##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056445
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241627344537577	32.0	32.0	32.0	32.0	32.0
2	31.376345195443207	32.0	32.0	32.0	32.0	32.0
3	31.490992905451776	32.0	32.0	32.0	32.0	32.0
4	31.606563522000673	32.0	32.0	32.0	32.0	32.0
5	31.53399940366039	32.0	32.0	32.0	32.0	32.0
6	35.139941028638496	36.0	36.0	36.0	36.0	36.0
7	35.15487318317565	36.0	36.0	36.0	36.0	36.0
8	35.08742244035421	36.0	36.0	36.0	36.0	36.0
9	35.22169634954967	36.0	36.0	36.0	36.0	36.0
10	35.10034313191884	36.0	36.0	36.0	36.0	36.0
11	35.237918680101664	36.0	36.0	36.0	36.0	36.0
12	35.13807249785838	36.0	36.0	36.0	36.0	36.0
13	35.18881815901443	36.0	36.0	36.0	36.0	36.0
14	35.14961876860603	36.0	36.0	36.0	36.0	36.0
15	35.10863319907804	36.0	36.0	36.0	36.0	36.0
16	35.116753830062144	36.0	36.0	36.0	36.0	36.0
17	35.08585491909186	36.0	36.0	36.0	36.0	36.0
18	35.10906862165091	36.0	36.0	36.0	36.0	36.0
19	35.07467686438953	36.0	36.0	36.0	36.0	36.0
20	35.073281619014715	36.0	36.0	36.0	36.0	36.0
21	35.05658505648661	36.0	36.0	36.0	36.0	36.0
22	35.03234148488563	36.0	36.0	36.0	36.0	36.0
23	34.97996582879374	36.0	36.0	36.0	36.0	36.0
24	34.9483740279901	36.0	36.0	36.0	32.0	36.0
25	34.92058176242019	36.0	36.0	36.0	32.0	36.0
26	34.8644983884632	36.0	36.0	36.0	32.0	36.0
27	34.83641552565444	36.0	36.0	36.0	32.0	36.0
28	34.81605384094771	36.0	36.0	36.0	32.0	36.0
29	34.77841534580598	36.0	36.0	36.0	32.0	36.0
30	34.755115505303166	36.0	36.0	36.0	32.0	36.0
31	34.74730440297412	36.0	36.0	36.0	32.0	36.0
32	34.70963372442484	36.0	36.0	36.0	32.0	36.0
33	34.67718906332085	36.0	36.0	36.0	32.0	36.0
34	34.6624623146496	36.0	36.0	36.0	32.0	36.0
35	34.612110426950764	36.0	36.0	36.0	32.0	36.0
36	34.57473886477763	36.0	36.0	36.0	32.0	36.0
37	34.54726559357089	36.0	36.0	36.0	32.0	36.0
38	34.488554539043676	36.0	36.0	36.0	32.0	36.0
39	34.41507414015874	36.0	36.0	36.0	32.0	36.0
40	34.3799980122013	36.0	36.0	36.0	32.0	36.0
41	34.366647577488656	36.0	36.0	36.0	32.0	36.0
42	34.277502378259165	36.0	36.0	36.0	32.0	36.0
43	34.3030105684631	36.0	36.0	36.0	32.0	36.0
44	34.26847777215094	36.0	36.0	36.0	32.0	36.0
45	34.16356932921259	36.0	36.0	36.0	32.0	36.0
46	34.22061347254235	36.0	36.0	36.0	32.0	36.0
47	34.166933441873454	36.0	36.0	36.0	32.0	36.0
48	34.140650010175634	36.0	36.0	36.0	32.0	36.0
49	34.162541353312285	36.0	36.0	36.0	32.0	36.0
50	33.61765165247599	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	4.0
21	29.0
22	75.0
23	252.0
24	739.0
25	1864.0
26	4024.0
27	7399.0
28	12517.0
29	18452.0
30	26125.0
31	37203.0
32	55539.0
33	84936.0
34	184810.0
35	622473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.161733130867134	17.45712424785195	11.376984743086297	26.00415787819462
2	15.966213939258548	20.11563670234719	36.85863091293927	27.059518445454994
3	19.199688765749844	23.848513733400605	28.3183411441645	28.63345635668505
4	12.209544471831919	15.37573217321505	35.60548405783931	36.80923929711371
5	14.178494857754071	36.90897301799904	33.4346795147878	15.477852609459083
6	35.330894950768524	34.834415270938756	16.36499255043836	13.469697227854354
7	30.404422378827107	30.21747464373441	20.517206290909606	18.86089668652888
8	28.45259336737833	33.35706070831893	19.168910828296788	19.021435096005945
9	26.99373843408791	14.115737213011561	18.16062360085002	40.729900752050504
10	15.764474250907526	26.85866277941587	31.739087221767342	25.637775747909263
11	38.21164376754114	20.701219656489453	21.569793032292264	19.517343543677143
12	24.235147120768236	23.486977552073228	29.051299405080243	23.22657592207829
13	30.300110275499435	19.023233580546076	25.239553407891563	25.43710273606293
14	23.417309940413368	19.55974991599184	24.61169298922329	32.4112471543715
15	25.586376952893904	27.25082706624576	22.504531707755728	24.65826427310461
16	26.242255867555812	25.360903785809956	24.215269133745725	24.181571212888507
17	24.01109380990018	25.946736460487767	25.603604541646753	24.4385651879653
