##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062386_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2615627
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221494884400567	32.0	32.0	32.0	32.0	32.0
2	30.894742637233826	32.0	32.0	32.0	32.0	32.0
3	30.8780185401053	32.0	32.0	32.0	32.0	32.0
4	30.945964007865037	32.0	32.0	32.0	32.0	32.0
5	30.765709713196873	32.0	32.0	32.0	32.0	32.0
6	34.41751098302625	36.0	36.0	36.0	32.0	36.0
7	34.31064253427572	36.0	36.0	36.0	32.0	36.0
8	34.30694667091294	36.0	36.0	36.0	32.0	36.0
9	34.4590440456533	36.0	36.0	36.0	32.0	36.0
10	34.14192237654681	36.0	36.0	36.0	32.0	36.0
11	34.43959134846062	36.0	36.0	36.0	32.0	36.0
12	34.2263105557482	36.0	36.0	36.0	32.0	36.0
13	34.34870835941057	36.0	36.0	36.0	32.0	36.0
14	34.25543588592716	36.0	36.0	36.0	32.0	36.0
15	34.20127564060166	36.0	36.0	36.0	32.0	36.0
16	34.20148706218433	36.0	36.0	36.0	32.0	36.0
17	34.13646288251345	36.0	36.0	36.0	32.0	36.0
18	34.14923190500786	36.0	36.0	36.0	32.0	36.0
19	34.133207448921425	36.0	36.0	36.0	32.0	36.0
20	34.12094690871443	36.0	36.0	36.0	32.0	36.0
21	34.10558042106157	36.0	36.0	36.0	32.0	36.0
22	34.05018070237079	36.0	36.0	36.0	32.0	36.0
23	34.029618519766004	36.0	36.0	36.0	32.0	36.0
24	33.998625950871435	36.0	36.0	36.0	32.0	36.0
25	33.55904683657111	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	13.0
4	107.0
5	228.0
6	460.0
7	107.0
8	361.0
9	487.0
10	339.0
11	96.0
12	205.0
13	179.0
14	415.0
15	578.0
16	871.0
17	1186.0
18	1558.0
19	2134.0
20	3270.0
21	4483.0
22	6777.0
23	10037.0
24	14566.0
25	20218.0
26	28744.0
27	37643.0
28	51065.0
29	68158.0
30	88667.0
31	122395.0
32	176374.0
33	256487.0
34	584764.0
35	1132651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72858231465434	17.80180982084173	11.33866101398755	26.130946850516384
2	16.527472762753025	19.813720782279425	37.889595096377946	25.769211358589594
3	19.339895507351383	23.544720980158633	28.30741408414086	28.80796942834913
4	12.642022968393796	15.17845739941864	35.95589095633833	36.223628675849234
5	14.477848434097949	36.574302661385225	33.664169112143654	15.283679792373173
6	34.15756905350399	35.29269567040057	16.81323826069395	13.736497015401492
7	30.155474033399432	30.400550599741305	20.734449249142177	18.709526117717093
8	27.626379782602207	33.700254929257326	19.571235163247326	19.10213012489314
9	27.235637638342492	14.35845821788847	19.05115802042035	39.3547461233487
10	16.189242922663173	26.640711902113463	31.594522322347917	25.575522852875455
11	37.11956527980175	21.10077976940323	22.757660561643736	19.02199438915129
12	24.88137851299151	23.589347993369294	28.92787001979258	22.601403473846613
13	29.430647333911853	19.68207292616854	25.404538239021786	25.482741500897827
14	22.878609180842705	20.3885342247977	25.21766958321897	31.51518701114063
15	24.61251451611298	28.424022836898217	22.20982510236874	24.753637544620062
16	25.010755453895467	25.911151530183403	24.89087615994317	24.187216855977965
17	23.477995575649764	26.467207250452752	25.749733670040243	24.305063503857237
18	24.283162491845854	24.867964081942663	27.48012378989407	23.368749636317418
19	25.394008860222563	25.06768438251783	25.525396190992467	24.012910566267145
20	25.463873682582665	24.78595377512581	25.586174874244133	24.163997668047386
21	25.967741688500418	24.50045249838831	24.73148699698794	24.800318816123337
22	25.41108269133571	25.31440014703405	25.149522905147727	24.124994256482516
23	23.68664571910538	25.07294445195276	26.11172092243289	25.128688906508966
24	24.46551518195684	25.28249863708477	25.973029592105508	24.278956588852886
25	24.22443472312028	25.2155810188402	25.974464488135528	24.58551976990399
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	344.0
1	344.0
2	868.0
3	1392.0
4	1392.0
5	1392.0
6	3378.0
7	5364.0
8	5364.0
9	5364.0
10	5953.0
11	6542.0
12	6542.0
13	6542.0
14	7645.5
15	8749.0
16	8749.0
17	8749.0
18	13893.0
19	19037.0
20	19037.0
