##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062383_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	699764
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133936584334148	32.0	32.0	32.0	32.0	32.0
2	30.801948942786424	32.0	32.0	32.0	32.0	32.0
3	30.80425543468941	32.0	32.0	32.0	32.0	32.0
4	30.85274892678103	32.0	32.0	32.0	32.0	32.0
5	30.665834481339424	32.0	32.0	32.0	32.0	32.0
6	34.30814388851098	36.0	36.0	36.0	32.0	36.0
7	34.188947988178874	36.0	36.0	36.0	32.0	36.0
8	34.1555824535129	36.0	36.0	36.0	32.0	36.0
9	34.32427218319319	36.0	36.0	36.0	32.0	36.0
10	33.98924923259842	36.0	36.0	36.0	32.0	36.0
11	34.329648281420596	36.0	36.0	36.0	32.0	36.0
12	34.10703322834556	36.0	36.0	36.0	32.0	36.0
13	34.222700796268455	36.0	36.0	36.0	32.0	36.0
14	34.137760730760654	36.0	36.0	36.0	32.0	36.0
15	34.06876032490954	36.0	36.0	36.0	32.0	36.0
16	34.05193751036064	36.0	36.0	36.0	32.0	36.0
17	33.99454101668562	36.0	36.0	36.0	32.0	36.0
18	34.00433574748058	36.0	36.0	36.0	32.0	36.0
19	33.98250553043597	36.0	36.0	36.0	32.0	36.0
20	33.96891523427899	36.0	36.0	36.0	32.0	36.0
21	33.93319604895365	36.0	36.0	36.0	32.0	36.0
22	33.86397271079964	36.0	36.0	36.0	32.0	36.0
23	33.81933051714578	36.0	36.0	36.0	32.0	36.0
24	33.790995249827084	36.0	36.0	36.0	32.0	36.0
25	33.30221188857958	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	7.0
4	25.0
5	80.0
6	121.0
7	31.0
8	115.0
9	125.0
10	90.0
11	25.0
12	60.0
13	41.0
14	132.0
15	191.0
16	271.0
17	317.0
18	509.0
19	733.0
20	1058.0
21	1440.0
22	2244.0
23	3220.0
24	4500.0
25	6156.0
26	8517.0
27	10852.0
28	14684.0
29	19897.0
30	25702.0
31	35169.0
32	49845.0
33	71510.0
34	155913.0
35	286182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.8896355484923	17.137267063879413	10.78276826509306	25.190329122535225
2	16.343881030436933	19.98379402694136	37.23286582769077	26.43945911493093
3	19.803553641174563	24.165217304314645	27.853624168075537	28.177604886435255
4	12.55572500753958	14.843612652774778	35.55337666031579	37.04728567936984
5	13.918795470433585	37.19851378069545	33.85795140194398	15.024739346926989
6	34.24418978717929	34.95278196778737	16.97761700849996	13.82541123653339
7	29.895991682766766	30.343314680574483	21.09883006731296	18.66186356934579
8	28.734471438890598	32.65943371262239	19.70977593099848	18.89631891748854
9	27.20299717191253	14.215395267066155	18.63376338763967	39.94784417338165
10	15.741737015309774	27.09829732436686	31.728144226641863	25.4318214336815
11	37.618845264002815	21.339565868713272	21.30765917932561	19.7339296879583
12	23.980469431970715	23.89933056860169	29.41720258385065	22.702997415576945
13	30.057719933838133	19.85399747028153	25.244386829438596	24.84389576644174
14	23.540076008196063	19.787600590666102	24.885529825204042	31.786793575933793
15	25.43023768235382	27.152905356294603	23.19076970623079	24.226087255120788
16	26.10245830102241	25.698416740826797	24.637092274278977	23.562032683871816
17	23.662792860597353	25.75856868541471	26.27138773068987	24.30725072329807
18	25.051623252572575	24.46920706970248	27.386725776423738	23.09244390130121
19	25.037742788086586	26.222902109160014	25.10299667004861	23.63635843270479
20	24.7649109151161	24.34595726901746	26.787993429524786	24.101138386341653
21	25.746000082996222	24.96254437793617	24.105966140403847	25.185489398663762
22	24.734179208083756	25.298525086412333	25.235120261634357	24.732175443869554
23	23.547115267650785	24.93656665688751	25.448183581032254	26.068134494429458
24	23.840563947864016	26.400138525561403	25.2206677847931	24.538629741781484
25	24.944512894044312	24.901582831409353	25.084751098651854	25.06915317589448
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	87.0
2	259.0
3	431.0
4	431.0
5	431.0
6	1193.0
7	1955.0
8	1955.0
9	1955.0
10	1992.5
11	2030.0
12	2030.0
13	2030.0
14	1926.0
15	1822.0
16	1822.0
17	1822.0
18	2932.0
19	4042.0
20	4042.0
21	4042.0
22	6634.5
23	9227.0
24	9227.0
25	9227.0
26	14567.0
27	19907.0
28	19907.0
29	19907.0
30	25456.0
31	31005.0
32	31005.0
33	31005.0
34	37130.5
35	43256.0
36	43256.0
37	43256.0
38	49671.5
39	56087.0
40	56087.0
41	56087.0
42	65460.5
43	74834.0
44	74834.0
45	74834.0
46	92312.0
47	109790.0
48	109790.0
49	109790.0
50	102205.5
51	94621.0
52	94621.0
53	94621.0
54	85506.5
55	76392.0
56	76392.0
57	76392.0
58	68843.5
59	61295.0
60	61295.0
61	61295.0
62	53986.5
63	46678.0
64	46678.0
65	46678.0
66	38840.5
67	31003.0
68	31003.0
69	31003.0
70	23760.5
71	16518.0
72	16518.0
73	16518.0
74	12884.0
75	9250.0
76	9250.0
77	9250.0
78	7370.0
79	5490.0
80	5490.0
81	5490.0
82	3823.5
83	2157.0
84	2157.0
85	2157.0
86	1611.5
