FastQCFastQC Report
Thu 2 Feb 2017
SRR4062386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615627
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26680.10200231149166147No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26490.10127590822391726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA21900.020.1920192
GTCCTAC21300.019.9386981
GTATCAA47800.019.5650121
TCCTACA22600.018.9825232
GTAGGAC24150.018.8573633
TAGGACG20400.018.2257044
CTGTAGG23400.017.9612541
CGTCGTA6300.017.80939310
TTAGGAC13500.017.763173
TAGGACC19450.017.7585644
ACCGTCG6550.017.465528
AGGACGT20950.017.4321865
ATACCGT6950.017.4102766
GACGTGA11500.017.4085667
CCGTCGT6700.017.0745019
GGACGTG21100.016.8911936
CCTACAG23500.016.7576433
CTAGCGG7800.016.64090329
GTATCGA800.001989281716.499889
TACCGTC6950.016.460317
Produced by FastQC (version 0.11.5)