##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865345
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231563133779012	32.0	32.0	32.0	32.0	32.0
2	31.383633117427152	32.0	32.0	32.0	32.0	32.0
3	31.496162802119386	32.0	32.0	32.0	32.0	32.0
4	31.60934540558968	32.0	32.0	32.0	32.0	32.0
5	31.541321669391976	32.0	32.0	32.0	32.0	32.0
6	35.14318682144116	36.0	36.0	36.0	36.0	36.0
7	35.15601291970255	36.0	36.0	36.0	36.0	36.0
8	35.1051465022621	36.0	36.0	36.0	36.0	36.0
9	35.23147415192784	36.0	36.0	36.0	36.0	36.0
10	35.10731904616078	36.0	36.0	36.0	36.0	36.0
11	35.24072017519024	36.0	36.0	36.0	36.0	36.0
12	35.14064448283632	36.0	36.0	36.0	36.0	36.0
13	35.176247623780114	36.0	36.0	36.0	36.0	36.0
14	35.140751954422804	36.0	36.0	36.0	36.0	36.0
15	35.10138730795232	36.0	36.0	36.0	36.0	36.0
16	35.10327903899601	36.0	36.0	36.0	36.0	36.0
17	35.08273347624358	36.0	36.0	36.0	36.0	36.0
18	35.09088282708053	36.0	36.0	36.0	36.0	36.0
19	35.06046952371597	36.0	36.0	36.0	36.0	36.0
20	35.060742247311765	36.0	36.0	36.0	36.0	36.0
21	35.02194038215972	36.0	36.0	36.0	36.0	36.0
22	34.989236662833896	36.0	36.0	36.0	36.0	36.0
23	34.91568102895377	36.0	36.0	36.0	32.0	36.0
24	34.904362999728434	36.0	36.0	36.0	32.0	36.0
25	34.859082793567886	36.0	36.0	36.0	32.0	36.0
26	34.80491711398344	36.0	36.0	36.0	32.0	36.0
27	34.77353078829831	36.0	36.0	36.0	32.0	36.0
28	34.72715968775459	36.0	36.0	36.0	32.0	36.0
29	34.69850637606966	36.0	36.0	36.0	32.0	36.0
30	34.67516424085191	36.0	36.0	36.0	32.0	36.0
31	34.64858986878066	36.0	36.0	36.0	32.0	36.0
32	34.6035477179622	36.0	36.0	36.0	32.0	36.0
33	34.56583328036795	36.0	36.0	36.0	32.0	36.0
34	34.531495530684296	36.0	36.0	36.0	32.0	36.0
35	34.454850955399294	36.0	36.0	36.0	32.0	36.0
36	34.410152020292486	36.0	36.0	36.0	32.0	36.0
37	34.3530060264981	36.0	36.0	36.0	32.0	36.0
38	34.26600026578995	36.0	36.0	36.0	32.0	36.0
39	34.12938192281691	36.0	36.0	36.0	32.0	36.0
40	34.023186128076084	36.0	36.0	36.0	32.0	36.0
41	34.03927450901086	36.0	36.0	36.0	32.0	36.0
42	33.88020500494023	36.0	36.0	36.0	32.0	36.0
43	33.911267760257466	36.0	36.0	36.0	32.0	36.0
44	33.89610155487118	36.0	36.0	36.0	32.0	36.0
45	33.697960928878075	36.0	36.0	36.0	27.0	36.0
46	33.80679035529182	36.0	36.0	36.0	32.0	36.0
47	33.71467680520486	36.0	36.0	36.0	27.0	36.0
48	33.72741276600662	36.0	36.0	36.0	27.0	36.0
49	33.77473377670178	36.0	36.0	36.0	32.0	36.0
50	33.13996729628067	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	12.0
22	84.0
23	207.0
24	696.0
25	1699.0
26	3501.0
27	6594.0
28	11507.0
29	17576.0
30	25439.0
31	36081.0
32	53886.0
33	76372.0
34	151538.0
35	480141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.17629327582102	17.309981669105394	12.136069329153981	25.377655725919606
2	14.811360657897474	21.08211930632311	37.03342879133528	27.073091244444136
3	19.118887648525778	25.236381621978925	28.250232856987672	27.39449787250762
4	12.174219530938528	16.29951059981857	35.5318399020044	35.9944299672385
5	13.527783716321236	37.541327447434256	33.918841618082965	15.012047218161543
6	34.458171884082425	34.44083763704167	17.261443203183955	13.839547275691954
7	29.302185833395928	30.757905806354692	21.370551629696827	18.569356730552556
8	28.56860558505567	33.01746702182366	20.293062304629945	18.120865088490717
