##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2615627
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30355589692261	32.0	32.0	32.0	32.0	32.0
2	31.427379744894818	32.0	32.0	32.0	32.0	32.0
3	31.50914254975958	32.0	32.0	32.0	32.0	32.0
4	31.604940230392177	32.0	32.0	32.0	32.0	32.0
5	31.54405654934744	32.0	32.0	32.0	32.0	32.0
6	35.14372270969829	36.0	36.0	36.0	36.0	36.0
7	35.151695559038046	36.0	36.0	36.0	36.0	36.0
8	35.09190339448247	36.0	36.0	36.0	36.0	36.0
9	35.21851930722538	36.0	36.0	36.0	36.0	36.0
10	35.101868882680904	36.0	36.0	36.0	36.0	36.0
11	35.23619996276228	36.0	36.0	36.0	36.0	36.0
12	35.14718688865041	36.0	36.0	36.0	36.0	36.0
13	35.189424180129656	36.0	36.0	36.0	36.0	36.0
14	35.150380386805914	36.0	36.0	36.0	36.0	36.0
15	35.10968154098425	36.0	36.0	36.0	36.0	36.0
16	35.12722838539287	36.0	36.0	36.0	36.0	36.0
17	35.09961397401082	36.0	36.0	36.0	36.0	36.0
18	35.11684540647424	36.0	36.0	36.0	36.0	36.0
19	35.09793751173237	36.0	36.0	36.0	36.0	36.0
20	35.091852546253726	36.0	36.0	36.0	36.0	36.0
21	35.08795099607092	36.0	36.0	36.0	36.0	36.0
22	35.068432157949125	36.0	36.0	36.0	36.0	36.0
23	35.034142100536506	36.0	36.0	36.0	36.0	36.0
24	35.01270976328047	36.0	36.0	36.0	36.0	36.0
25	34.986954561946334	36.0	36.0	36.0	36.0	36.0
26	34.94793523694319	36.0	36.0	36.0	32.0	36.0
27	34.92679193172421	36.0	36.0	36.0	32.0	36.0
28	34.91480971866402	36.0	36.0	36.0	32.0	36.0
29	34.887578007108814	36.0	36.0	36.0	32.0	36.0
30	34.865710210209635	36.0	36.0	36.0	32.0	36.0
31	34.8725533113093	36.0	36.0	36.0	32.0	36.0
32	34.83954822304556	36.0	36.0	36.0	32.0	36.0
33	34.82662665586492	36.0	36.0	36.0	32.0	36.0
34	34.81152549656354	36.0	36.0	36.0	32.0	36.0
35	34.78723418897266	36.0	36.0	36.0	32.0	36.0
36	34.756742456015324	36.0	36.0	36.0	32.0	36.0
37	34.74582499721864	36.0	36.0	36.0	32.0	36.0
38	34.71334139003765	36.0	36.0	36.0	32.0	36.0
39	34.690278850921786	36.0	36.0	36.0	32.0	36.0
40	34.69677786626304	36.0	36.0	36.0	32.0	36.0
41	34.664100806422326	36.0	36.0	36.0	32.0	36.0
42	34.64033633235932	36.0	36.0	36.0	32.0	36.0
43	34.635953062114744	36.0	36.0	36.0	32.0	36.0
44	34.599583962086335	36.0	36.0	36.0	32.0	36.0
45	34.557854770576995	36.0	36.0	36.0	32.0	36.0
46	34.54919986680058	36.0	36.0	36.0	32.0	36.0
47	34.52891830524765	36.0	36.0	36.0	32.0	36.0
48	34.49159876389103	36.0	36.0	36.0	32.0	36.0
49	34.47245383229337	36.0	36.0	36.0	32.0	36.0
50	33.96407706450499	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	20.0
21	64.0
22	174.0
23	553.0
24	1657.0
25	3812.0
26	7614.0
27	14487.0
28	24440.0
29	37873.0
30	56047.0
31	81271.0
32	120642.0
33	200410.0
34	445398.0
35	1621154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25820441581225	18.436983555607558	11.853376251033367	26.451435777546823
2	16.003662751575213	20.292752492812134	37.57455496421362	26.129029791399034
3	18.76331905242899	23.869844709502246	28.715131514053677	28.651704724015087
4	12.460649955307066	15.683090778268758	36.13716181135988	35.7190974550643
5	14.240409660857607	36.76277236777262	33.61767560894577	15.379142362424
