##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	966068
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21528712264561	32.0	32.0	32.0	32.0	32.0
2	31.283485220502076	32.0	32.0	32.0	32.0	32.0
3	31.410332398961565	32.0	32.0	32.0	32.0	32.0
4	31.537237544355055	32.0	32.0	32.0	32.0	32.0
5	31.45218556043674	32.0	32.0	32.0	32.0	32.0
6	35.01841588790851	36.0	36.0	36.0	36.0	36.0
7	35.03598090403574	36.0	36.0	36.0	36.0	36.0
8	34.96936965099765	36.0	36.0	36.0	36.0	36.0
9	35.123033782301036	36.0	36.0	36.0	36.0	36.0
10	34.97913500912979	36.0	36.0	36.0	36.0	36.0
11	35.139605079559615	36.0	36.0	36.0	36.0	36.0
12	35.03325645813752	36.0	36.0	36.0	36.0	36.0
13	35.09300691048663	36.0	36.0	36.0	36.0	36.0
14	35.04706604504031	36.0	36.0	36.0	36.0	36.0
15	34.99810986390192	36.0	36.0	36.0	36.0	36.0
16	35.025751810431565	36.0	36.0	36.0	36.0	36.0
17	35.00137050393968	36.0	36.0	36.0	36.0	36.0
18	35.01151885788578	36.0	36.0	36.0	36.0	36.0
19	34.99342282323811	36.0	36.0	36.0	36.0	36.0
20	34.981356384850756	36.0	36.0	36.0	36.0	36.0
21	34.96492896980337	36.0	36.0	36.0	32.0	36.0
22	34.957014413064094	36.0	36.0	36.0	32.0	36.0
23	34.91736710045256	36.0	36.0	36.0	32.0	36.0
24	34.89965820211414	36.0	36.0	36.0	32.0	36.0
25	34.87526861463168	36.0	36.0	36.0	32.0	36.0
26	34.834155566688885	36.0	36.0	36.0	32.0	36.0
27	34.816918684813075	36.0	36.0	36.0	32.0	36.0
28	34.79739728466319	36.0	36.0	36.0	32.0	36.0
29	34.776086155425915	36.0	36.0	36.0	32.0	36.0
30	34.75387757383538	36.0	36.0	36.0	32.0	36.0
31	34.75964735401649	36.0	36.0	36.0	32.0	36.0
32	34.71944003941751	36.0	36.0	36.0	32.0	36.0
33	34.70144337665671	36.0	36.0	36.0	32.0	36.0
34	34.690272320374966	36.0	36.0	36.0	32.0	36.0
35	34.66084478525321	36.0	36.0	36.0	32.0	36.0
36	34.63549874335968	36.0	36.0	36.0	32.0	36.0
37	34.62626026325269	36.0	36.0	36.0	32.0	36.0
38	34.57820049934373	36.0	36.0	36.0	32.0	36.0
39	34.57058819876033	36.0	36.0	36.0	32.0	36.0
40	34.5769862991011	36.0	36.0	36.0	32.0	36.0
41	34.53499546615766	36.0	36.0	36.0	32.0	36.0
42	34.49812021514013	36.0	36.0	36.0	32.0	36.0
43	34.50475535883602	36.0	36.0	36.0	32.0	36.0
44	34.46171284008993	36.0	36.0	36.0	32.0	36.0
45	34.41390668151724	36.0	36.0	36.0	32.0	36.0
46	34.40522820339769	36.0	36.0	36.0	32.0	36.0
47	34.39189373832898	36.0	36.0	36.0	32.0	36.0
48	34.35112538661875	36.0	36.0	36.0	32.0	36.0
49	34.332170199199226	36.0	36.0	36.0	32.0	36.0
50	33.78204122277107	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	18.0
22	47.0
23	215.0
24	570.0
25	1319.0
26	2829.0
27	5340.0
28	9284.0
29	15212.0
30	22814.0
31	34093.0
32	52225.0
33	86844.0
34	189211.0
35	546043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29913949462909	19.83230911936924	11.971397927830186	25.89715345817149
2	15.51213159826134	21.51193905785632	38.025591311251915	24.950338032630423
3	17.816643428016597	25.524629346119248	29.907883854365608	26.750843371498544
4	11.62385553767086	16.87194961001589	38.454244797192736	33.04995005512051
5	13.32639110290373	37.97486305311841	34.473660239237816	14.22508560474004
6	31.796138363074196	38.29651566609769	17.37809075353987	12.529255217288243
7	28.504618722491585	32.0386349615141	21.703751702778685	17.752994613215634
8	26.65216113151455	34.301933197249056	20.30353970942004	18.74236596181635
9	27.12086519789497	14.448051275893622	19.80409246554073	38.626991060670676
10	15.44456497886277	26.994373066906263	32.802142292261	24.758919661969962
11	36.51782276195878	21.872166348538613	23.603928501927403	18.0060823875752
12	23.70640576025704	25.255365046766894	29.589014437907064	21.449214755069
13	28.25298012148213	20.40394672010666	26.53260433012997	24.81046882828124