9	27.214579156290263	14.80542442609595	18.201988802154055	39.77800761545973
10	15.288815443551416	27.75182152782994	32.58446053308218	24.374902495536464
11	37.286515782722496	21.354604232993776	21.012890812334962	20.345989171948762
12	23.322143191443875	24.19636098896972	29.623907227753094	22.857588591833313
13	30.978395899901194	19.743223800911775	24.828478814807966	24.449901484379062
14	24.029722249507422	19.441956676238956	24.98876170775818	31.539559366495446
15	25.827617886507692	26.586968203433315	24.222015496709403	23.36339841334959
16	26.060704112232692	25.49757611126198	25.17134784392352	23.270371932581803
17	23.824486187589923	25.792371828577043	26.93295737538207	23.450184608450964
18	25.574308512789695	23.875217398840924	28.13617690054256	22.414297187826822
19	24.61827363652647	27.630482639871957	25.035563850256253	22.715679873345312
20	24.368662209870052	23.762314452617165	28.291721798820124	23.57730153869266
21	25.401545048506662	25.75793469656611	23.769941468431664	25.070578786495563
22	23.976679821342934	26.1150177096996	25.07208107748933	24.836221391468143
23	23.078540928762518	25.265067689765353	25.05971606700218	26.59667531446995
24	23.37842132328724	27.124903940047034	25.09091749533423	24.40575724133149
25	25.41275445053707	24.45683513511952	24.397667982134294	25.732742432209122
26	22.947957173150595	25.313141001565846	26.038285308171886	25.700616517111673
27	25.597073999387526	25.767757368448425	25.397153736371042	23.238014895793007
28	24.25217687743039	24.134767058225332	25.70211880810544	25.910937256238842
29	23.13793920343909	26.11293761447746	26.876332561001682	23.872790621081766
30	25.322501430065465	24.824665306900716	26.43720134743946	23.41563191559436
31	23.449144560839898	25.25824959987057	25.564485840907388	25.728119998382148
32	23.530614957040257	25.931391525923186	25.6645615332613	24.87343198377526
33	23.059588950071934	25.129168135252417	26.112013127712068	25.699229786963578
34	23.039250241233265	26.44367275479722	27.113001172942585	23.404075831026933
35	25.52831529621134	25.2186122298043	25.98535844085307	23.267714033131295
36	23.82587291773801	26.344983792591393	25.8495744471858	23.979568842484788
37	25.434248767832486	25.848187717037714	25.746147490307337	22.97141602482247
38	24.031917905575234	25.726039903160014	24.83344793117196	25.408594260092794
39	24.04474515944508	25.402816217809082	26.194523571523497	24.357915051222346
40	25.59256712640623	25.4907580213672	24.68402775771513	24.232647094511435
41	22.88185636942491	25.646534041336118	26.653646811387365	24.81796277785161
42	26.396755051453468	25.805199082446883	25.461636688257283	22.336409177842363
43	24.894579618533648	23.992396096354632	25.344342429897903	25.768681855213813
44	24.262692914386747	25.030132490509565	26.915623248530935	23.791551346572753
45	24.91630505752041	25.254436091963324	25.798958796780475	24.030300053735793
46	23.593187903524964	24.89856623327397	27.029166469249766	24.4790793939513
47	25.13078598709185	24.177524571124813	27.298476330249784	23.39321311153355
48	24.10495236004137	27.876627241158154	24.545008060368986	23.473412338431494
49	23.256620191946563	25.250853705747417	27.80359278669201	23.688933315614