21	19037.0
22	30701.0
23	42365.0
24	42365.0
25	42365.0
26	64160.0
27	85955.0
28	85955.0
29	85955.0
30	109039.0
31	132123.0
32	132123.0
33	132123.0
34	155830.5
35	179538.0
36	179538.0
37	179538.0
38	200627.0
39	221716.0
40	221716.0
41	221716.0
42	247579.0
43	273442.0
44	273442.0
45	273442.0
46	304533.5
47	335625.0
48	335625.0
49	335625.0
50	336960.5
51	338296.0
52	338296.0
53	338296.0
54	311782.5
55	285269.0
56	285269.0
57	285269.0
58	261176.5
59	237084.0
60	237084.0
61	237084.0
62	210301.5
63	183519.0
64	183519.0
65	183519.0
66	151794.0
67	120069.0
68	120069.0
69	120069.0
70	91930.0
71	63791.0
72	63791.0
73	63791.0
74	50036.0
75	36281.0
76	36281.0
77	36281.0
78	29536.0
79	22791.0
80	22791.0
81	22791.0
82	16002.5
83	9214.0
84	9214.0
85	9214.0
86	6755.0
87	4296.0
88	4296.0
89	4296.0
90	2856.5
91	1417.0
92	1417.0
93	1417.0
94	844.0
95	271.0
96	271.0
97	271.0
98	704.0
99	1137.0
100	1137.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008793302714798402
2	0.0034790893349854546
3	0.005046591123275605
4	0.014566297105818223
5	0.03578491887413611
6	0.046069259875356844
7	0.06690556413433567
8	0.08995930994748104
9	0.09737626962865882
10	0.1197800756759278
11	0.12188282197729264
12	0.13522570305322584
13	0.12249452999223513
14	0.1260118510781545
15	0.11519226556385906
16	0.11465702105078437
17	0.1248648985501373
18	0.13224362648038118
19	0.13392582352147306
20	0.12325916501091325
21	0.13239655348411683
22	0.15242999097348361
23	0.1417633324629238
24	0.1368314365924499
25	0.13381112826867134
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2615627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.49243502430121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31460852158914	39.54295282229925
2	13.02428905615162	13.15256137810906
3	3.7783737711223218	5.723378764077533
4	1.579485372043063	3.190082500788743
5	0.8146645453979992	2.056719831255518
6	0.49173150464584214	1.4897232628639232
7	0.33910730403055767	1.1985647460520223
8	0.23563781750828736	0.9518341751844277
9	0.1868481019386372	0.8490974081895583
>10	1.037078219022031	9.532184621164644
>50	0.09177135561720481	3.21182800555446
>100	0.0872775556177245	9.476482603171501
>500	0.013434352900268683	4.71717499828126
>1k	0.005616622116495502	4.705562853826064
>5k	7.590029887156084E-5	0.20185202918215628
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5267	0.2013666321688834	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3448	0.13182307722010822	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3057	0.11687446260495093	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2822	0.10789000113548301	No Hit
GTCCTACAGTGGACATTTCTAAATT	2798	0.10697243911306925	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2689	0.10280517826127349	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2673	0.10219347024633099	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2663	0.10181115273699194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.8231750933906095E-5	0.0
9	0.0	0.0	0.0	7.646350186781219E-5	0.0
10	0.0	0.0	0.0	7.646350186781219E-5	0.0
11	0.0	0.0	0.0	1.1469525280171829E-4	0.0
12	0.0	0.0	0.0	1.9115875466953048E-4	3.8231750933906095E-5
13	0.0	0.0	0.0	1.9115875466953048E-4	3.8231750933906095E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGG	65	3.3687138E-6	14.617697	18
AATTGCG	40	0.005277518	14.251163	15
AAGACGG	490	0.0	13.182731	5
ACGGTAT	425	0.0	12.742216	9
CGCATCG	415	0.0	12.591149	13
ATCGCCA	445	0.0	12.383982	16
CAAGACG	520	0.0	12.239274	4
GCATCGC	445	0.0	11.742531	14
CGAGCCG	435	0.0	11.575657	15
CGCGCTA	175	1.8189894E-12	11.401804	16
ACGCGCG	175	1.8189894E-12	11.400713	13
CGAACGA	210	0.0	11.311314	16
GCGCAAG	565	0.0	11.259938	1
CCGTCAA	350	0.0	11.130333	18
TCGCCAG	465	0.0	11.034215	17
CGTCAAT	345	0.0	11.016235	19
AACCGCG	190	0.0	11.000897	7
CATCGCC	495	0.0	10.940288	15
CCGCATC	510	0.0	10.805011	12
AGGCCCG	485	0.0	10.7742815	10
>>END_MODULE