FastQCFastQC Report
Thu 2 Feb 2017
SRR4062385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865345
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA69270.8004899779856588No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC53970.6236818841040279No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC24410.28208402429088975No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA23870.27584373862447925No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG18170.20997405659014612No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG15520.17935043248646493No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA14840.17149229498061466No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC13910.16074513633290768No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11560.13358833759945454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10990.12700136939602125No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10910.12607688263062708No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9480.10955168169920668No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8950.10342695687847045No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG8820.10192466588470495No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8680.10030681404526519No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC8660.10007569235391664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA454.068589E-529.3329474
TATAACG500.002577451522.0035252
TAACGGC703.2143173E-521.99971236
GGCGTTA1551.8189894E-1221.29004342
AGTACTC3000.020.5330645
TCGTATA550.0044829419.99973944
TCATAGG1001.1228221E-619.799743
GTAGGAC3450.019.7678573
GACCGAG1452.9467628E-1019.7238797
ATAACGC2350.019.6593173
TTAACGG901.0015341E-519.55529835
GCATAAG708.099429E-418.8645251
CGCGTAT708.121492E-418.85689542
GCGGCGT1801.4551915E-1118.33309240
GCGTAAC1203.159821E-718.33309211
TGGTATA2400.018.33309244
GTGTTAC851.4259557E-418.124741
GTCATAA851.4259557E-418.124741
TACGACG2550.018.1174095
GTCCTAA1951.8189894E-1218.058351
Produced by FastQC (version 0.11.5)