18	25.436629450657627	24.061546034104946	27.06274344618035	23.439081069057075
19	25.46417466124597	25.657085792445418	24.75595038075811	24.1227891655505
20	25.21219751146534	24.092498899611435	26.032685090089874	24.662618498833353
21	26.412070670976718	24.691867536880764	23.76384951417253	25.132212277969984
22	25.197714978063225	24.924061356719942	24.84596926484578	25.032254400371055
23	23.963670612289327	24.444149955747815	25.29294000160917	26.299239430353687
24	24.427584966562385	25.77313537382448	24.955771478874905	24.84350818073823
25	25.268329160533675	24.45626606212344	24.853920459654784	25.421484317688098
26	23.939154428294895	25.339795256733666	25.80683329468169	24.914217020289744
27	25.33695554430188	25.189669126173158	25.158621603585612	24.314753725939354
28	24.38546256549087	24.502648031842643	25.488880159402527	25.623009243263965
29	24.07905759410097	24.982180804490532	25.79869278571057	25.14006881569793
30	24.717803577091093	24.907780338777695	25.985072578316903	24.389343505814313
31	24.771568799132943	24.804509463341677	24.864806023976637	25.559115713548742
32	24.43771327423576	25.169412510826405	24.957664620496097	25.435209594441737
33	24.058895635835277	24.515332080704628	25.678572949845947	25.74719933361415
34	24.495075465357875	25.31225004614533	26.084651827591593	24.108022660905206
35	25.54311866684967	24.844738722792005	25.67242023957707	23.93972237078125
36	24.18147655580745	25.73423131350899	25.21711967968044	24.86717245100313
37	25.476764053026894	25.09605327300522	25.135619932888133	24.29156274107975
38	24.580550809554687	25.06358589420178	25.021652807292384	25.334210488951154
39	24.997420594541126	24.49147849627761	25.30704390668705	25.204057002494213
40	25.81175546289679	24.91554221942458	24.99003734221848	24.282664975460154
41	24.040910790433955	25.02051692231967	26.24149861090734	24.697073676339045
42	26.02321938198392	25.69845093686846	25.08280128165688	23.195528399490744
43	24.661577270941695	24.317309467127963	25.370937436402276	25.65017582552807
44	24.19302471969672	24.958895162549872	25.864100828722748	24.983979289030664
45	24.569286616908595	25.256591682482288	25.3045828225795	24.86953887802962
46	24.20213470851271	24.62051940282684	25.919689070125816	25.25765681853463
47	25.057717155176086	24.386977078787822	26.122420002934373	24.432885763101723
48	25.211156283573683	26.092603022400596	24.340500451987563	24.355740242038156
49	24.11180894414759	25.186829413741368	25.903856802767773	24.79750483934327
50	24.08114004988428	26.33662897737222	24.23192878001221	25.350302192731284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	14.0
2	20.0
3	61.0
4	102.0
5	143.5
6	185.0
7	162.5
8	140.0
9	196.0
10	252.0
11	355.5
12	459.0
13	852.0
14	1245.0
15	1621.0
16	1997.0
17	2311.0
18	2625.0
19	2890.0
20	3155.0
21	3766.0
22	4377.0
23	4686.0
24	4995.0
25	5933.5
26	6872.0
27	9175.0
28	11478.0
29	13290.5
30	15103.0
31	16098.0
32	17093.0
33	20087.5
34	23082.0
35	27135.5
36	31189.0
37	36121.5
38	41054.0
39	43321.5
40	45589.0
41	49002.0
42	52415.0
43	53646.0
44	54877.0
45	62995.0
46	71113.0
47	77854.5
48	84596.0
49	84233.0
50	83870.0
51	78530.0
52	73190.0
53	71234.5
54	69279.0
55	70737.5
56	72196.0
57	70602.0
58	69008.0
59	63575.5
60	58143.0
61	51524.5
62	44906.0
63	38931.5
64	32957.0
65	28474.0
66	23991.0
67	20655.0
68	17319.0
69	16022.0
70	14725.0
71	11317.5
72	7910.0
73	7102.0
74	6294.0
75	4457.0
76	2620.0
77	2372.0
78	2124.0
79	1824.0
80	1524.0
81	1233.5
82	943.0
83	816.0
84	689.0
85	567.5
86	446.0
87	325.0
88	204.0
89	131.0
90	58.0
91	38.0
92	18.0
93	11.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012968020105164017
2	0.0031236836749665153
3	5.679424863575482E-4
4	9.465708105959137E-5
5	0.0
6	6.625995674171396E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.465708105959137E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1056445.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.168002315403996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94843431222426	42.76478972622085
