FastQCFastQC Report
Thu 2 Feb 2017
SRR4062387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056445
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23570.22310674005745684No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22090.20909749206063735No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14770.13980850872501643No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12680.12002517878356184No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12440.11775340883813167No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11790.11160069856925821No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11590.10970755694806639No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11030.10440676040872929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA250.002352683635.1999244
TATACCG451.0370677E-634.222155
ATCGTGT703.2149124E-521.9999528
GTATAGT1001.1221655E-619.8018321
CTATACC1452.9649527E-1019.7240964
TACCGTC2800.019.6428157
ACCGTCG2700.019.5555138
CGTCGTA2900.018.96547710
TGGTATA2900.018.96547744
ATACCGT3250.018.9538046
TCTATAC1404.0799932E-918.8571033
TAGACCG600.0074115918.3332945
CCGTCGT2900.018.2068589
CGAGCCG5100.018.11760915
TAGGACG5100.018.1176094
TCCTACA5650.017.9114672
GTATTAG3450.017.8567261
GTCCTAC5550.017.8394891
CGTCTTA1852.1827873E-1117.83780115
TTAGGAC3850.017.7142493
Produced by FastQC (version 0.11.5)