FastQCFastQC Report
Thu 2 Feb 2017
SRR4062388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966068
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT12300.12732023004591808No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10670.11044771175528016No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG10650.11024068699097786No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10590.10961961269807094No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10030.10382291929760638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG1004.622052E-821.99979414
ACTGCGT500.002580207321.9997948
ACGGTAA500.002580207321.99979442
ATAACGC3200.019.9383453
GTACTAA808.9797E-519.251811
TCTAGAC1152.019442E-719.1312453
TAACGCC3250.018.9546474
ACCGACT708.1222237E-418.85696441
CGAATGC3500.018.85696443
TTAACGG4100.018.78031235
AATAACG3450.018.4944932
CTAGACA1557.203198E-1018.4523944
AACGCCG3350.018.388845
CGCAATA3350.018.38788836
ATACGAA3350.018.38788840
AACAACG600.00741145418.333167
CCGGATC600.00741145418.3331627
ATCGTTT3400.018.11747729
GTATTAG5250.018.0207441
CATCGTT3300.017.99983228
Produced by FastQC (version 0.11.5)