##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062387_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056445
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162760011169535	32.0	32.0	32.0	32.0	32.0
2	30.77151768430917	32.0	32.0	32.0	32.0	32.0
3	30.768570062804972	32.0	32.0	32.0	32.0	32.0
4	30.85521442195287	32.0	32.0	32.0	32.0	32.0
5	30.608411228222955	32.0	32.0	32.0	32.0	32.0
6	34.27387417234215	36.0	36.0	36.0	32.0	36.0
7	34.15774886529824	36.0	36.0	36.0	32.0	36.0
8	34.1341518015609	36.0	36.0	36.0	32.0	36.0
9	34.33334059037622	36.0	36.0	36.0	32.0	36.0
10	33.948810397133784	36.0	36.0	36.0	32.0	36.0
11	34.313453137645595	36.0	36.0	36.0	32.0	36.0
12	34.07486712512246	36.0	36.0	36.0	32.0	36.0
13	34.20850399216239	36.0	36.0	36.0	32.0	36.0
14	34.09834397436686	36.0	36.0	36.0	32.0	36.0
15	34.026429203602646	36.0	36.0	36.0	32.0	36.0
16	34.02578269573901	36.0	36.0	36.0	32.0	36.0
17	33.93855524897179	36.0	36.0	36.0	32.0	36.0
18	33.96297393617273	36.0	36.0	36.0	32.0	36.0
19	33.94619312884249	36.0	36.0	36.0	32.0	36.0
20	33.93453705588081	36.0	36.0	36.0	32.0	36.0
21	33.89751477833678	36.0	36.0	36.0	32.0	36.0
22	33.85756759698801	36.0	36.0	36.0	32.0	36.0
23	33.79079459886695	36.0	36.0	36.0	32.0	36.0
24	33.76045037839168	36.0	36.0	36.0	32.0	36.0
25	33.25823398283867	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	42.0
5	97.0
6	166.0
7	50.0
8	165.0
9	165.0
10	124.0
11	37.0
12	72.0
13	68.0
14	157.0
15	231.0
16	355.0
17	463.0
18	651.0
19	956.0
20	1367.0
21	2165.0
22	3288.0
23	4874.0
24	6685.0
25	9729.0
26	13305.0
27	17078.0
28	23139.0
29	30936.0
30	39686.0
31	54379.0
32	77596.0
33	109829.0
34	237467.0
35	421115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.71798736041743	16.85829884395909	10.855443767489238	25.568270028134243
2	16.447243867899523	19.629442412401268	37.21223239505949	26.711081324639725
3	19.7808574484802	23.65348024877176	27.906549664423174	28.659112638324864
4	12.36996769783655	14.875562826382499	35.45067766379242	37.30379181198853
5	14.356339547211046	36.68065870196965	33.66525357526014	15.29774817555917
6	35.1824651618677	34.487927989355605	16.69341970065012	13.636187148126577
7	30.41270069798523	30.158190410067505	20.5317910533998	18.897317838547465
8	28.546321701430998	33.11510884065001	19.38572489691972	18.95284456099927
9	26.925030128929077	14.357476901154373	18.34245412444195	40.3750388454746
10	15.72304633489669	26.759343409694193	31.77010431108908	25.747505944320036
11	38.08293641692996	20.805748196107345	21.529200601202785	19.58211478575991
12	24.254481451959155	23.453871824739757	29.09965300913398	23.191993714167104
13	30.070555010637744	19.09240384569823	25.252060026250824	25.584981117413204
14	23.33496026650113	19.722031360321466	24.678459562813046	32.26454881036435
15	25.47378196411396	27.264577815471654	22.592380257465994	24.6692599629484
16	26.08495831493404	25.471859026963102	24.270582415895475	24.172600242207384
17	23.81115749669483	25.956035312012816	25.947695384134235	24.28511180715812
18	25.24334047953476	24.184963406520293	27.24351866068433	23.328177453260622
19	25.34227035306093	25.831139991299295	24.797102039926568	24.029487615713208
20	25.049042556619277	24.31212483628789	26.001652757850124	24.637179849242706
21	26.217266446152532	24.733724445792383	23.78538730558899	25.263621802466098
22	25.121291807518105	25.104038889215406	24.84126367198979	24.9334056312767
23	23.820108207300013	24.615454610942813	25.365114330453114	26.199322851304064
24	24.26715624016651	25.83530161089143	25.044262549901998	24.85327959904006
25	25.02523488584354	24.768384028994767	24.881834389012468	25.324546696149223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	118.0
2	313.0
3	508.0
4	508.0
5	508.0
6	1411.5
7	2315.0
8	2315.0
9	2315.0
10	2388.0
11	2461.0
12	2461.0
13	2461.0
14	2595.0
15	2729.0
16	2729.0
17	2729.0
18	4457.5
19	6186.0
20	6186.0
21	6186.0
22	10045.0
23	13904.0
24	13904.0
25	13904.0
26	21746.0
27	29588.0
28	29588.0
29	29588.0
30	38671.0
31	47754.0
32	47754.0
33	47754.0
34	56312.5
35	64871.0
36	64871.0
37	64871.0
38	73346.5
39	81822.0
40	81822.0
41	81822.0
42	94637.5
43	107453.0
44	107453.0
45	107453.0
46	130570.0
47	153687.0
48	153687.0
49	153687.0
50	147367.0
51	141047.0
52	141047.0
53	141047.0
54	128872.5
55	116698.0
56	116698.0
57	116698.0
58	108391.0
59	100084.0
60	100084.0
61	100084.0
62	88745.0
63	77406.0
64	77406.0
65	77406.0
66	64215.5
67	51025.0
68	51025.0
69	51025.0
70	38612.0
71	26199.0
72	26199.0
73	26199.0