14	22.38362102874746	21.572808539357478	25.785037906234344	30.25853252566072
15	24.592989313381665	28.63338812588762	22.994344083439263	23.779278477291456
16	24.80881263016682	26.82150738871384	24.942240090759658	23.427439890359686
17	22.485787749930648	26.96104207985359	26.41584236306347	24.137327807152293
18	23.488305171064564	25.94982961861898	27.89203244492106	22.669832765395398
19	24.712960164294852	26.10157877085257	26.107893026163794	23.077568038688785
20	24.30574245291222	26.135841369344604	25.953349039612117	23.605067138131062
21	26.018458327985194	25.229279926464805	24.93633988497704	23.81592186057296
22	24.35905132972006	26.276514696688018	25.647262925591157	23.71717104800076
23	23.647403702430886	25.736697623769757	26.26771614420517	24.34818252959419
24	23.71427270130053	26.9792602591122	25.828306081973523	23.47816095761375
25	23.849252847625632	26.12828496544757	26.306429775129704	23.7160324117971
26	22.86671331624689	26.814986108638315	27.26505794623153	23.053242628883268
27	23.985164605390096	26.521839042386254	26.007900065005774	23.48509628721788
28	22.971467846983856	26.265231847033544	26.777721651063903	23.9855786549187
29	23.22507318325418	26.05820708273124	26.448552275823232	24.268167458191346
30	23.35570580952894	26.397417159040565	26.819437141070818	23.427439890359686
31	23.936099736250448	26.030051714786122	25.82954823055934	24.204300318404087
32	23.01038850267269	26.59657498229938	26.051064728362803	24.34197178666512
33	23.14888806999093	26.22755333993052	26.52328821573637	24.100270374342177
34	23.53219442109665	26.153541986692446	27.300666205691527	23.013597386519375
35	24.50707403619621	26.207471937793198	26.199087434838958	23.086366591171636
36	23.217620291739298	26.371849600649227	26.240906437228023	24.16962367038345
37	24.431095947697262	26.221446109383606	25.523048067009775	23.824409875909357
38	23.362020064840156	26.37837088072475	26.294939900710922	23.96466915372417
39	23.69729667062774	26.235937842884766	25.607928220373722	24.458837266113772
40	24.42840462576133	26.252292799264648	26.556101640878282	22.763200934095735
41	23.34462998463876	26.13190789882286	27.162166638373282	23.361295478165097
42	24.334208358003785	27.184628825300084	25.98202196946799	22.499140847228148
43	23.002832098775656	26.19401532811355	26.605684071928682	24.19746850118211
44	23.01753085704112	26.378888442635507	26.412736991598933	24.190843708724437
45	23.150958317633954	26.775651403420873	26.572146060111713	23.501244218833456
46	23.534782230650432	26.03895377965112	26.046510183548154	24.37975380615029
47	23.70619873549274	25.848490996493002	26.523081190972064	23.922229077042196
48	23.87854685177441	26.837551807947268	25.578116654314186	23.705784685964133
49	22.90956744245747	26.584049984059092	26.593883660363453	23.912498913119986
50	22.602342692232842	26.887755313290572	26.062865139928036	24.44703685454854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	13.0
2	15.0
3	32.0
4	49.0
5	66.0
6	83.0
7	83.0
8	83.0
9	121.0
10	159.0
11	222.0
12	285.0
13	580.5
14	876.0
15	1204.5
16	1533.0
17	2274.0
18	3015.0
19	3430.5
20	3846.0
21	4983.0
22	6120.0
23	6590.5
24	7061.0
25	9362.0
26	11663.0
27	15479.5
28	19296.0
29	21589.0
30	23882.0
31	24574.0
32	25266.0
33	28776.5
34	32287.0
35	37551.0
36	42815.0
37	48817.5
38	54820.0
39	55501.0
40	56182.0
41	58964.0
42	61746.0
43	58427.0
44	55108.0
45	57845.0
46	60582.0
47	62029.5
48	63477.0
49	62967.0
50	62457.0
51	59038.0
52	55619.0
53	53434.0
54	51249.0
55	51628.0
56	52007.0
57	51313.5
58	50620.0
59	45976.5
60	41333.0
61	36276.0
62	31219.0
63	27577.5
64	23936.0
65	21289.0
66	18642.0
67	16451.5
68	14261.0
69	13277.5
70	12294.0