6	34.34994729720895	35.53364777866433	16.56851878968241	13.547886134444303
7	30.172891690172833	30.502105423328867	20.669239409609784	18.655763476888517
8	27.652299047226535	34.017350333208825	19.32156228697746	19.008788332587176
9	27.355850050485024	14.29056971808289	18.920167133922384	39.4334130975097
10	16.19741652766239	26.734813488314657	31.590245856920728	25.477524127102217
11	37.36752220404515	20.98250247455008	22.72472336460818	18.925251956796593
12	24.858475615980414	23.62313127980404	28.895939673355564	22.62245343085998
13	29.70874669821041	19.59530162366423	25.365313938111207	25.330637740014154
14	23.011958509374615	20.331301060892855	25.114513651984783	31.542226777747747
15	24.75911129530319	28.467094123129943	22.03880752110297	24.734987060463897
16	25.16058291186014	25.95526808677231	24.680200961375608	24.20394803999194
17	23.701200515211077	26.4820633828906	25.525160888765868	24.291575213132454
18	24.419231029500764	24.903168532822146	27.357264625269583	23.320335812407503
19	25.546494205786985	25.013696524772072	25.456726054594174	23.983083214846765
20	25.661533544347108	24.70264299917381	25.483297121493244	24.15252633498584
21	26.15732289045801	24.446107950407303	24.697061163537462	24.69950799559723
22	25.65373426715659	25.235058362679386	25.0220692782266	24.08913809193742
23	23.80125300740511	24.99144564572854	25.971440117417355	25.235861229449004
24	24.725085036972015	25.248745329513728	25.796644552147534	24.229525081366724
25	24.463312238327557	25.131870866908773	25.85536852158202	24.54944837318165
26	23.680325979201164	26.070498584087105	26.38109332867416	23.868082108037576
27	24.66746978831462	25.498475126614	25.40970100094547	24.42435408412591
28	23.824994924735062	25.476835955585408	25.895206006055144	24.802963113624383
29	24.13903052690617	25.281892257573425	25.669715139046968	24.909362076473442
30	23.868540889048784	25.400143063211996	26.181485357048235	24.54983069069099
31	24.893992912598012	25.017443236363597	25.256162289194904	24.83240156184349
32	24.26993604210386	25.526651927052292	25.264611506151297	24.93880052469255
33	23.731824147709133	25.181686838375654	26.20522727437819	24.881261739537024
34	24.58137188521146	25.303951977862287	26.227439921670786	23.887236215255463
35	24.8709773985358	25.3247118186194	26.035401836729775	23.768908946115022
36	23.721845660715385	25.545576643764573	26.040563123105855	24.692014572414188
37	24.714418378461456	25.2979878247166	25.200305701080467	24.78728809574148
38	24.068110628923773	25.38855884267902	25.795841685377923	24.747488843019283
39	24.960554390973943	24.755058729704196	25.382556457782396	24.90183042153946
40	25.26075009930697	24.84043022953961	26.06594900572597	23.832870665427446
41	24.22325507421356	25.001194742216686	26.58934167601114	24.186208507558607
42	24.976458799362447	25.68011417530099	25.928008848356438	23.415418176980126
43	23.916942285731107	24.86226055932287	26.36014232916238	24.860654825783644
44	23.476321356217838	25.36768430666911	25.934202392007727	25.22179194510532