50	23.30388457782734	27.85883087092431	23.98234230278097	24.854942248467374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	22.0
2	28.0
3	119.5
4	211.0
5	229.5
6	248.0
7	210.0
8	172.0
9	247.5
10	323.0
11	469.5
12	616.0
13	1005.0
14	1394.0
15	1812.0
16	2230.0
17	2563.0
18	2896.0
19	3154.0
20	3412.0
21	4104.5
22	4797.0
23	5207.0
24	5617.0
25	6086.5
26	6556.0
27	8152.0
28	9748.0
29	11083.5
30	12419.0
31	13828.0
32	15237.0
33	17349.0
34	19461.0
35	22374.5
36	25288.0
37	28854.5
38	32421.0
39	34295.0
40	36169.0
41	39534.5
42	42900.0
43	45481.5
44	48063.0
45	59028.0
46	69993.0
47	78701.5
48	87410.0
49	82591.5
50	77773.0
51	68323.0
52	58873.0
53	55385.0
54	51897.0
55	52236.0
56	52575.0
57	50309.5
58	48044.0
59	44718.5
60	41393.0
61	36400.0
62	31407.0
63	27084.5
64	22762.0
65	19381.0
66	16000.0
67	14027.5
68	12055.0
69	11064.0
70	10073.0
71	7699.0
72	5325.0
73	4712.5
74	4100.0
75	2865.5
76	1631.0
77	1511.5
78	1392.0
79	1192.0
80	992.0
81	769.0
82	546.0
83	491.0
84	436.0
85	348.0
86	260.0
87	189.5
88	119.0
89	78.0
90	37.0
91	27.0
92	17.0
93	10.5
94	4.0
95	3.5
96	3.0
97	4.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01606295754872334
2	0.004969116363993551
3	8.089259197198805E-4
4	0.0
5	0.0
6	6.933650740456119E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.311216913485373E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	865345.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.28651209679042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42753128748826	46.64699256112477
2	11.817550988304145	13.53972555291846
3	3.1629057824554447	5.435755211029266
4	1.1981233438105932	2.7454522971461013
5	0.6044200156398722	1.73125572687479
6	0.3469208308533239	1.1924330623984514
7	0.233479927897145	0.9362675499686286
8	0.17499675257791858	0.8019962866763177
9	0.11566751797965559	0.5963569801152537
>10	0.7395456061308301	8.151759770299307
>50	0.09155214389874552	3.7086685363817082
>100	0.0786156420349952	8.893657370609796
>500	0.006467096505425775	2.523645754037452
>1k	0.0018188708921509991	1.6692322452386454
>5k	4.0419353158911096E-4	1.4268010951810974
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6927	0.8004899779856588	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5397	0.6236818841040279	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2441	0.28208402429088975	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2387	0.27584373862447925	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1817	0.20997405659014612	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1552	0.17935043248646493	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1484	0.17149229498061466	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1391	0.16074513633290768	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1156	0.13358833759945454	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1099	0.12700136939602125	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1091	0.12607688263062708	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	948	0.10955168169920668	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	895	0.10342695687847045	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	882	0.10192466588470495	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	868	0.10030681404526519	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	866	0.10007569235391664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1556084567426865E-4
