FastQCFastQC Report
Thu 2 Feb 2017
SRR4062383_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062383_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699764
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG50020.7148124224738626No Hit
TCCATGTACTCTGCGTTGATACCAC39930.570620952206744No Hit
GAGTACATGGAAGCAGTGGTATCAA23440.3349700756254966No Hit
CATGTACTCTGCGTTGATACCACTG19650.28080895844884846No Hit
GTATCAACGCAGAGTACTTTTTTTT18950.2708055858832406No Hit
GCTTCCATGTACTCTGCGTTGATAC13110.18734887762159813No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12880.18406205520718413No Hit
TATCAACGCAGAGTACTTTTTTTTT12830.18334752859535502No Hit
GCGTTGATACCACTGCTTCCATGTA11670.16677051120091915No Hit
CATGGAAGCAGTGGTATCAACGCAG11610.16591307926672422No Hit
CCCATGTACTCTGCGTTGATACCAC11470.16391240475360264No Hit
GTACATGGGAAGCAGTGGTATCAAC10860.15519518008928723No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10200.14576342881314272No Hit
GTATCAACGCAGAGTACATGGAAGC8860.126614115616122No Hit
ACTCTGCGTTGATACCACTGCTTCC7770.11103743547824696No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7690.10989419289932036No Hit
GCGCAAGACGGACCAGAGCGAAAGC7670.1096083822545887No Hit
GGGTAGGCACACGCTGAGCCAGTCA7670.1096083822545887No Hit
GTCTTGCGCCGGTCCAAGAATTTCA7580.10832223435329624No Hit
GAATAGGACCGCGGTTCTATTTTGT7520.10746480241910131No Hit
CAGTGGTATCAACGCAGAGTACATG7400.10574993855071138No Hit
ACGCAGAGTACATGGAAGCAGTGGT7020.10031953630080999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACT250.00603460418.9968514
TGCGGAT402.7558545E-416.62700515
CGGATAT350.002166847216.28767817
ATTTGCG704.4197986E-714.93037116
AGTGCAC456.749572E-414.77955910
TATCGAA456.764236E-414.775334
ATGCGGA400.0052710414.25171814
CGTTATT602.5769612E-514.2425452
GTACTAG400.00529611714.2415271
GTATAAT400.00529611714.2415271
AACCGCG759.645464E-713.9340157
GTCCTAG759.715768E-713.9250491
CGGATTA551.954536E-413.82083618
ATCGGAT551.955723E-413.81984616
ATCGAAC551.9569107E-413.8188588
AATCGAA551.9569107E-413.8188587
GCGTTAT551.9676282E-413.8099651
CAACGGA500.001496748113.30160214
CCGTCAC500.001496748113.30160211
CCCGTCA500.001496748113.30160210
Produced by FastQC (version 0.11.5)