FastQCFastQC Report
Thu 2 Feb 2017
SRR4062384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534533
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16920.1102615584024586No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16710.10889306388327914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG602.8734966E-421.999745
GTTATAT2650.020.760561
ATCGTTT4150.019.0841129
GTATCAA24500.018.682811
GTACTAA1254.840276E-717.6049541
CACGACT750.001291188317.5997944
CCGTTAT750.001291188317.599799
GTCCTAA2900.017.4531881
ATTTCGT1154.2748816E-617.21718842
ACCGTCG3850.017.1426548
TATATCA3100.017.0320574
CGTTTAT4800.016.95813231
TGGTCGG5300.016.60357737
CATCGTT4650.016.55894328
CGCAATA4550.016.43936536
TAGGACG2950.016.4065864
ATACCGT3700.016.0538656
GCTATAT1105.6093064E-516.0045051
CCCTATA1653.101377E-816.0045031
ATAACGC5500.016.0003323
Produced by FastQC (version 0.11.5)