87	1066.0
88	1066.0
89	1066.0
90	732.0
91	398.0
92	398.0
93	398.0
94	238.5
95	79.0
96	79.0
97	79.0
98	211.5
99	344.0
100	344.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00785979273012044
2	0.0031439170920481764
3	0.004430064993340612
4	0.01757735465099662
5	0.038727342361138894
6	0.04987395750567335
7	0.07045232392635231
8	0.0927455542154212
9	0.10074825226790746
10	0.12347019852407383
11	0.12161242933331809
12	0.1374749201159248
13	0.12461344110300043
14	0.12747154755031698
15	0.12118371336622062
16	0.11803979627417244
17	0.1260424943266587
18	0.1359029615699007
19	0.136474582859364
20	0.12575668368192705
21	0.13390228705677915
22	0.15419484283272644
23	0.14276241704346038
24	0.13947559462904635
25	0.13618877221463235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	699764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.20226244660907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.13647713550512	41.83305410974325
2	12.24129463314044	12.780465502509289
3	3.411824901252527	5.343149367511814
4	1.3349689971039258	2.787536077796226
5	0.6958774862179905	1.8163189583119064
6	0.41088785696701596	1.2869565447310165
7	0.27074816239033217	0.9893566641026076
8	0.19329735226060943	0.8072447290354381
9	0.14582742394285922	0.6851269310920256
>10	0.9051202478652358	9.243901397675772
>50	0.13150512439759418	4.883369245267559
>100	0.11173812659602178	11.688323399308523
>500	0.006863521289678329	2.3207246653213858
>1k	0.003294490219045598	2.8176030951780673
>5k	2.745408515871332E-4	0.7168693124150912
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5002	0.7148124224738626	No Hit
TCCATGTACTCTGCGTTGATACCAC	3993	0.570620952206744	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2344	0.3349700756254966	No Hit
CATGTACTCTGCGTTGATACCACTG	1965	0.28080895844884846	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1895	0.2708055858832406	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1311	0.18734887762159813	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1288	0.18406205520718413	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1283	0.18334752859535502	No Hit
GCGTTGATACCACTGCTTCCATGTA	1167	0.16677051120091915	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1161	0.16591307926672422	No Hit
CCCATGTACTCTGCGTTGATACCAC	1147	0.16391240475360264	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1086	0.15519518008928723	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1020	0.14576342881314272	No Hit
GTATCAACGCAGAGTACATGGAAGC	886	0.126614115616122	No Hit
ACTCTGCGTTGATACCACTGCTTCC	777	0.11103743547824696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	769	0.10989419289932036	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	767	0.1096083822545887	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	767	0.1096083822545887	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	758	0.10832223435329624	No Hit
GAATAGGACCGCGGTTCTATTTTGT	752	0.10746480241910131	No Hit
CAGTGGTATCAACGCAGAGTACATG	740	0.10574993855071138	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	702	0.10031953630080999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.429053223658262E-4
2	0.0	0.0	0.0	0.0	1.429053223658262E-4
3	0.0	0.0	0.0	0.0	1.429053223658262E-4
4	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
5	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
6	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
7	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
8	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
9	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
10	0.0	0.0	0.0	1.429053223658262E-4	1.429053223658262E-4
11	0.0	0.0	0.0	2.858106447316524E-4	1.429053223658262E-4
12	0.0	0.0	0.0	4.287159670974786E-4	1.429053223658262E-4
13	0.0	0.0	0.0	4.287159670974786E-4	1.429053223658262E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCACT	25	0.006034604	18.996851	4
TGCGGAT	40	2.7558545E-4	16.627005	15
CGGATAT	35	0.0021668472	16.287678	17
ATTTGCG	70	4.4197986E-7	14.930371	16
AGTGCAC	45	6.749572E-4	14.779559	10
TATCGAA	45	6.764236E-4	14.77533	4
ATGCGGA	40	0.00527104	14.251718	14
CGTTATT	60	2.5769612E-5	14.242545	2
GTACTAG	40	0.005296117	14.241527	1
GTATAAT	40	0.005296117	14.241527	1
AACCGCG	75	9.645464E-7	13.934015	7
GTCCTAG	75	9.715768E-7	13.925049	1
CGGATTA	55	1.954536E-4	13.820836	18
ATCGGAT	55	1.955723E-4	13.819846	16
ATCGAAC	55	1.9569107E-4	13.818858	8
AATCGAA	55	1.9569107E-4	13.818858	7
GCGTTAT	55	1.9676282E-4	13.809965	1
CAACGGA	50	0.0014967481	13.301602	14
CCGTCAC	50	0.0014967481	13.301602	11
CCCGTCA	50	0.0014967481	13.301602	10
>>END_MODULE