FastQCFastQC Report
Thu 2 Feb 2017
SRR4062387_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062387_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056445
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG60330.5710661700325147No Hit
TCCATGTACTCTGCGTTGATACCAC48290.45709904443676674No Hit
GAGTACATGGAAGCAGTGGTATCAA26560.2514092072942747No Hit
CATGTACTCTGCGTTGATACCACTG22150.20966543454699485No Hit
GTATCAACGCAGAGTACTTTTTTTT20800.19688672860395No Hit
GCTTCCATGTACTCTGCGTTGATAC16020.15164064385746537No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14360.1359275684015732No Hit
CCCATGTACTCTGCGTTGATACCAC14180.13422374094250056No Hit
TATCAACGCAGAGTACTTTTTTTTT14070.13318251305084505No Hit
GCGTTGATACCACTGCTTCCATGTA14040.13289854180766628No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13750.1301534864569381No Hit
CATGGAAGCAGTGGTATCAACGCAG12920.12229694872899204No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12880.12191832040475369No Hit
GCGCAAGACGGACCAGAGCGAAAGC12340.11680683802753573No Hit
GAATAGGACCGCGGTTCTATTTTGT12120.11472438224422474No Hit
GTACATGGGAAGCAGTGGTATCAAC11920.11283124062303292No Hit
GAATAACGCCGCCGCATCGCCAGTC11580.10961289986700681No Hit
GGGTAGGCACACGCTGAGCCAGTCA11570.10951824278594721No Hit
GAACTACGACGGTATCTGATCGTCT11520.10904495738064926No Hit
GTATCTGATCGTCTTCGAACCTCCG10600.10033650592316684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGC2050.013.90472916
TTAGGCA551.9604152E-413.81718354
ACGGTAT2150.013.6966849
ATCGCCA2450.013.57366416
GCATCGC2450.013.57173314
CGATAAC801.9982272E-613.06217410
CCGATAA801.9992913E-613.0615559
GCCGGTT957.380186E-812.99967511
GTCTAGA957.426206E-812.9935181
ATTACCG2200.012.95545115
GTTATAT1102.763045E-912.9480871
CGCATCG2500.012.91967913
CGGTTCT1850.012.83873512
GGTTCTA1850.012.83751813
GGCGAGA2700.012.67055419
TCCAACG1650.012.67055318
TATACTA905.404181E-712.665755
CGTAGGG1051.9965228E-812.663952
GGGTATA604.1073372E-412.660351
ACTGTTC3350.012.4778928
Produced by FastQC (version 0.11.5)