45	23.63008945847401	25.665586109946105	26.015291935738542	24.689032495841342
46	23.638795178511895	24.769576972837072	26.205563184922603	25.386064663728426
47	24.21197670768806	24.69247335342539	26.49100961260914	24.60454032627741
48	24.332139100873327	25.519234967371112	25.55494342274338	24.59368250901218
49	23.77250273070281	25.361681921772483	25.86649396110378	24.999321386420924
50	23.35048028673835	26.21847550776583	25.60512305854241	24.825921146953405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	58.0
2	61.0
3	234.5
4	408.0
5	442.5
6	477.0
7	427.5
8	378.0
9	525.0
10	672.0
11	1021.0
12	1370.0
13	2395.0
14	3420.0
15	4434.0
16	5448.0
17	6160.0
18	6872.0
19	7419.5
20	7967.0
21	9572.0
22	11177.0
23	12462.0
24	13747.0
25	16698.0
26	19649.0
27	25221.5
28	30794.0
29	36296.0
30	41798.0
31	47688.0
32	53578.0
33	63604.0
34	73630.0
35	85690.0
36	97750.0
37	108914.0
38	120078.0
39	122948.5
40	125819.0
41	132531.0
42	139243.0
43	139854.0
44	140465.0
45	151970.0
46	163475.0
47	172754.5
48	182034.0
49	187188.5
50	192343.0
51	185049.5
52	177756.0
53	173252.5
54	168749.0
55	169921.0
56	171093.0
57	166403.0
58	161713.0
59	148136.0
60	134559.0
61	117900.0
62	101241.0
63	88880.0
64	76519.0
65	65939.5
66	55360.0
67	48876.5
68	42393.0
69	38678.0
70	34963.0
71	27277.0
72	19591.0
73	17525.0
74	15459.0
75	11385.5
76	7312.0
77	6619.0
78	5926.0
79	5138.5
80	4351.0
81	3363.0
82	2375.0
83	2027.0
84	1679.0
85	1413.5
86	1148.0
87	832.0
88	516.0
89	321.5
90	127.0
91	87.5
92	48.0
93	32.0
94	16.0
95	14.5
96	13.0
97	11.5
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01487215111328947
2	0.004090797349927952
3	4.970127621407792E-4
4	3.8231750933906095E-5
5	0.0
6	6.117080149424975E-4
7	3.8231750933906095E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1469525280171828E-4
47	0.0
48	0.0
49	0.0
50	7.646350186781219E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2615627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.785784532114256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0626085364034	43.31507035822293
2	12.936137787045746	14.17432914957659
3	3.6328692063507995	5.970887687174634
4	1.407424418871758	3.08427403830177
5	0.7538022737031781	2.064882447346007
6	0.46606485851232815	1.532023734986803
7	0.296928890936973	1.1387237568162856
8	0.22810314604981186	0.9997447848465905
9	0.16145301210915128	0.7960796940125515
>10	0.8978099559221118	8.912126925290744
>50	0.07312943211099528	2.826004362113579
>100	0.0695614177954289	8.110817071198507
>500	0.009896000251397613	3.6615803839694094
>1k	0.004211063936760652	3.4134556061436983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2668	0.10200231149166147	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2649	0.10127590822391726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1469525280171829E-4
2	0.0	0.0	0.0	0.0	1.1469525280171829E-4
3	0.0	0.0	0.0	0.0	1.1469525280171829E-4
4	0.0	0.0	0.0	0.0	1.1469525280171829E-4
5	3.8231750933906095E-5	0.0	0.0	0.0	1.1469525280171829E-4