71	9743.0
72	7192.0
73	6476.0
74	5760.0
75	4299.5
76	2839.0
77	2524.0
78	2209.0
79	1949.0
80	1689.0
81	1350.5
82	1012.0
83	859.5
84	707.0
85	576.5
86	446.0
87	331.5
88	217.0
89	138.0
90	59.0
91	40.5
92	22.0
93	16.5
94	11.0
95	6.0
96	1.0
97	3.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012835535386742963
2	0.0038299581395926582
3	5.175619107557646E-4
4	3.105371464534588E-4
5	0.0
6	1.0351238215115293E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	966068.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.16017649966357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3875029444177	42.7341384489247
2	12.388126231550748	13.171099539386995
3	3.314099395081886	5.28534326339944
4	1.285298271330954	2.7330673183466394
5	0.6261083399925061	1.66420149309565
6	0.37714172124153433	1.2029352279952128
7	0.24181633828338256	0.8998499456552878
8	0.17730815607502282	0.7540586297422487
9	0.12946733697464596	0.6194255836062239
>10	0.8134923649482553	8.477027288710996
>50	0.12179433281437135	4.563202585435055
>100	0.12609645080112294	13.606885713619423
>500	0.010769106780574078	3.7241880828662746
>1k	9.790097073249162E-4	0.5645768792159276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1230	0.12732023004591808	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1067	0.11044771175528016	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1065	0.11024068699097786	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1059	0.10961961269807094	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1003	0.10382291929760638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.0351238215115292E-4	0.0
6	0.0	0.0	0.0	1.0351238215115292E-4	0.0
7	0.0	0.0	0.0	1.0351238215115292E-4	0.0
8	0.0	0.0	0.0	1.0351238215115292E-4	0.0
9	0.0	0.0	0.0	1.0351238215115292E-4	0.0
10	0.0	0.0	0.0	1.0351238215115292E-4	0.0
11	0.0	0.0	0.0	1.0351238215115292E-4	0.0
12	0.0	0.0	0.0	1.0351238215115292E-4	0.0
13	0.0	0.0	0.0	1.0351238215115292E-4	0.0
14	0.0	0.0	0.0	1.0351238215115292E-4	0.0
15	0.0	0.0	0.0	1.0351238215115292E-4	0.0
16	0.0	0.0	0.0	1.0351238215115292E-4	0.0
17	0.0	0.0	0.0	1.0351238215115292E-4	0.0
18	0.0	0.0	0.0	2.0702476430230584E-4	0.0
19	0.0	0.0	0.0	2.0702476430230584E-4	0.0
20	0.0	0.0	0.0	2.0702476430230584E-4	0.0
21	0.0	0.0	0.0	4.140495286046117E-4	0.0
22	0.0	0.0	0.0	5.175619107557646E-4	0.0
23	0.0	0.0	0.0	0.001345660967964988	0.0
24	0.0	0.0	0.0	0.0021737600251742115	0.0
25	0.0	0.0	0.0	0.0024842971716276703	0.0
26	0.0	0.0	0.0	0.0038299581395926582	0.0
27	0.0	0.0	0.0	0.005072106725406493	0.0
28	0.0	0.0	0.0	0.009109089629301457	0.0
29	0.0	0.0	0.0	0.01635495637988216	0.0
30	0.0	0.0	0.0	0.03126073940964818	0.0
31	0.0	0.0	0.0	0.07359730370946972	0.0
32	0.0	0.0	0.0	0.12338675952417429	0.0
33	0.0	0.0	0.0	0.16934625719928617	0.0
34	0.0	0.0	0.0	0.2284518274075945	0.0
35	0.0	0.0	0.0	0.2885925214374143	0.0
36	0.0	0.0	0.0	0.38661874733455615	0.0
37	0.0	0.0	0.0	0.5328817433141353	0.0
38	0.0	0.0	0.0	0.7165127092502805	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	100	4.622052E-8	21.999794	14
ACTGCGT	50	0.0025802073	21.999794	8
ACGGTAA	50	0.0025802073	21.999794	42
ATAACGC	320	0.0	19.938345	3
GTACTAA	80	8.9797E-5	19.25181	1
TCTAGAC	115	2.019442E-7	19.131245	3
TAACGCC	325	0.0	18.954647	4
ACCGACT	70	8.1222237E-4	18.856964	41
CGAATGC	350	0.0	18.856964	43
TTAACGG	410	0.0	18.780312	35
AATAACG	345	0.0	18.494493	2
CTAGACA	155	7.203198E-10	18.452394	4
AACGCCG	335	0.0	18.38884	5
CGCAATA	335	0.0	18.387888	36
ATACGAA	335	0.0	18.387888	40
AACAACG	60	0.007411454	18.33316	7
CCGGATC	60	0.007411454	18.33316	27
ATCGTTT	340	0.0	18.117477	29
GTATTAG	525	0.0	18.020744	1
CATCGTT	330	0.0	17.999832	28
>>END_MODULE