##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062388_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	966068
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21306160642936	32.0	32.0	32.0	32.0	32.0
2	31.003108476835997	32.0	32.0	32.0	32.0	32.0
3	30.98945312338262	32.0	32.0	32.0	32.0	32.0
4	30.97421817097761	32.0	32.0	32.0	32.0	32.0
5	30.92864891498321	32.0	32.0	32.0	32.0	32.0
6	34.457688278671895	36.0	36.0	36.0	32.0	36.0
7	34.38401437579963	36.0	36.0	36.0	32.0	36.0
8	34.385311385947986	36.0	36.0	36.0	32.0	36.0
9	34.4830043019746	36.0	36.0	36.0	32.0	36.0
10	34.26873056555025	36.0	36.0	36.0	32.0	36.0
11	34.47747467052009	36.0	36.0	36.0	32.0	36.0
12	34.3289157699044	36.0	36.0	36.0	32.0	36.0
13	34.415114671016944	36.0	36.0	36.0	32.0	36.0
14	34.33427357080454	36.0	36.0	36.0	32.0	36.0
15	34.28561239995528	36.0	36.0	36.0	32.0	36.0
16	34.29379298351669	36.0	36.0	36.0	32.0	36.0
17	34.24799185978627	36.0	36.0	36.0	32.0	36.0
18	34.26271132052816	36.0	36.0	36.0	32.0	36.0
19	34.23511595457049	36.0	36.0	36.0	32.0	36.0
20	34.2065434317253	36.0	36.0	36.0	32.0	36.0
21	34.17140511848027	36.0	36.0	36.0	32.0	36.0
22	34.137363001362225	36.0	36.0	36.0	32.0	36.0
23	34.11993048108415	36.0	36.0	36.0	32.0	36.0
24	34.100933888711765	36.0	36.0	36.0	32.0	36.0
25	33.576134392195996	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	42.0
5	95.0
6	140.0
7	34.0
8	151.0
9	208.0
10	130.0
11	42.0
12	67.0
13	68.0
14	169.0
15	270.0
16	427.0
17	566.0
18	727.0
19	963.0
20	1298.0
21	1691.0
22	2225.0
23	3155.0
24	4303.0
25	5921.0
26	8619.0
27	11401.0
28	15875.0
29	22094.0
30	30326.0
31	44062.0
32	67131.0
33	102831.0
34	242980.0
35	398051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65060632599779	19.261331991693428	11.465120468083256	25.622941214225527
2	15.940395225821144	20.81850460130638	38.34270157241493	24.89839860045754
3	18.570990836552756	24.996376856602197	29.521475923694528	26.91115638315052
4	11.632019214841808	16.253209375517642	38.23525757826735	33.879513831373195
5	13.381247883762518	37.68875285141968	34.74905953629677	14.18093972852103
6	31.827318698821582	37.77926444956955	17.636495629219453	12.756921222389414
7	28.25875511571803	31.983816152151373	21.872743827214844	17.88468490491575
8	26.58945883025776	34.08893820575702	20.54306574788098	18.778537216104237
9	26.78964617917651	14.613203079910434	20.17556857214797	38.421582168765084
10	15.358960274441117	26.92642532154591	32.915004093815746	24.799610310197227
11	36.03859156739637	21.991887023590646	23.772223108924067	18.197298300088924
12	23.505125786489483	25.336519026048737	29.83715651011164	21.32119867735014
13	27.972800565463018	20.444763196814502	26.861462596631892	24.720973641090588
14	22.334666565784605	21.49762184359204	26.105306361892282	30.06240522873107
15	24.532940070617325	28.555661148759636	23.338332025796984	23.57306675482605
16	24.676685386562923	26.74793015430183	25.2185916654835	23.35679279365175
17	22.311712890076414	26.841050823812786	26.904169901217088	23.943066384893708
18	23.148722073539076	25.789569698766336	28.42367993896966	22.638028288724936
19	24.35826075059004	25.915537630563424	26.561708029242475	23.164493589604056
20	24.055610370873996	25.93837836941471	26.517221222648768	23.488790037062525
21	25.61855493791907	25.100306906484214	25.526306788323666	23.754831367273052
22	24.087504976114648	26.16524018046709	26.20432341427813	23.542931429140125
23	23.434607291310343	25.59453099895305	26.77530034932778	24.195561360408828
24	23.485041658633467	26.656964631104973	26.32993554780222	23.52805816245934
25	23.529850769474756	26.00707541547076	26.862116467599346	23.60095734745514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	116.0
2	359.0
3	602.0
4	602.0
5	602.0
6	1788.5
7	2975.0
8	2975.0
9	2975.0
10	3174.0
11	3373.0
12	3373.0
13	3373.0
14	3850.0
15	4327.0
16	4327.0
17	4327.0
18	7329.0
19	10331.0
20	10331.0
21	10331.0
22	16251.0
23	22171.0
24	22171.0
25	22171.0
26	33344.5
27	44518.0
28	44518.0
29	44518.0
30	53878.0
31	63238.0
32	63238.0
33	63238.0
34	72741.0
35	82244.0
36	82244.0
37	82244.0
38	85647.5
39	89051.0
40	89051.0
41	89051.0
42	94755.0
43	100459.0
44	100459.0
45	100459.0
