##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062384_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1534533
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244974203878314	32.0	32.0	32.0	32.0	32.0
2	31.262262199639892	32.0	32.0	32.0	32.0	32.0
3	31.39604492050676	32.0	32.0	32.0	32.0	32.0
4	31.530963491824547	32.0	32.0	32.0	32.0	32.0
5	31.428989145231807	32.0	32.0	32.0	32.0	32.0
6	35.012522376514546	36.0	36.0	36.0	36.0	36.0
7	35.02393431747639	36.0	36.0	36.0	36.0	36.0
8	34.95766659954527	36.0	36.0	36.0	36.0	36.0
9	35.120153818783955	36.0	36.0	36.0	36.0	36.0
10	34.959647658277795	36.0	36.0	36.0	32.0	36.0
11	35.129927476307124	36.0	36.0	36.0	36.0	36.0
12	35.018556785680076	36.0	36.0	36.0	36.0	36.0
13	35.08115628663574	36.0	36.0	36.0	36.0	36.0
14	35.0335235540715	36.0	36.0	36.0	36.0	36.0
15	34.9792060516131	36.0	36.0	36.0	32.0	36.0
16	35.004623556482656	36.0	36.0	36.0	36.0	36.0
17	34.97275457745125	36.0	36.0	36.0	32.0	36.0
18	34.989677641341046	36.0	36.0	36.0	36.0	36.0
19	34.973160564158604	36.0	36.0	36.0	32.0	36.0
20	34.96968132975961	36.0	36.0	36.0	32.0	36.0
21	34.96173233159534	36.0	36.0	36.0	32.0	36.0
22	34.939807746069974	36.0	36.0	36.0	32.0	36.0
23	34.90055476161152	36.0	36.0	36.0	32.0	36.0
24	34.87895405312235	36.0	36.0	36.0	32.0	36.0
25	34.85402399296724	36.0	36.0	36.0	32.0	36.0
26	34.81426857552102	36.0	36.0	36.0	32.0	36.0
27	34.79177313228194	36.0	36.0	36.0	32.0	36.0
28	34.78310860698336	36.0	36.0	36.0	32.0	36.0
29	34.74675878589773	36.0	36.0	36.0	32.0	36.0
30	34.74447144505853	36.0	36.0	36.0	32.0	36.0
31	34.74673858431197	36.0	36.0	36.0	32.0	36.0
32	34.711005237424025	36.0	36.0	36.0	32.0	36.0
33	34.692930683145946	36.0	36.0	36.0	32.0	36.0
34	34.68030143372609	36.0	36.0	36.0	32.0	36.0
35	34.66524864567917	36.0	36.0	36.0	32.0	36.0
36	34.63124416353379	36.0	36.0	36.0	32.0	36.0
37	34.63293523176106	36.0	36.0	36.0	32.0	36.0
38	34.59687540118068	36.0	36.0	36.0	32.0	36.0
39	34.58875892535384	36.0	36.0	36.0	32.0	36.0
40	34.58569545262304	36.0	36.0	36.0	32.0	36.0
41	34.550606601487225	36.0	36.0	36.0	32.0	36.0
42	34.53878671882586	36.0	36.0	36.0	32.0	36.0
43	34.5266592507297	36.0	36.0	36.0	32.0	36.0
44	34.48937755004291	36.0	36.0	36.0	32.0	36.0
45	34.45474942539522	36.0	36.0	36.0	32.0	36.0
46	34.461645986107825	36.0	36.0	36.0	32.0	36.0
47	34.45185017200673	36.0	36.0	36.0	32.0	36.0
48	34.411030587155835	36.0	36.0	36.0	32.0	36.0
49	34.402889348094824	36.0	36.0	36.0	32.0	36.0
50	33.83504558064245	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	34.0
22	85.0
23	335.0
24	910.0
25	2065.0
26	4557.0
27	8405.0
28	14723.0
29	23843.0
30	35872.0
31	53944.0
32	82158.0
33	139011.0
34	304596.0
35	863988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09583180715541	18.529928946370216	11.694194354032433	26.680044892441934
2	16.420298326937075	20.63244424372642	36.43025602626539	26.517001403071117
3	18.402147378792876	23.80617547353508	28.970063641967986	28.821613505704057
4	12.531964142813573	16.0557094931875	36.26851704623951	35.14380931775942
5	14.539732935036263	36.46523079008402	33.552096957185015	15.442939317694698
6	34.06161515778498	35.90348805004806	16.618693080920806	13.416203711246153
7	30.144259060260104	30.642326547981106	20.791629494614316	18.42178489714447
8	27.832376364665993	33.045493319465926	19.625188901118452	19.49694141474963
9	27.444245252464434	14.188029843607142	18.797901381071636	39.56982352285679
10	15.934880514136873	26.560197793074508	31.577750364443126	25.927171328345494
11	37.452697335280504	21.1777133499247	22.349209824747984	19.02037949004681
12	24.450370242933843	24.058654978420144	28.561132279331886	22.929842499314123
13	29.205171866620006	19.493226929626147	25.448719577878094	25.852881625875757
14	23.264471992456336	20.044469555232762	24.916635875539985	31.77442257677091
