##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062385_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865345
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.117910197666827	32.0	32.0	32.0	32.0	32.0
2	30.76920881267009	32.0	32.0	32.0	32.0	32.0
3	30.756180482928773	32.0	32.0	32.0	32.0	32.0
4	30.82950037268373	32.0	32.0	32.0	32.0	32.0
5	30.59025128705892	32.0	32.0	32.0	32.0	32.0
6	34.23956918916733	36.0	36.0	36.0	32.0	36.0
7	34.11306242019079	36.0	36.0	36.0	32.0	36.0
8	34.10946501106495	36.0	36.0	36.0	32.0	36.0
9	34.28265489486852	36.0	36.0	36.0	32.0	36.0
10	33.925403162900345	36.0	36.0	36.0	32.0	36.0
11	34.29561504371089	36.0	36.0	36.0	32.0	36.0
12	34.05023545522306	36.0	36.0	36.0	32.0	36.0
13	34.16847153447469	36.0	36.0	36.0	32.0	36.0
14	34.05863903992049	36.0	36.0	36.0	32.0	36.0
15	33.99804933292502	36.0	36.0	36.0	32.0	36.0
16	33.985969757726686	36.0	36.0	36.0	32.0	36.0
17	33.89988270574164	36.0	36.0	36.0	32.0	36.0
18	33.920763394946526	36.0	36.0	36.0	32.0	36.0
19	33.91408975610884	36.0	36.0	36.0	32.0	36.0
20	33.92011163177692	36.0	36.0	36.0	32.0	36.0
21	33.862029595132576	36.0	36.0	36.0	32.0	36.0
22	33.80895134310593	36.0	36.0	36.0	32.0	36.0
23	33.70177212556842	36.0	36.0	36.0	27.0	36.0
24	33.70068007557679	36.0	36.0	36.0	27.0	36.0
25	33.163730072976676	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	33.0
5	86.0
6	141.0
7	27.0
8	129.0
9	152.0
10	120.0
11	37.0
12	53.0
13	54.0
14	139.0
15	197.0
16	300.0
17	395.0
18	566.0
19	815.0
20	1214.0
21	1809.0
22	2743.0
23	4025.0
24	5551.0
25	7882.0
26	10950.0
27	13839.0
28	19079.0
29	25762.0
30	33522.0
31	45838.0
32	66022.0
33	93156.0
34	197735.0
35	332972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.32492560168732	16.897980410852043	11.613995550547514	25.163098436913124
2	15.198139885337614	20.58098536615807	37.31439011790973	26.906484630594584
3	19.945661155353392	24.88677481275518	27.74320446637105	27.424359565520373
4	12.32003069750132	15.71143424135217	35.395166761633206	36.573368299513305
5	13.610096075482428	37.445740067903664	34.0095185359442	14.934645320669695
6	34.28578365292348	34.19317842864773	17.54204230937313	13.978995609055659
7	29.17852889140716	30.730928896032562	21.48903201933417	18.6015101932261
8	28.49666263013198	32.89625411894245	20.390977130544307	18.216106120381262
9	26.96011751914313	15.060841603627361	18.388044509218776	39.590996368010735
10	15.250741012404582	27.716640558657407	32.606165500868855	24.426452928069157
11	36.90436798737525	21.493543005365982	21.042097152258272	20.559991855000497
12	23.18568239910301	24.236337149092307	29.737926437967978	22.840054013836706
13	30.639925027767497	19.785033320992227	24.986231951129213	24.58880970011107
14	23.921026872800226	19.5140773857044	25.047495412440675	31.517400329054695
15	25.821446337879895	26.35141544904499	24.37210055646178	23.455037656613335
16	25.9807959278112	25.483456732993986	25.255090236001855	23.28065710319297
17	23.773954272980657	25.766195419893258	26.967741636902797	23.49210867022329
18	25.389236522511847	23.81840565758294	28.18215898992891	22.6101988299763
19	24.31996797126671	27.62223682748917	25.255257896101014	22.80253730514311
20	24.17610260812623	23.868583665861813	28.40755399803549	23.547759727976473
21	25.21873951427216	25.891793073923658	23.761093176900737	25.128374234903443
22	23.948449277324695	26.122088091278478	25.176922070963254	24.75254056043357
23	22.72943447184162	25.504136190539008	25.191014590355714	26.575414747263657
24	23.164951841412222	27.250563508703724	25.098353675187568	24.486130974696493
25	25.13442306557485	24.665808117799383	24.509834549996587	25.689934266629177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	149.0
1	149.0
2	378.0
3	607.0
4	607.0
5	607.0
6	1652.5
7	2698.0
8	2698.0
9	2698.0
10	2723.0
11	2748.0
12	2748.0
13	2748.0
14	2657.5
15	2567.0
16	2567.0
17	2567.0
18	4234.0
19	5901.0
20	5901.0
21	5901.0
22	9407.5
23	12914.0
24	12914.0
25	12914.0
26	19095.0
27	25276.0
28	25276.0
29	25276.0
30	31939.5
31	38603.0
32	38603.0
33	38603.0
34	45497.5
35	52392.0
36	52392.0
37	52392.0
38	59474.0
39	66556.0
40	66556.0
41	66556.0
42	79637.5
43	92719.0
44	92719.0
45	92719.0
46	124067.5
47	155416.0
48	155416.0
49	155416.0
50	137166.0
51	118916.0
52	118916.0
53	118916.0
54	103403.5
55	87891.0
56	87891.0
57	87891.0
58	79833.0
59	71775.0
60	71775.0
61	71775.0
62	63000.5
63	54226.0
64	54226.0
65	54226.0
66	45009.5
67	35793.0
68	35793.0
69	35793.0
70	26907.0
71	18021.0
72	18021.0
73	18021.0
74	13896.0
75	9771.0
76	9771.0
77	9771.0
78	7974.0
79	6177.0
80	6177.0
81	6177.0
82	4210.5
83	2244.0
84	2244.0
85	2244.0
86	1665.0
87	1086.0
88	1086.0