2	12.704972287780356	13.7640593660326
3	3.673999862203198	5.970396991278503
4	1.5020756126249837	3.25457741050328
5	0.8277171791410384	2.2417893038105716
6	0.49041891934465	1.5939007895147357
7	0.3241852322616769	1.2292326488239211
8	0.23299449532346414	1.0096677089726234
9	0.1765768933524031	0.8608335811163834
>10	0.9259013577945371	9.280551844996092
>50	0.09082618356678368	3.4625748458121657
>100	0.092042277548148	10.310485002665466
>500	0.008095496328283098	2.918043739694548
>1k	0.0017598905061484995	1.3390970405582918
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2357	0.22310674005745684	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2209	0.20909749206063735	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1477	0.13980850872501643	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1268	0.12002517878356184	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1244	0.11775340883813167	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1179	0.11160069856925821	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1159	0.10970755694806639	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1103	0.10440676040872929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.465708105959137E-5
2	0.0	0.0	0.0	0.0	9.465708105959137E-5
3	0.0	0.0	0.0	0.0	9.465708105959137E-5
4	0.0	0.0	0.0	0.0	9.465708105959137E-5
5	0.0	0.0	0.0	0.0	9.465708105959137E-5
6	0.0	0.0	0.0	0.0	9.465708105959137E-5
7	0.0	0.0	0.0	0.0	9.465708105959137E-5
8	0.0	0.0	0.0	0.0	9.465708105959137E-5
9	0.0	0.0	0.0	0.0	9.465708105959137E-5
10	0.0	0.0	0.0	0.0	1.8931416211918274E-4
11	0.0	0.0	0.0	9.465708105959137E-5	1.8931416211918274E-4
12	0.0	0.0	0.0	1.8931416211918274E-4	6.625995674171396E-4
13	0.0	0.0	0.0	1.8931416211918274E-4	0.0010412278916555051
14	0.0	0.0	0.0	1.8931416211918274E-4	0.0010412278916555051
15	0.0	0.0	0.0	2.839712431787741E-4	0.0011358849727150964
16	0.0	0.0	0.0	2.839712431787741E-4	0.0013251991348342792
17	0.0	0.0	0.0	4.732854052979568E-4	0.0013251991348342792
18	0.0	0.0	0.0	6.625995674171396E-4	0.001514513296953462
19	0.0	0.0	0.0	8.519137295363222E-4	0.001514513296953462
20	0.0	0.0	0.0	0.0011358849727150964	0.001798484540132236
21	0.0	0.0	0.0	0.001514513296953462	0.0018931416211918272
22	0.0	0.0	0.0	0.0021771128643706013	0.0018931416211918272
23	0.0	0.0	0.0	0.003407654918145289	0.0018931416211918272
24	0.0	0.0	0.0	0.005679424863575482	0.0019877987022514187
25	0.0	0.0	0.0	0.007193938160528944	0.0019877987022514187
26	0.0	0.0	0.0	0.009181736862780362	0.0021771128643706013
27	0.0	0.0	0.0	0.011264192646091372	0.0021771128643706013
28	0.0	0.0	0.0	0.016186360861190123	0.0021771128643706013
29	0.0	0.0	0.0	0.025178783561851305	0.0022717699454301928
30	0.0	0.0	0.0	0.039850631126087964	0.0022717699454301928
31	0.0	0.0	0.0	0.07193938160528944	0.0023664270264897843
32	0.0	0.0	0.0	0.11235795521773495	0.0023664270264897843
33	0.0	0.0	0.0	0.1489902455877968	0.002555741188608967
34	0.0	0.0	0.0	0.19698138568500964	0.0026503982696685583
35	0.0	0.0	0.0	0.2505572935647383	0.00274505535072815
36	0.0	0.0	0.0	0.3288386996010204	0.00274505535072815
37	0.0	0.0	0.0	0.43996611276498065	0.00274505535072815
38	0.0	0.0	0.0	0.5810998206248313	0.002839712431787741
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCA	25	0.0023526836	35.199924	4
TATACCG	45	1.0370677E-6	34.22215	5
ATCGTGT	70	3.2149124E-5	21.999952	8
GTATAGT	100	1.1221655E-6	19.801832	1
CTATACC	145	2.9649527E-10	19.724096	4
TACCGTC	280	0.0	19.642815	7
ACCGTCG	270	0.0	19.555513	8
CGTCGTA	290	0.0	18.965477	10
TGGTATA	290	0.0	18.965477	44
ATACCGT	325	0.0	18.953804	6
TCTATAC	140	4.0799932E-9	18.857103	3
TAGACCG	60	0.00741159	18.333294	5
CCGTCGT	290	0.0	18.206858	9
CGAGCCG	510	0.0	18.117609	15
TAGGACG	510	0.0	18.117609	4
TCCTACA	565	0.0	17.911467	2
GTATTAG	345	0.0	17.856726	1
GTCCTAC	555	0.0	17.839489	1
CGTCTTA	185	2.1827873E-11	17.837801	15
TTAGGAC	385	0.0	17.714249	3
>>END_MODULE