15	25.1595110694915	27.481064271670924	21.990599094317297	25.368825564520282
16	25.936815956385427	25.945874086774285	23.546251530595956	24.571058426244335
17	24.06523678539334	25.940269775886215	25.22428647673266	24.770206961987785
18	25.06795226951783	24.988514420999746	26.021010952517802	23.922522356964627
19	25.642263802733474	25.341325341325344	25.06026263364815	23.956148222293034
20	25.48273644164055	24.803441828882143	24.882162846937796	24.831658882539507
21	26.684926293536858	24.51859946967579	24.145456630779528	24.651017606007823
22	25.63203267704246	24.884834669570484	24.649583945082966	24.83354870830409
23	24.400517942592305	24.559458806034147	25.29590435656972	25.744118894803826
24	24.63387884131524	25.492772068114533	24.90340709518792	24.969941995382307
25	24.813738120978826	24.835764366097045	25.19163810748938	25.158859405434747
26	24.429060828278047	25.530959581840207	25.622909380247933	24.41707020963381
27	25.343150000684243	25.211709360437347	24.658055577820743	24.787085061057663
28	24.349362314137267	25.03067708547161	25.610462596763966	25.00949800362716
29	24.528439596932746	25.276875766112557	25.225720137657515	24.96896449929718
30	24.604358459544372	25.26332115373211	25.585829695418738	24.546490691304783
31	24.945634926065456	25.032697244047537	24.64287180529842	25.37879602458859
32	24.664832884010966	25.206756713606026	24.735603600574247	25.39280680180876
33	24.463012525634834	24.884052672702378	25.179386823222437	25.473547978440347
34	24.851208804242074	25.058242475072223	25.660054231482803	24.430494489202903
35	25.764581146185844	24.768577801845904	25.119433730001244	24.34740732196701
36	24.40253810116824	25.350839636553925	25.11624057612316	25.130381686154678
37	25.554940819128685	25.102229798902986	24.57373024887702	24.769099133091306
38	24.59126001200365	25.034978068246172	25.28606422931276	25.087697690437416
39	25.013929319213073	24.96694434072125	24.64925811305459	25.369868227011082
40	25.418677864861817	24.991772741283505	25.33096388282298	24.258585511031693
41	24.374842378756274	25.224938140789412	25.71981182548697	24.680407654967343
42	25.463186519937985	25.47895679011139	25.067821936706476	23.990034753244146
43	24.678582995608437	24.54473119835155	25.37240971683242	25.404276089207595
44	24.34480066574	25.118456233916113	25.23816692114148	25.298576179202403
45	24.46698767638102	25.292580869880283	25.365111079396797	24.8753203743419
46	24.494486581876263	24.821036291917586	25.313825936166733	25.370651190039418
47	24.623712882029906	24.659684737962625	25.766731637573127	24.949870742434342
48	25.27928692312254	25.436142461582772	24.787476059491713	24.49709455580297
49	24.22678430506219	25.223374147053207	25.47797929402626	25.07186225385834
50	24.03467374113167	25.61176592487747	25.17541167247625	25.17814866151461
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	7.5
2	10.0
3	43.5
4	77.0
5	86.0
6	95.0
7	90.5
8	86.0
9	133.5
10	181.0
11	279.5
12	378.0
13	630.0
14	882.0
15	1236.5
16	1591.0
17	2266.0
18	2941.0
19	3357.5
20	3774.0
21	5022.0
22	6270.0
23	7080.0
24	7890.0
25	10129.5
26	12369.0
27	16024.0
28	19679.0
29	22313.5
30	24948.0
31	26193.5
32	27439.0
33	31436.0
34	35433.0
35	41316.5
36	47200.0
37	55663.0
38	64126.0
39	67743.5
40	71361.0
41	77399.0
42	83437.0
43	83579.5
44	83722.0
45	90446.0
46	97170.0
47	102737.0
48	108304.0
49	110346.0
50	112388.0
51	107987.5
52	103587.0
53	101487.5
54	99388.0
55	100903.0
56	102418.0
57	101123.5
58	99829.0
59	91139.0
60	82449.0
61	72586.5
62	62724.0
63	55457.0
64	48190.0
65	41434.5
66	34679.0
67	30728.5
68	26778.0
69	24815.0
70	22852.0
71	18035.0
72	13218.0
73	11693.0
74	10168.0
75	7671.0
76	5174.0
77	4598.5
78	4023.0
79	3471.5
80	2920.0
81	2331.5
82	1743.0
83	1472.0
84	1201.0
85	997.0
86	793.0
87	612.0
88	431.0
89	279.0
90	127.0
91	87.5