89	1086.0
90	737.5
91	389.0
92	389.0
93	389.0
94	238.0
95	87.0
96	87.0
97	87.0
98	255.0
99	423.0
100	423.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008089259197198806
2	0.0025423386048339103
3	0.003929068752925134
4	0.014791788246306387
5	0.034206010319583514
6	0.04425980389324489
7	0.0641362693492191
8	0.08574614749030733
9	0.09302648076778626
10	0.11405855468050316
11	0.1168320149766856
12	0.12965926884652942
13	0.11844986681612535
14	0.12145444880365636
15	0.11036060761892656
16	0.10920499916218387
17	0.12041440119258792
18	0.12584576093927854
19	0.12954370800085513
20	0.1164853324396628
21	0.12422790909983879
22	0.14606890893227556
23	0.1322016074513633
24	0.1290814646181581
25	0.12665468685899844
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	865345.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.3676075196996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91812462447604	42.374885915647006
2	12.095891107244798	12.668657562104402
3	3.23581216602898	5.083552245542239
4	1.2054310250816345	2.5250215525417676
5	0.6397609597716799	1.675137542387483
6	0.37162426342218596	1.1676644143014292
7	0.24472017268895221	0.897078696886971
8	0.17762328477810702	0.7441365170895792
9	0.12600037379931406	0.5938504310212127
>10	0.7755842488972893	7.884517745409991
>50	0.10327693894834758	3.8204753582801927
>100	0.09155509507218165	9.44497531131881
>500	0.009951640766279366	3.582572848318602
>1k	0.0039806563065117465	4.402852902776035
>5k	2.2114757258398594E-4	0.5894713366071338
>10k+	4.422951451679719E-4	2.545149619767187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11784	1.3617690054255818	No Hit
TCCATGTACTCTGCGTTGATACCAC	10193	1.1779116999578203	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5090	0.5882047044820273	No Hit
CATGTACTCTGCGTTGATACCACTG	4293	0.4961027104796353	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3486	0.40284510802050044	No Hit
CCCATGTACTCTGCGTTGATACCAC	2917	0.33709098683184163	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2801	0.3236859287336265	No Hit
GCGTTGATACCACTGCTTCCATGTA	2663	0.3077385320305774	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2535	0.29294674378427105	No Hit
GTATCAACGCAGAGTACATGGAAGC	2272	0.2625542413719384	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2165	0.2501892308847916	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2088	0.24129104576787294	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1859	0.21482761210846543	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1803	0.20835620475070635	No Hit
CAGTGGTATCAACGCAGAGTACATG	1685	0.19472002496114266	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1534	0.17727033726432812	No Hit
TATCAACGCAGAGTACATGGAAGCA	1517	0.17530580288786554	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1277	0.14757119992604106	No Hit
GTATCAACGCAGAGTACATGGGAAG	1082	0.12503683501955867	No Hit
GGTATCAACGCAGAGTACATGGAAG	1034	0.11948991442719378	No Hit
ATACCACTGCTTCCATGTACTCTGC	1007	0.11636977159398852	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	996	0.11509860229157157	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	933	0.10781826901409264	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	915	0.10573817379195581	No Hit
GAGTACATGGGAAGCAGTGGTATCA	881	0.10180910503903068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.311216913485373E-4
2	0.0	0.0	0.0	0.0	2.311216913485373E-4
3	0.0	0.0	0.0	0.0	2.311216913485373E-4
4	0.0	0.0	0.0	0.0	2.311216913485373E-4
5	0.0	0.0	0.0	0.0	2.311216913485373E-4
6	0.0	0.0	0.0	0.0	2.311216913485373E-4
7	0.0	0.0	0.0	0.0	2.311216913485373E-4
8	0.0	0.0	0.0	0.0	2.311216913485373E-4
9	0.0	0.0	0.0	0.0	2.311216913485373E-4
10	0.0	0.0	0.0	0.0	2.311216913485373E-4
11	0.0	0.0	0.0	0.0	2.311216913485373E-4
12	0.0	0.0	0.0	0.0	5.778042283713432E-4
13	0.0	0.0	0.0	1.1556084567426865E-4	6.933650740456119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	155	0.0	14.089863	1
AGGCCCG	140	0.0	13.572628	10
GCGATTT	50	0.0014976091	13.301176	16
TAAGCTG	50	0.0015002249	13.298096	5
GTTAGAC	50	0.0015015344	13.296557	3
CGTTATT	50	0.0015021894	13.295787	2
ATCGCCA	115	3.7471182E-10	13.218558	16
TCGCCAG	110	2.730303E-9	12.9571905	17
CGCATCG	110	2.73576E-9	12.95569	13
AACAATA	75	1.481602E-5	12.662655	2
GCGTTAT	60	4.1057082E-4	12.660457	1
TTGGACT	70	1.0918666E-4	12.21183	4
CGGAATT	55	0.0030645195	12.091977	15
ACAGGTA	55	0.0030645195	12.091977	8
ATAACGA	55	0.0030658487	12.091278	12
CAGTACT	55	0.0030711708	12.088478	4
TCTTATA	55	0.0030738346	12.087079	2
GCATCGC	120	9.9717E-9	11.87605	14
CGGACCA	145	9.640644E-11	11.794146	9
ATACACT	210	0.0	11.75954	4
>>END_MODULE