FastQCFastQC Report
Thu 2 Feb 2017
SRR4062384_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062384_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534533
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25560.1665653329058417No Hit
GCGCAAGACGGACCAGAGCGAAAGC17920.11677819896997979No Hit
TATCAACGCAGAGTACTTTTTTTTT17060.11117388808191157No Hit
GAATAGGACCGCGGTTCTATTTTGT15800.10296292096683486No Hit
GAACTACGACGGTATCTGATCGTCT15550.10133376082495456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT350.002172273416.2833655
ATAAGGG456.781382E-414.7722723
CAAGACG3050.014.0134574
AAGACGG3350.013.0428945
GTAAGAC604.1048526E-412.6619473
GATAACG1650.012.09232111
AACGGAC1353.7107384E-1011.96279915
GTAAACG1209.973519E-911.87793717
CGAGCCG3050.011.83590215
CTACGCA658.037422E-411.6898584
CGGTTCT4150.011.67606312
CCCAATT1552.5465852E-1111.64538113
ACGAACG1552.5465852E-1111.64515
CGACGGG1800.011.61095214
GTTCTAA907.5021544E-611.6052711
GGTTCTA4250.011.40021613
ATAATAC1251.841363E-811.3957543
GTATTAT752.0834924E-411.3942661
ACTCTAA2600.011.32824610
GCGCAAG3950.011.2981111
Produced by FastQC (version 0.11.5)