92	48.0
93	34.5
94	21.0
95	15.5
96	10.0
97	7.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012968114729367175
2	0.0027369890383589016
3	7.168304624273314E-4
4	6.516640567521193E-5
5	0.0
6	5.213312454016955E-4
7	1.3033281135042387E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.6066562270084774E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1534533.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.146740225271785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1135067100007	45.2829222352252
2	11.069574739507736	12.209019251277274
3	2.830677611050627	4.683079286343054
4	1.1615370934310325	2.562199374138335
5	0.6592935432128104	1.8178944879877927
6	0.42140154411998393	1.3943352890467897
7	0.2718884952668205	1.0495634953103572
8	0.20218140503109513	0.8919716337304209
9	0.15906623057050082	0.7894785686296134
>10	0.8995674774530625	9.252319553215253
>50	0.10335566128188943	3.9944142765209834
>100	0.09676186515174537	11.153129359321408
>500	0.008926295682486906	3.3170185660720337
>1k	0.0022613282395633496	1.6026546231815053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1692	0.1102615584024586	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1671	0.10889306388327914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.516640567521193E-5
2	0.0	0.0	0.0	0.0	6.516640567521193E-5
3	0.0	0.0	0.0	0.0	6.516640567521193E-5
4	0.0	0.0	0.0	0.0	6.516640567521193E-5
5	0.0	0.0	0.0	0.0	6.516640567521193E-5
6	0.0	0.0	0.0	0.0	6.516640567521193E-5
7	0.0	0.0	0.0	0.0	6.516640567521193E-5
8	0.0	0.0	0.0	0.0	6.516640567521193E-5
9	0.0	0.0	0.0	0.0	6.516640567521193E-5
10	0.0	0.0	0.0	0.0	6.516640567521193E-5
11	0.0	0.0	0.0	0.0	6.516640567521193E-5
12	0.0	0.0	0.0	0.0	1.9549921702563582E-4
13	0.0	0.0	0.0	0.0	2.6066562270084774E-4
14	0.0	0.0	0.0	0.0	2.6066562270084774E-4
15	0.0	0.0	0.0	0.0	4.5616483972648356E-4
16	0.0	0.0	0.0	0.0	5.213312454016955E-4
17	0.0	0.0	0.0	0.0	5.213312454016955E-4
18	0.0	0.0	0.0	0.0	5.864976510769074E-4
19	0.0	0.0	0.0	6.516640567521193E-5	5.864976510769074E-4
20	0.0	0.0	0.0	1.9549921702563582E-4	5.864976510769074E-4
21	0.0	0.0	0.0	2.6066562270084774E-4	5.864976510769074E-4
22	0.0	0.0	0.0	4.5616483972648356E-4	5.864976510769074E-4
23	0.0	0.0	0.0	8.471632737777552E-4	6.516640567521193E-4
24	0.0	0.0	0.0	0.0013684945191794508	7.168304624273314E-4
25	0.0	0.0	0.0	0.0017594929532307223	7.168304624273314E-4
26	0.0	0.0	0.0	0.0022156577929572058	8.471632737777552E-4
27	0.0	0.0	0.0	0.0028021554440341135	8.471632737777552E-4
28	0.0	0.0	0.0	0.006255974944820346	8.471632737777552E-4
29	0.0	0.0	0.0	0.012511949889640692	8.471632737777552E-4
30	0.0	0.0	0.0	0.025349731807657445	8.471632737777552E-4
31	0.0	0.0	0.0	0.057607102616887354	8.471632737777552E-4
32	0.0	0.0	0.0	0.09814060694686917	8.471632737777552E-4
33	0.0	0.0	0.0	0.14049877063575694	8.471632737777552E-4
34	0.0	0.0	0.0	0.1878095811559608	8.471632737777552E-4
35	0.0	0.0	0.0	0.24600318142392508	8.471632737777552E-4
36	0.0	0.0	0.0	0.3336519970570851	8.471632737777552E-4
37	0.0	0.0	0.0	0.45655583816053485	8.471632737777552E-4
38	0.0	0.0	0.0	0.626965989001214	8.471632737777552E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	60	2.8734966E-4	21.99974	5
GTTATAT	265	0.0	20.76056	1
ATCGTTT	415	0.0	19.08411	29
GTATCAA	2450	0.0	18.68281	1
GTACTAA	125	4.840276E-7	17.604954	1
CACGACT	75	0.0012911883	17.59979	44
CCGTTAT	75	0.0012911883	17.59979	9
GTCCTAA	290	0.0	17.453188	1
ATTTCGT	115	4.2748816E-6	17.217188	42
ACCGTCG	385	0.0	17.142654	8
TATATCA	310	0.0	17.032057	4
CGTTTAT	480	0.0	16.958132	31
TGGTCGG	530	0.0	16.603577	37
CATCGTT	465	0.0	16.558943	28
CGCAATA	455	0.0	16.439365	36
TAGGACG	295	0.0	16.406586	4
ATACCGT	370	0.0	16.053865	6
GCTATAT	110	5.6093064E-5	16.004505	1
CCCTATA	165	3.101377E-8	16.004503	1
ATAACGC	550	0.0	16.000332	3
>>END_MODULE