6	3.8231750933906095E-5	0.0	0.0	0.0	1.5292700373562438E-4
7	3.8231750933906095E-5	0.0	0.0	0.0	1.5292700373562438E-4
8	3.8231750933906095E-5	0.0	0.0	0.0	1.5292700373562438E-4
9	3.8231750933906095E-5	0.0	0.0	0.0	1.5292700373562438E-4
10	3.8231750933906095E-5	0.0	0.0	3.8231750933906095E-5	1.5292700373562438E-4
11	3.8231750933906095E-5	0.0	0.0	7.646350186781219E-5	1.5292700373562438E-4
12	3.8231750933906095E-5	0.0	0.0	7.646350186781219E-5	2.2939050560343658E-4
13	3.8231750933906095E-5	0.0	0.0	7.646350186781219E-5	2.6762225653734266E-4
14	3.8231750933906095E-5	0.0	0.0	1.5292700373562438E-4	2.6762225653734266E-4
15	3.8231750933906095E-5	0.0	0.0	1.5292700373562438E-4	3.8231750933906096E-4
16	3.8231750933906095E-5	0.0	0.0	1.9115875466953048E-4	3.8231750933906096E-4
17	3.8231750933906095E-5	0.0	0.0	1.9115875466953048E-4	3.8231750933906096E-4
18	3.8231750933906095E-5	0.0	0.0	1.9115875466953048E-4	3.8231750933906096E-4
19	3.8231750933906095E-5	0.0	0.0	2.2939050560343658E-4	3.8231750933906096E-4
20	3.8231750933906095E-5	0.0	0.0	3.8231750933906096E-4	4.2054926027296707E-4
21	3.8231750933906095E-5	0.0	0.0	4.970127621407792E-4	4.5878101120687317E-4
22	3.8231750933906095E-5	0.0	0.0	8.410985205459341E-4	4.5878101120687317E-4
23	3.8231750933906095E-5	0.0	0.0	0.0014145747845545256	4.970127621407792E-4
24	3.8231750933906095E-5	0.0	0.0	0.002714454316307333	4.970127621407792E-4
25	3.8231750933906095E-5	0.0	0.0	0.003632016338721079	4.970127621407792E-4
26	3.8231750933906095E-5	0.0	0.0	0.00451134661020092	4.970127621407792E-4
27	3.8231750933906095E-5	0.0	0.0	0.006728788164367473	5.352445130746853E-4
28	3.8231750933906095E-5	0.0	0.0	0.014183979596479162	5.352445130746853E-4
29	3.8231750933906095E-5	0.0	0.0	0.0307383277508605	5.352445130746853E-4
30	3.8231750933906095E-5	0.0	0.0	0.06239421752413475	5.352445130746853E-4
31	3.8231750933906095E-5	0.0	0.0	0.13014088017901634	5.352445130746853E-4
32	3.8231750933906095E-5	0.0	0.0	0.2095099951178054	5.734762640085914E-4
33	3.8231750933906095E-5	0.0	0.0	0.2933522249158615	5.734762640085914E-4
34	3.8231750933906095E-5	0.0	0.0	0.38736410046233655	6.117080149424975E-4
35	3.8231750933906095E-5	0.0	0.0	0.4916603170100324	6.117080149424975E-4
36	3.8231750933906095E-5	0.0	0.0	0.6399230471317202	6.117080149424975E-4
37	3.8231750933906095E-5	0.0	0.0	0.8532179855919824	6.117080149424975E-4
38	3.8231750933906095E-5	0.0	0.0	1.1332273294319106	6.499397658764036E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	2190	0.0	20.192019	2
GTCCTAC	2130	0.0	19.938698	1
GTATCAA	4780	0.0	19.565012	1
TCCTACA	2260	0.0	18.982523	2
GTAGGAC	2415	0.0	18.857363	3
TAGGACG	2040	0.0	18.225704	4
CTGTAGG	2340	0.0	17.961254	1
CGTCGTA	630	0.0	17.809393	10
TTAGGAC	1350	0.0	17.76317	3
TAGGACC	1945	0.0	17.758564	4
ACCGTCG	655	0.0	17.46552	8
AGGACGT	2095	0.0	17.432186	5
ATACCGT	695	0.0	17.410276	6
GACGTGA	1150	0.0	17.408566	7
CCGTCGT	670	0.0	17.074501	9
GGACGTG	2110	0.0	16.891193	6
CCTACAG	2350	0.0	16.757643	3
CTAGCGG	780	0.0	16.640903	29
GTATCGA	80	0.0019892817	16.49988	9
TACCGTC	695	0.0	16.46031	7
>>END_MODULE