144137	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	501	0.14679038859898683	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA	500	0.1464973938113641	No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	499	0.14620439902374135	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	494	0.14473942508562773	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	456	0.13360562315596405	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	454	0.13301963358071858	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	443	0.12979669091686857	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	439	0.1286247117663777	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	420	0.12305781080154583	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	417	0.12217882643867765	No Hit
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA	398	0.11661192547384581	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG	393	0.11514695153573218	No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT	384	0.11250999844712763	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	380	0.1113380192966367	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	354	0.10372015481844579	No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG	345	0.10108320172984121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.929947876227282E-4	0.0
14	0.0	0.0	0.0	2.929947876227282E-4	0.0
15	0.0	0.0	0.0	5.859895752454564E-4	0.0
16	0.0	0.0	0.0	5.859895752454564E-4	0.0
17	0.0	0.0	0.0	5.859895752454564E-4	0.0
18	0.0	0.0	0.0	8.789843628681846E-4	0.0
19	0.0	0.0	0.0	0.0014649739381136409	0.0
20	0.0	0.0	0.0	0.0017579687257363692	0.0
21	0.0	0.0	0.0	0.00322294266385001	0.0
22	0.0	0.0	0.0	0.0038089322390954666	0.0
23	0.0	0.0	0.0	0.004394921814340923	0.0
24	0.0	0.0	0.0	0.010254817566795487	0.0
25	0.0	0.0	0.0	0.012598775867777313	0.0
26	0.0	0.0	0.0	0.016407708106872778	0.0
27	0.0	0.0	0.0	0.02109562470883643	0.0
28	0.0	0.0	0.0	0.02900648397465009	0.0
29	0.0	0.0	0.0	0.04248424420529559	0.0
30	0.0	0.0	0.0	0.0632868741265093	0.0
31	0.0	0.0	0.0	0.09756726427836848	0.0
32	0.0	0.0	0.0	0.15206429477619593	0.0
33	0.0	0.0	0.0	0.2068543200616461	0.0
34	0.0	0.0	0.0	0.2762940847282327	0.0
35	0.0	0.0	0.0	0.355695672173992	0.0
36	0.0	0.0	0.0	0.46000181656768324	0.0
37	0.0	0.0	0.0	0.6305247829641111	2.929947876227282E-4
38	0.0	0.0	0.0	0.8001687649976706	2.929947876227282E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGTAA	20	7.848951E-4	43.99971	43
TAGAGTG	40	4.113408E-7	38.49975	5
TCGTATA	35	7.2755047E-6	37.714035	44
GCGGTGA	25	0.0023505117	35.199768	42
TATCCCT	25	0.0023505117	35.199768	42
TGGTATA	380	0.0	31.841894	44
GTCCTAT	35	3.2076694E-4	31.437574	1
GTAAGAC	35	3.2131883E-4	31.428364	3
CTAGAGT	50	2.3550238E-6	30.799797	4
TAATACA	30	0.005739499	29.333138	4
CTAATGT	30	0.005739499	29.333138	4
GGTGATG	40	7.021998E-4	27.499819	23
TAGGACC	415	0.0	27.035963	4
CGAAGCA	50	8.324596E-5	26.399826	36
GTTGTAT	60	9.726744E-6	25.666494	43
GCACTGG	80	1.3041608E-7	24.749838	40
GTCCTAG	45	0.001393833	24.451447	1
CCTAGAG	45	0.0013962153	24.444283	3
TCCTAGA	45	0.0013962153	24.444283	2
AAAGGGT	45	0.0013962153	24.444283	34
>>END_MODULE