FastQCFastQC Report
Thu 2 Feb 2017
SRR4062386_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062386_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615627
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52670.2013666321688834No Hit
TATCAACGCAGAGTACTTTTTTTTT34480.13182307722010822No Hit
GTCCTAAAGTGTGTATTTCTCATTT30570.11687446260495093No Hit
GCGCAAGACGGACCAGAGCGAAAGC28220.10789000113548301No Hit
GTCCTACAGTGGACATTTCTAAATT27980.10697243911306925No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26890.10280517826127349No Hit
GTCTTGCGCCGGTCCAAGAATTTCA26730.10219347024633099No Hit
CTGTAGGACGTGGAATATGGCAAGA26630.10181115273699194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGG653.3687138E-614.61769718
AATTGCG400.00527751814.25116315
AAGACGG4900.013.1827315
ACGGTAT4250.012.7422169
CGCATCG4150.012.59114913
ATCGCCA4450.012.38398216
CAAGACG5200.012.2392744
GCATCGC4450.011.74253114
CGAGCCG4350.011.57565715
CGCGCTA1751.8189894E-1211.40180416
ACGCGCG1751.8189894E-1211.40071313
CGAACGA2100.011.31131416
GCGCAAG5650.011.2599381
CCGTCAA3500.011.13033318
TCGCCAG4650.011.03421517
CGTCAAT3450.011.01623519
AACCGCG1900.011.0008977
CATCGCC4950.010.94028815
CCGCATC5100.010.80501112
AGGCCCG4850.010.774281510
Produced by FastQC (version 0.11.5)