46	112167.5
47	123876.0
48	123876.0
49	123876.0
50	119343.5
51	114811.0
52	114811.0
53	114811.0
54	102353.0
55	89895.0
56	89895.0
57	89895.0
58	80942.0
59	71989.0
60	71989.0
61	71989.0
62	64290.5
63	56592.0
64	56592.0
65	56592.0
66	47569.5
67	38547.0
68	38547.0
69	38547.0
70	29557.0
71	20567.0
72	20567.0
73	20567.0
74	16483.5
75	12400.0
76	12400.0
77	12400.0
78	10340.0
79	8280.0
80	8280.0
81	8280.0
82	5796.5
83	3313.0
84	3313.0
85	3313.0
86	2346.0
87	1379.0
88	1379.0
89	1379.0
90	941.0
91	503.0
92	503.0
93	503.0
94	295.5
95	88.0
96	88.0
97	88.0
98	255.5
99	423.0
100	423.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00890206486499915
2	0.003933470521743811
3	0.005796693400464564
4	0.015319832558370633
5	0.03322747467052009
6	0.04668408435016997
7	0.06842168460191209
8	0.09119440867516572
9	0.1009245725973741
10	0.1240078338170812
11	0.12359378428847657
12	0.1384995673182426
13	0.12369729667062773
14	0.12887291577818538
15	0.1189357270916747
16	0.11810762803446549
17	0.12680266813516233
18	0.1347731215608011
19	0.13529068347155687
20	0.12545700716719735
21	0.1323923367713246
22	0.15112807794068328
23	0.14056981496126567
24	0.13684336920382417
25	0.13653283205737068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	966068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.627987426492595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.39104450642566	38.60626714034581
2	12.708398039009452	12.359676401036296
3	3.5641333495461924	5.199498951242256
4	1.3555284302084116	2.6366647784171153
5	0.668222369859094	1.6247154499804555
6	0.4095340961253153	1.1948891326261104
7	0.2772994099975361	0.9439158555913802
8	0.20021541964267403	0.7788858327179113
9	0.15387433370929565	0.6734339248388
>10	0.9535045552881474	9.301984133773345
>50	0.14824587404607306	5.20013423728479
>100	0.15293566472174014	15.308403649699557
>500	0.014930957492873797	4.869700605526651
>1k	0.0021329939275533997	1.3018299069195296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2222	0.23000451313986178	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1461	0.1512315903228344	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1320	0.13663634443952186	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1158	0.11986733853103508	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1127	0.11665845468434935	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1110	0.11489874418777973	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1073	0.11106878604818708	No Hit
GTACATGGAAGCAGTGGTATCAACG	1062	0.10993014984452441	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1017	0.10527209264772253	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1001	0.10361589453330407	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	985	0.10195969641888564	No Hit
TCCATGTACTCTGCGTTGATACCAC	978	0.10123510974382756	No Hit
GAATAGGACCGCGGTTCTATTTTGT	975	0.1009245725973741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.0351238215115292E-4	0.0
4	0.0	0.0	0.0	1.0351238215115292E-4	0.0
5	0.0	0.0	0.0	2.0702476430230584E-4	0.0
6	0.0	0.0	0.0	2.0702476430230584E-4	0.0
7	0.0	0.0	0.0	3.105371464534588E-4	0.0
8	0.0	0.0	0.0	3.105371464534588E-4	0.0
9	0.0	0.0	0.0	3.105371464534588E-4	0.0
10	0.0	0.0	0.0	3.105371464534588E-4	0.0
11	0.0	0.0	0.0	3.105371464534588E-4	0.0
12	0.0	0.0	0.0	3.105371464534588E-4	0.0
13	0.0	0.0	0.0	3.105371464534588E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCA	65	1.908229E-7	16.07903	9
TAGACTG	55	1.1287941E-5	15.543461	5
AGCCCCG	140	0.0	14.253345	16
AGACGAT	80	1.2846249E-7	14.251866	7
CCGTCGT	140	3.6379788E-12	12.894546	9
CGTCGTA	140	3.6379788E-12	12.894546	10
GACGATC	90	5.3892654E-7	12.668325	8
CGATCAG	90	5.3892654E-7	12.668325	10
GTCGTAG	150	1.8189894E-12	12.667012	11
TTCGAAG	85	3.962916E-6	12.287445	2
AGACCGT	55	0.0030639863	12.092493	6
TAAAGGC	55	0.0030711342	12.088733	4
CGAGGTT	55	0.0030711342	12.088733	4
CTTAAAC	55	0.0030771017	12.085599	3
CGACCAT	150	1.4551915E-11	12.03491	10
TCCAACG	95	1.034814E-6	12.00344	18
AATCGTA	95	1.0387103E-6	11.999705	13
GAAGACG	95	1.0400126E-6	11.998461	5
ATCCTAG	120	1.007902E-8	11.867939	1
TATATCT	145	9.640644E-11	11.790979	4
>>END_MODULE