FastQCFastQC Report
Thu 2 Feb 2017
SRR4062388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966068
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22220.23000451313986178No Hit
TATCAACGCAGAGTACTTTTTTTTT14610.1512315903228344No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13200.13663634443952186No Hit
GTATTAGAGGCACTGCCTGCCCAGT11580.11986733853103508No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11270.11665845468434935No Hit
CCATAGGGTCTTCTCGTCTTATTAT11100.11489874418777973No Hit
GCGCAAGACGGACCAGAGCGAAAGC10730.11106878604818708No Hit
GTACATGGAAGCAGTGGTATCAACG10620.10993014984452441No Hit
GATTAAGAGGGACGGCCGGGGGCAT10170.10527209264772253No Hit
CTCTAATACTTGTAATGCTAGAGGT10010.10361589453330407No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9850.10195969641888564No Hit
TCCATGTACTCTGCGTTGATACCAC9780.10123510974382756No Hit
GAATAGGACCGCGGTTCTATTTTGT9750.1009245725973741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA651.908229E-716.079039
TAGACTG551.1287941E-515.5434615
AGCCCCG1400.014.25334516
AGACGAT801.2846249E-714.2518667
CCGTCGT1403.6379788E-1212.8945469
CGTCGTA1403.6379788E-1212.89454610
GACGATC905.3892654E-712.6683258
CGATCAG905.3892654E-712.66832510
GTCGTAG1501.8189894E-1212.66701211
TTCGAAG853.962916E-612.2874452
AGACCGT550.003063986312.0924936
TAAAGGC550.003071134212.0887334
CGAGGTT550.003071134212.0887334
CTTAAAC550.003077101712.0855993
CGACCAT1501.4551915E-1112.0349110
TCCAACG951.034814E-612.0034418
AATCGTA951.0387103E-611.99970513
GAAGACG951.0400126E-611.9984615
ATCCTAG1201.007902E-811.8679391
TATATCT1459.640644E-1111.7909794
Produced by FastQC (version 0.11.5)