7	1.1556084567426865E-4	0.0	0.0	0.0	1.1556084567426865E-4
8	1.1556084567426865E-4	0.0	0.0	0.0	1.1556084567426865E-4
9	1.1556084567426865E-4	0.0	0.0	0.0	1.1556084567426865E-4
10	1.1556084567426865E-4	0.0	0.0	0.0	1.1556084567426865E-4
11	1.1556084567426865E-4	0.0	0.0	0.0	1.1556084567426865E-4
12	1.1556084567426865E-4	0.0	0.0	0.0	5.778042283713432E-4
13	1.1556084567426865E-4	0.0	0.0	1.1556084567426865E-4	5.778042283713432E-4
14	1.1556084567426865E-4	0.0	0.0	1.1556084567426865E-4	5.778042283713432E-4
15	2.311216913485373E-4	0.0	0.0	1.1556084567426865E-4	9.244867653941492E-4
16	3.4668253702280596E-4	0.0	0.0	3.4668253702280596E-4	0.0010400476110684178
17	4.622433826970746E-4	0.0	0.0	3.4668253702280596E-4	0.0010400476110684178
18	4.622433826970746E-4	0.0	0.0	5.778042283713432E-4	0.0010400476110684178
19	4.622433826970746E-4	0.0	0.0	9.244867653941492E-4	0.0010400476110684178
20	4.622433826970746E-4	0.0	0.0	0.0013867301480912238	0.0011556084567426864
21	4.622433826970746E-4	0.0	0.0	0.0018489735307882984	0.0015022909937654923
22	4.622433826970746E-4	0.0	0.0	0.003235703678879522	0.0015022909937654923
23	5.778042283713432E-4	0.0	0.0	0.004737994672645014	0.0015022909937654923
24	6.933650740456119E-4	0.0	0.0	0.007973698351524536	0.0015022909937654923
25	6.933650740456119E-4	0.0	0.0	0.009822671882312835	0.0015022909937654923
26	6.933650740456119E-4	0.0	0.0	0.012827253869843819	0.001617851839439761
27	6.933650740456119E-4	0.0	0.0	0.01617851839439761	0.001617851839439761
28	9.244867653941492E-4	0.0	0.0	0.02368997336322507	0.001617851839439761
29	9.244867653941492E-4	0.0	0.0	0.032934841017166566	0.001617851839439761
30	9.244867653941492E-4	0.0	0.0	0.05038452871398113	0.001617851839439761
31	9.244867653941492E-4	0.0	0.0	0.07950586182389682	0.001617851839439761
32	9.244867653941492E-4	0.0	0.0	0.1168320149766856	0.0017334126851140297
33	9.244867653941492E-4	0.0	0.0	0.15438928982082292	0.0017334126851140297
34	9.244867653941492E-4	0.0	0.0	0.20720059629396367	0.0017334126851140297
35	0.0010400476110684178	0.0	0.0	0.2581629292363162	0.0017334126851140297
36	0.0010400476110684178	0.0	0.0	0.3390555212083042	0.0017334126851140297
37	0.0010400476110684178	0.0	0.0	0.4580831922528009	0.0017334126851140297
38	0.0011556084567426864	0.0	0.0	0.5980273763643402	0.0017334126851140297
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	45	4.068589E-5	29.332947	4
TATAACG	50	0.0025774515	22.003525	2
TAACGGC	70	3.2143173E-5	21.999712	36
GGCGTTA	155	1.8189894E-12	21.290043	42
AGTACTC	300	0.0	20.533064	5
TCGTATA	55	0.00448294	19.999739	44
TCATAGG	100	1.1228221E-6	19.79974	3
GTAGGAC	345	0.0	19.767857	3
GACCGAG	145	2.9467628E-10	19.723879	7
ATAACGC	235	0.0	19.659317	3
TTAACGG	90	1.0015341E-5	19.555298	35
GCATAAG	70	8.099429E-4	18.864525	1
CGCGTAT	70	8.121492E-4	18.856895	42
GCGGCGT	180	1.4551915E-11	18.333092	40
GCGTAAC	120	3.159821E-7	18.333092	11
TGGTATA	240	0.0	18.333092	44
GTGTTAC	85	1.4259557E-4	18.12474	1
GTCATAA	85	1.4259557E-4	18.12474	1
TACGACG	255	0.0	18.117409	5
GTCCTAA	195	1.8189894E-12	18.05835	1
>>END_MODULE