FastQCFastQC Report
Thu 2 Feb 2017
SRR4062385_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062385_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865345
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG117841.3617690054255818No Hit
TCCATGTACTCTGCGTTGATACCAC101931.1779116999578203No Hit
GAGTACATGGAAGCAGTGGTATCAA50900.5882047044820273No Hit
CATGTACTCTGCGTTGATACCACTG42930.4961027104796353No Hit
GCTTCCATGTACTCTGCGTTGATAC34860.40284510802050044No Hit
CCCATGTACTCTGCGTTGATACCAC29170.33709098683184163No Hit
CATGGAAGCAGTGGTATCAACGCAG28010.3236859287336265No Hit
GCGTTGATACCACTGCTTCCATGTA26630.3077385320305774No Hit
GTACATGGGAAGCAGTGGTATCAAC25350.29294674378427105No Hit
GTATCAACGCAGAGTACATGGAAGC22720.2625542413719384No Hit
ACGCAGAGTACATGGAAGCAGTGGT21650.2501892308847916No Hit
GTATCAACGCAGAGTACTTTTTTTT20880.24129104576787294No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18590.21482761210846543No Hit
ACTCTGCGTTGATACCACTGCTTCC18030.20835620475070635No Hit
CAGTGGTATCAACGCAGAGTACATG16850.19472002496114266No Hit
TATCAACGCAGAGTACTTTTTTTTT15340.17727033726432812No Hit
TATCAACGCAGAGTACATGGAAGCA15170.17530580288786554No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12770.14757119992604106No Hit
GTATCAACGCAGAGTACATGGGAAG10820.12503683501955867No Hit
GGTATCAACGCAGAGTACATGGAAG10340.11948991442719378No Hit
ATACCACTGCTTCCATGTACTCTGC10070.11636977159398852No Hit
AAGCAGTGGTATCAACGCAGAGTAC9960.11509860229157157No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9330.10781826901409264No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9150.10573817379195581No Hit
GAGTACATGGGAAGCAGTGGTATCA8810.10180910503903068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1550.014.0898631
AGGCCCG1400.013.57262810
GCGATTT500.001497609113.30117616
TAAGCTG500.001500224913.2980965
GTTAGAC500.001501534413.2965573
CGTTATT500.001502189413.2957872
ATCGCCA1153.7471182E-1013.21855816
TCGCCAG1102.730303E-912.957190517
CGCATCG1102.73576E-912.9556913
AACAATA751.481602E-512.6626552
GCGTTAT604.1057082E-412.6604571
TTGGACT701.0918666E-412.211834
CGGAATT550.003064519512.09197715
ACAGGTA550.003064519512.0919778
ATAACGA550.003065848712.09127812
CAGTACT550.003071170812.0884784
TCTTATA550.003073834612.0870792
GCATCGC1209.9717E-911.8760514
CGGACCA1459.640644E-1111.7941469
ATACACT2100.011.759544
Produced by FastQC (version 0.11.5)