74	20407.0
75	14615.0
76	14615.0
77	14615.0
78	11954.5
79	9294.0
80	9294.0
81	9294.0
82	6489.5
83	3685.0
84	3685.0
85	3685.0
86	2716.0
87	1747.0
88	1747.0
89	1747.0
90	1180.5
91	614.0
92	614.0
93	614.0
94	376.5
95	139.0
96	139.0
97	139.0
98	317.5
99	496.0
100	496.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007667223565826901
2	0.003502311999204881
3	0.005206139458277525
4	0.01599704669907094
5	0.03483380582992962
6	0.04638196971919977
7	0.06550270009323722
8	0.09058682657402893
9	0.09285859651945913
10	0.11699615218965494
11	0.1161442384601186
12	0.13005882937587854
13	0.11737478051389329
14	0.12210763456687286
15	0.10980221402912599
16	0.10895030029958966
17	0.12116106375627694
18	0.12674583153879285
19	0.12854431607892508
20	0.11728012343283371
21	0.12636720321455447
22	0.14681313272342622
23	0.1356435971583944
24	0.13034280061905731
25	0.12854431607892508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1056445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.97952797003119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4999237872109	39.23389136568224
2	12.622117878387003	12.61694987087748
3	3.822067211649522	5.730753453239292
4	1.6252796566415726	3.249228402329607
5	0.8643694377848161	2.1600388246103175
6	0.5136141433996483	1.54021154675078
7	0.3416354688095106	1.195234562824376
8	0.252975574382604	1.011487983647205
9	0.18149180008241933	0.8163787048695528
>10	1.0460481048704757	9.686019030413044
>50	0.10462982561177342	3.6849142530414887
>100	0.11068480882502167	11.588065602857275
>500	0.011182197979291549	3.8673616329839366
>1k	0.0037905755862005248	3.047985285828597
>5k	1.8952877931002624E-4	0.5714794800448744
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6033	0.5710661700325147	No Hit
TCCATGTACTCTGCGTTGATACCAC	4829	0.45709904443676674	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2656	0.2514092072942747	No Hit
CATGTACTCTGCGTTGATACCACTG	2215	0.20966543454699485	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2080	0.19688672860395	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1602	0.15164064385746537	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1436	0.1359275684015732	No Hit
CCCATGTACTCTGCGTTGATACCAC	1418	0.13422374094250056	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1407	0.13318251305084505	No Hit
GCGTTGATACCACTGCTTCCATGTA	1404	0.13289854180766628	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1375	0.1301534864569381	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1292	0.12229694872899204	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1288	0.12191832040475369	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1234	0.11680683802753573	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1212	0.11472438224422474	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1192	0.11283124062303292	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1158	0.10961289986700681	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1157	0.10951824278594721	No Hit
GAACTACGACGGTATCTGATCGTCT	1152	0.10904495738064926	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1060	0.10033650592316684	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.465708105959137E-5
2	0.0	0.0	0.0	0.0	9.465708105959137E-5
3	0.0	0.0	0.0	0.0	9.465708105959137E-5
4	0.0	0.0	0.0	0.0	9.465708105959137E-5
5	0.0	0.0	0.0	0.0	9.465708105959137E-5
6	0.0	0.0	0.0	0.0	1.8931416211918274E-4
7	0.0	0.0	0.0	0.0	1.8931416211918274E-4
8	0.0	0.0	0.0	0.0	1.8931416211918274E-4
9	0.0	0.0	0.0	9.465708105959137E-5	1.8931416211918274E-4
10	0.0	0.0	0.0	9.465708105959137E-5	3.786283242383655E-4
11	0.0	0.0	0.0	9.465708105959137E-5	3.786283242383655E-4
12	0.0	0.0	0.0	9.465708105959137E-5	5.679424863575482E-4
13	0.0	0.0	0.0	9.465708105959137E-5	6.625995674171396E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGC	205	0.0	13.904729	16
TTAGGCA	55	1.9604152E-4	13.8171835	4
ACGGTAT	215	0.0	13.696684	9
ATCGCCA	245	0.0	13.573664	16
GCATCGC	245	0.0	13.571733	14
CGATAAC	80	1.9982272E-6	13.062174	10
CCGATAA	80	1.9992913E-6	13.061555	9
GCCGGTT	95	7.380186E-8	12.999675	11
GTCTAGA	95	7.426206E-8	12.993518	1
ATTACCG	220	0.0	12.955451	15
GTTATAT	110	2.763045E-9	12.948087	1
CGCATCG	250	0.0	12.919679	13
CGGTTCT	185	0.0	12.838735	12
GGTTCTA	185	0.0	12.837518	13
GGCGAGA	270	0.0	12.670554	19
TCCAACG	165	0.0	12.670553	18
TATACTA	90	5.404181E-7	12.66575	5
CGTAGGG	105	1.9965228E-8	12.66395	2
GGGTATA	60	4.1073372E-4	12.66035	1
ACTGTTC	335	0.0	12.477892	8
>>END_MODULE